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1.
Lab Delo ; (12): 74-5, 1990.
Article in Russian | MEDLINE | ID: mdl-1710703

ABSTRACT

A method for detection of lipase in intestinal bacteria is suggested with fatty acid esters used as enzyme substrates. This modification not only allows the enzyme detection but is more rapid that the known procedures. Preliminary cultivation of bacteria in the accumulation media rich in organic substances helps shorten the time of investigation and simplifies reading the reaction.


Subject(s)
Lipase/metabolism , Salmonella/enzymology , Shigella/enzymology
2.
Zh Mikrobiol Epidemiol Immunobiol ; (7): 97-101, 1983 Jul.
Article in Russian | MEDLINE | ID: mdl-6353820

ABSTRACT

The methodological principles of using Soviet bentonite clays as immunosorbents necessary for serological studies in bacterial infections were worked out. The method for the preparation of antigenic bentonite diagnostic reagents was experimentally substantiated. Bentonite diagnostic reagents were first used in a new method for the rapid indication of bacterial antigens in environmental objects with a view of solving epidemiological problems.


Subject(s)
Antigen-Antibody Complex/immunology , Bentonite/immunology , Immunosorbent Techniques , Immunosorbents/immunology , Adsorption , Agglutination Tests/methods , Antigens, Bacterial/analysis , Erythrocytes/immunology , Evaluation Studies as Topic , Humans , Particle Size , Serologic Tests/methods , Suspensions , USSR
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