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Appl Biochem Biotechnol ; 186(3): 597-612, 2018 Nov.
Article in English | MEDLINE | ID: mdl-29691792

ABSTRACT

Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as Mucuna pruriens L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from M. pruriens L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5-10, 3-5, 1-3, and < 1 kDa, respectively). At 300 µg/mL, F5-10 kDa inhibited 50.26 and 61.00% NO and H2O2 production, respectively. Moreover, F5-10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5-10 kDa decreased the inflammatory mediators.


Subject(s)
Enzymes/metabolism , Inflammation Mediators/metabolism , Macrophages/metabolism , Mucuna/chemistry , Plant Proteins/metabolism , Animals , Anti-Inflammatory Agents/pharmacology , Chlorocebus aethiops , Hydrolysis , Interleukin-6/biosynthesis , Male , Mice, Inbred BALB C , Molecular Weight , Peptides/chemistry , Peptides/metabolism , Plant Proteins/chemistry , Proteolysis , Transforming Growth Factor alpha/biosynthesis , Vero Cells
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