ABSTRACT
The applicability of glass chips with powder-blasted microchannels for electrophoretic separations was examined, and the performance was compared to microchannels etched with hydrogen fluoride (HF), using bicarbonate buffer and rhodamine B and fluorescein as model compounds. The measured electroosmotic mobilities in all chips were comparable, with values of ca. 7 x 10(-4) cm(2) V(-1)s(-1). The effect of electrical field strength and detection length on the separation efficiency was monitored. It was found that the main source of dispersion is of the Taylor-Aris type, which was discussed in relation to channel roughness differences. Although in powder-blasted channels with a separation length of 8.20 cm, 7-9 times lower plate numbers were obtained than in a HF-etched channel with similar dimensions, successful separation of five fluorescein isothiocyanate (FITC)-labeled amino acids was obtained on a powder-blasted chip within 80 s. Efficiencies of up to 360 000 plates/m were demonstrated on this chip, when a higher buffer concentration was used at a field strength of 664 V/cm. It can be concluded that powder-blasted microchannel chips, although they have a lower separation efficiency compared to HF-etched chips, perform well enough for many applications. Powder blasting can therefore be considered a low-cost and efficient alternative to HF etching, in particular because of the possibility to fabricate access holes through the glass with the same process.
Subject(s)
Electrophoresis, Capillary/instrumentation , Glass , Amino Acids/isolation & purification , Fluorescein-5-isothiocyanate , Fluorescent Dyes , Indicators and Reagents , Microscopy, Electron, Scanning , Miniaturization , Osmosis , Surface PropertiesABSTRACT
A novel, rapid, and simple capillary electrophoresis (CE) method has been developed for the determination of antimalarial artemisinin by on-line treatment with alkaline. By on-line reaction, artemisinin was automatically and reproducibly converted to the strongly UV-absorbing compound, Q292, by treating it with 0.20 mol/L NaOH solution for 3 min at 40 degrees C. Analysis was carried out in less than 12 min after conversion of artemisinin in a flow injection (FI) system that was coupled to CE equipment via a split-flow interface cell, and a sampling frequency of 8 h(-1) is achievable. The on-line conversion method has been applied to the determination of artemisinin in the traditional Chinese herbal drug Artemisia annua L., and the results are satisfactory.