ABSTRACT
It is shown that Wistar male rats keeping without medication and stressful effects for a month after administration of big doses of dopamine for two weeks does not lead to complete inverse development of heart remodeling. A decrease of the heart's mass occurs mainly because of a decrease of the right ventricle mass. The greatest changes of the extracellular matrix take place in the right ventricle: collagen level grows more noticeably, edema of the extracellular space relieves, correlations between collagen and blood vessels, cardiac hystiocytes and blood vessels increase aggravating right ventricular remodeling.
Subject(s)
Dopamine/pharmacology , Heart Ventricles/drug effects , Sympathomimetics/pharmacology , Ventricular Remodeling/drug effects , Animals , Dopamine/administration & dosage , Dose-Response Relationship, Drug , Fibrosis , Heart Ventricles/pathology , Injections, Intraperitoneal , Male , Rats , Rats, Wistar , Sympathomimetics/administration & dosageSubject(s)
Antioxidants/pharmacology , Connective Tissue Cells/drug effects , Dehydration/metabolism , Macrophages/drug effects , Vitamin E/pharmacology , Animals , Connective Tissue Cells/enzymology , Connective Tissue Cells/metabolism , Connective Tissue Cells/ultrastructure , Dehydration/enzymology , Dehydration/pathology , Lipid Peroxidation , Macrophages/enzymology , Macrophages/metabolism , Male , Oxidation-Reduction , Rats , Water DeprivationABSTRACT
The morphological aspects of rearrangement of macrophages in white rats' subcutaneous areolar tissue during dehydration and their impact on the recovery of osmotic homeostasis were studied. A response of macrophages to dehydration with preliminary injection of antioxidant dibunol was revealed. Macrophages respond to dehydration by a rise in number, elevated cytoplasmic activity of lipolytic enzymes (beta-oxibutyrate dehydrogenase), and a peculiar ultrastructural rearrangement, i.e. a decrease of vacuoles, their sizes, an increase of the lipid volumetric fraction. However, the higher activity of macrophages in terms of production of metabolic water is concurrent to increased malonic dialdehyde production and inhibited activity of the cell antioxidant defense (superoxide dismutase). To correct the observed changes, dehydration was performed on the background of antioxidant dibunol injection. The antioxidant and subsequent dehydration did not annul the response of macrophages to increased intrabody osmolality although slightly masked the effect of dehydration. Survivability in this group of animals was by 22% higher than in the water-deprived group. Hence, the areolar macrophages can be considered the controllers of adaptive reactions of the body during dehydration, whereas antioxidants are the correction factor for these reactions.
