[Urethritis of chlamydial origin]. / Uretritídy chlamýdiového pôvodu.

Using the direct immunofluorescence method with a Chlamyset of Orion Co. Finland for detection of Chlamydia trachomatis, the authors examined smears from male and female patients with manifestations of non-specific urethritis and cervicitis. In women also specimens from the cervix were examined. The IF examinations of smears from the urethra of 72 men were positive in 25, i.e. 34.7%. IF examinations of 70 women with manifestations of cervicitis and urethritis were positive in 17, i.e. 24.3% from the urethra and in 20 cases, i.e. 28.6% from the cervix. The positive findings of Chlamydia trachomatis indicate the importance of diagnosing urethritis of chlamydial origin and aimed treatment.

[Encephalitozoon cuniculi--clinical and epidemiologic significance. Results of a preliminary serologic study in humans]. / Encephalitozoon cuniculi--klinický a epidemiologický význam. Výsledky orientacného sérologického vysetrenia u l'udí.

Recently attention in human medicine has been drawn to encephalitozoonoses. Increased liability to develop the infection is found in particular in people with an impaired immunobiological condition. Evidence of this are more numerous infections in AIDS patients. However, the impact of encephalitozoonoses cannot be ruled out in other groups of patients. This calls however for specific aimed investigations. The authors examined 98 sera of employees of a slaughterhouse for encephalitozoonosis. Five sera (5.1%) were positive. Sera of 92 forestry workers, 22 dog breeders and 150 blood donors were negative. In future it will be necessary to focus attention on seropositive subjects in conjunction with the possible transmission of the disease from animals to man.

[Relation between characteristics of the small mammal population and occurrence of Leptospira antibodies]. / Vztah struktúry populácií drobných cicavcov a výskyt protilátok proti leptospíram.

Five rodent species (Apodemus flavicollis, A. agrarius, A. microps, Clethrionomys glareolus and Microtus arvalis) were examined serologically for presence of antibodies to leptospira. Small mammals were caught in several areas of eastern Slovakia, an important part of host material coming from East Slovakian Lowland (45.7%), south part of Kosice basin (26.5%) and Slovak carst (10.1%). Serological examinations were used by Kmety and Bakoss (1978), the sexual condition of rodents were ascertained after Pelikán (1965). Altogether, 2,113 individuals of rodent species were examined during five years (1991-1995), antibodies to leptospires were demonstrated in 5.02% of examined hosts. In examined hosts antibodies to six serovars of leptospira (Leptospira grippothyphosa, L. sejroe, L. bataviae, L. bratislava, L. pomona and L. sorex-jalna) were found. The most frequently observed antibodies were those of the serovar L. grippothyphosa (65.1%) and L. sejroe (25.5%). Slightly higher percentage of positive hosts in mature group rodents (5.4%) comparing with immature rodents (4.6%) were statistically insignificant. Similarly, no differences were found between percentage of positive rodent females (5.3%) and group of males (4.7%). No statistical differences were found in positivities to leptospira antibodies among sexual and age groups of individual rodent species, too. Proportion of serological positivities to L. grippothyphosa and L. sejroe in compared sex and age rodent groups were very similar. In evaluated rodent species trend of increases of serological positive values from spring to autumn was observed, but it was statistically confirmed only in A. agrarius.

[Leptospira antibodies in small mammals in Eastern Slovakia]. / Výskyt protilátok proti leptospíram u drobných cicavcov na východnom slovensku.

During the five years (1991-1995) mostly free living small mammals were examined serologically for the presence of antibodies to leptospira. Serological examinations were used by Kmety and Bakoss (1978). Altogether, 2493 individuals of 22 species were examined, an important part of host material (69.6%) was caught in two lowland areas which are intensively cultivated landscapes (East Slovakian Lowland and Kosická kotlina basin). The remaining material comes from submontane areas with less agricultural activities. Apodemus flavicollis, A. agrarius, Clethrionomys glareolus were the most frequently examined species. Antibodies to leptospira were demonstrated in 123 mammals (i. e. in 5.0% of hosts) of eleven species. The highest percentage of positive hosts were recorded in 1993 (8.8%) and the lowest (2.6%) in 1995, but the examined samples were different in size and structure of host species. In six most dominant species higher values of antibodies to leptospira were detected in Microtus arvalis (9.2%), followed by Apodemus flavicollis (5.6%), A. agrarius (5.4%) and A. microps (4.9%). The lowest values were found in Clethrionomys glareolus (2.5%) and Sorex araneus (2.3%). In examined hosts we found antibodies to six serovars of leptospira. The most frequently observed antibodies were to leptospira of the serovar L. grippotyphosa (63.2%) and leptospira of the serovar L. sejroe (26.4%). We recorded nonsignificant differences between the values of prevalence to antibodies in small mammal communities from lowland and submontane areas, resp. Our results confirmed differences in the leptospira serovar structure in small mammals of Bohemia and Slovakia, they were connected with different historical development of both areas. Comparing our results with previous examinations (about 20 years ago) in small mammals from eastern Slovakia, a decline of positivity and lower number of leptospira serovars were recorded.

[Extra-urogenital detection of Chlamydia trachomatis--perinatal transmission]. / Extraurogenitálne dôkazy Chlamydia trachomatis--perinatálny prenos.

Infections produced by Chlamydia trachomatis reach an epidemic scale worldwide. Examination of 1,333 women with suspected cervicitis and other clinical symptoms revealed positive findings in 355 (27.5%). Of 72 examined men 25 (34.7%) were positive. Perinatal infection is confirmed also by positive evidence of Ch. t. in 28.9% neonates with conjunctivitis and 19% positive smears from the nasopharynx. The authors used the direct immunofluorescent method, Chlamyset of Orion Co., Finland.

[Occurrence of Leptospira serovars in old foci of leptospirosis]. / Súcasný stav výskytu leptospírových sérovarov v starých ohniskách leptospiróz.

In two old natural foci of leptospiroses in an Eastern Slovakian area an epidemiological investigation was made to detect persisting carriership in basis reservoirs-small mammals and the contact with leptospirae in population groups with an occupational risk. Using the microagglutination reaction (MAR), the authors examined 1,106 small mammals and detected in 50 cases, i.e. 4.5%, the presence of specific antibodies against leptospirae, most frequently in species Apodemus agrarius and Ap. flavicollis. Only in one instance a positive reaction was found in the main reservoir--Microtus arvalis. As to serovars, Leptospira grippotyphosa and the group L. sejroe dominated. Of 1,740 examined human sera (832 men, 908 women) 56 samples (3.2%) reacted in MAR (4.1% men, 2.4% women). The spectrum of serovars in positive subjects was the same as in the above mentioned reservoirs with a predominance of L. grippotyphosa and group L. sejroe. As compared with previous examinations made 18 years ago in small mammals, a decline of positivity to almost half was recorded (from 7.8% to 4.5%), a reduction of the number of serovars from 7 to 4 was observed, however, the positivity of dominating serovars did not decline. In humans a higher percentage of positivity to specific antibodies was found, as compared with the past, i.e. 3.0% as compared with 2.0%. The authors found differences in the frequency of different serovars. In the past serovar L. bratislava dominated in the above area, followed by L. grippotyphosa. At present the first place was held by L. grippotyphosa and the group of L. sejroe.(ABSTRACT TRUNCATED AT 250 WORDS)

[Examination of ticks for the presence of Borrelia sp. in Kosice and the surrounding region. Preliminary results]. / Vysetrenie kliestov na prítomnost Borrelia sp. v Kosiciach a okolí. Predbezné výsledky.

The authors present preliminary data on the infestation of ticks Ixodes ricinus with Borrelia in the town of Kosice and surroundings. The authors found a mean 4.7% positivity which means that more systematic attention should be paid to the problem.

[Detection of Chlamydia trachomatis in clinical material 1985-1988]. / Dôkaz Chlamydia trachomatis z klinického materiálu za roky 1985-1988.

Investigation of 529 smears from the uterine cervix of women before delivery, during the puerperium and before induced abortion revealed positive findings in 25.2% of women before delivery and in 19.3% of women before induced abortion. Perinatal infection was confirmed by a positive finding of Ch. t. in 12.8% neonates with conjunctivitis and 15.4% from the nasopharynx. The direct immunofluorescent method was used. In another group of women examined by the serological method ELISA, from a total of 182 sera in 41.7% the presence of IgG antibodies was detected. In cervical smears from 10 seropositive women in seven Ch. t. was positive.