ABSTRACT
A technology for obtaining a biomatrix on the basis of polypropylene mesh, used in clinical surgery, and cultured fetal fibroblasts is proposed. The method was developed on experimental animal cells. It includes culturing of rat fetal fibroblasts on fibers of a polypropylene mesh, treated with wheat germ lectin. This technology can be used for obtaining a biomatrix with fibroblasts of different origin. This biomatrix can be used in experimental and clinical surgery for dressing and stimulation of effective healing of mechanical injuries to the connective tissue.
Subject(s)
Biocompatible Materials/metabolism , Fibroblasts/physiology , Polypropylenes/metabolism , Surgical Mesh , Animals , Biocompatible Materials/chemistry , Cell Culture Techniques , Cells, Cultured , Fibroblasts/cytology , Materials Testing , Mice , Polypropylenes/chemistry , Rats , Wheat Germ Agglutinins/metabolismABSTRACT
A possibility to evaluate the quantity of cultured cells was examined using the optical density of the stain eluate after absorbtion by cells in the course of staining of the cellular monolayer. The investigation of the growth dynamics of primary trypsinized cultures of embryonic fibroblasts using the proposed criteria has shown its relationship with the quantity of cells in culture. It is proposed that this method may be used for quantitative characteristics of cell cultures.
