ABSTRACT
Contribution of various hemoproteins to peroxidase oxidation of benzidine and its derivatives as well as effects of these substances on functional state of hepatocytes are discussed. Benzidine and its derivatives were shown to induce those forms of cytochrome P-450 which were involved in accelerated oxidation of the carcinogenic drugs studied as well as affected the glutathione transferase, NADPH-dependent glutathione reductase activities and the activity of antioxidant system enzymes. Increase in content of cytochrome P-450, glutathione-dependent enzymes and other effects specific for adult hepatocytes, which occurred in presence of aminobiphenyls, were accompanied by decrease in content of receptors to epidermal factor of growth regulating the hepatocytes proliferation.
Subject(s)
Aminobiphenyl Compounds/toxicity , Cell Differentiation , Liver/cytology , Animals , Antioxidants , Benzidines , Carcinogens/toxicity , Dianisidine/toxicity , Glutathione/metabolism , Glutathione Reductase/metabolism , Hemeproteins/metabolism , Kinetics , Liver/drug effects , Male , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , Mixed Function Oxygenases/metabolism , NADP/metabolism , Peroxidases/metabolism , RatsABSTRACT
Photodestruction of erythrocyte membranes sensitized by water soluble chlorophyll derivative chlorine e6 (Chl e6) was studied. It has been determined that light irradiation of erythrocyte ghosts with wave length lambda-660 nm in the presence of Chl e6 caused in protein and lipid components of the membrane deep destructive changes which were expressed in cross-linking of the membrane polypeptides and accumulating in the membrane products of peroxidation of unsaturated fatty acids residues, in phospholipids mainly. It has been shown that these processes were realized only in the presence of molecular oxygen and consequently possess pure photodynamic character.
