Α Quantum Pattern Recognition Method for Improving Pairwise Sequence Alignment.

Quantum pattern recognition techniques have recently raised attention as potential candidates in analyzing vast amount of data. The necessity to obtain faster ways to process data is imperative where data generation is rapid. The ever-growing size of sequence databases caused by the development of high throughput sequencing is unprecedented. Current alignment methods have blossomed overnight but there is still the need for more efficient methods that preserve accuracy in high levels. In this work, a complex method is proposed to treat the alignment problem better than its classical counterparts by means of quantum computation. The basic principal of the standard dot-plot method is combined with a quantum algorithm, giving insight into the effect of quantum pattern recognition on pairwise alignment. The central feature of quantum algorithmic -quantum parallelism- and the diffraction patterns of x-rays are synthesized to provide a clever array indexing structure on the growing sequence databases. A completely different approach is considered in contrast to contemporary conventional aligners and a variety of competitive classical counterparts are classified and organized in order to compare with the quantum setting. The proposed method seems to exhibit high alignment quality and prevail among the others in terms of time and space complexity.

Solid phase synthesis of a glycopeptide analogue using the acid sensitive 4-methoxybenzhydryl bromide resin.

A convenient solid phase synthesis of a Thrombin Receptor Glycopeptide Mimetic analogue namely, 1-O-Methyl-2-N-{1'-(argininocarbonyl)-4'-[(4''-fluoro)-benzylamido]-cyclohexane}-glucosamine using Fmoc/tBu methodology and the 4-Methoxybenzhydryl bromide resin is described. The synthesized analogue was purified by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) and was identified by Electron Spray Ionization-Mass Spectrometry (ESI-MS) and Nuclear Magnetic Resonance (NMR). The synthetic protocol introduced for the first time successfully the acid sensitive 4-Methoxybenzhydryl bromide resin as a scaffold for the synthesis of glycopeptides resulting in high yield reactions. This synthetic procedure could be a general one for the convenient synthesis of such glyco compounds as the method was used for the first time to glycosylate a non peptide mimetic of an important protein sequence, in particular of the thrombin receptor active site S42FLLR46.

Determination of carbendazim, thiabendazole, and o-phenylphenol residues in lemons by HPLC following sample clean-up by ion-pairing.

An efficient analytical method is presented involving effective sample clean-up with solid-phase extraction and HPLC-UV analysis for the simultaneous determination of carbendazim, thiabendazole, and o-phenylphenol residues in lemons. Sample preparation involves extraction with acetonitrile acidified with trifluoroacetic acid and an ethyl acetate/petroleum ether mixture. Purification of the crude extract was carried out with liquid-liquid partitioning after addition of an aqueous ammonia solution. Final clean-up was performed on polymeric reversed-phase cartridges pretreated with sodium dodecyl sulfate. Chromatographic analysis was performed on a reversed-phase HPLC column isocratically eluted with an acetonitrile/water/ammonia mixture and UV detection at 254 nm. The chromatographic method is repeatable, reproducible, and sensitive. Fungicide recoveries from lemon samples fortified at levels of 5 and 1 mg kg(-1) were 81-85% for carbendazim, 96-98% for thiabendazole, and 81-106% for o-phenylphenol with coefficients of variation of 2.5-7.4%. Detection limits for carbendazim, thiabendazole, and o-phenylphenol in lemons were 0.21, 0.27, and 0.51 mg kg(-1), respectively.