Correlates of perceived effectiveness of the Safe Schools/Healthy Students Initiative.

A three-level growth-curve model was applied to estimate perceived impact growth trajectories, using multi-year data from project and school surveys on outcome and program implementation collected from 59 sites and approximately 1165 participating schools in the Safe Schools and Healthy Students Initiative. Primary interest is to determine whether and how project-level and school-level correlates affect schools' perceptions of the Initiative's effectiveness over time when the effects of the pre-grant environmental conditions, grant operations, and near-term outcomes are considered. Coordination and service integration, comprehensive programs and activities for early childhood development, and change in school involvement were found to be significant predictors of school-perceived overall impact when the effect of poverty was considered. Partnership functioning, perceived importance of school resources, and school involvement were found to be significant predictors of school-perceived impact on substance use prevention when the effect of poverty was considered.

Moderators of the association between relational aggression and perceived popularity.

This study, which involved two waves of measurement over a period of 12 months, examined first whether the positive association between relational aggression and two types of high status was moderated by social self-efficacy, leadership, cooperation, and peer sociability. It was expected that relational aggressors are most likely to gain high peer group status when they are also self-efficacious or prosocial. Second, this study examined the reciprocal association between relational aggression and high peer status. It was anticipated that relational aggression would contribute to higher perceived popularity and vice versa. Third, we also examined and hypothesized reciprocal effects between social self-efficacy, leadership, cooperation, and peer sociability and perceived popularity. Youth were 540 13-15 year olds participating in Grade 7 (Time 1) and again 1 year later in Grade 8 (Time 2). Data were collected from peers, teachers, and the adolescents themselves. Consistent with expectations, relationally aggressive adolescents high in social self-efficacy, leadership, cooperation, and peer sociability were higher in status than relationally aggressive individuals with low levels of these characteristics. In addition, relational aggression and perceived popularity reciprocally influenced each other. Finally, social self-efficacy, leadership, cooperation, and peer sociability reciprocally influenced and were influenced by perceived popularity.

Sequence-specific binding of normal serum immunoglobulin M to exposed protein C-termini.

Both the timely clearance of degraded endogenous structures and the presence of secreted natural immunoglobulin M (IgM) are needed to avoid autoimmunity. These requirements may be causally related provided that natural IgM preferentially reacts with degraded antigens and, by activating complement, mediates their non-inflammatory clearance through complement receptors. We have previously shown that normal serum IgM reacts in vivo and in vitro with virtually all randomly generated C-terminal peptides displayed on T7 phage. The resultant multivalent IgM-peptide complexes activate complement and are detected by a loss of phage infectivity. A striking feature of these reactions is that different C-terminal peptides ( approximately 3-4 amino acids) specifically react with different 'C-terminal' IgM (C-IgM) antibodies. This suggests that degraded supramolecular structures, expressing elevated levels of identical C-termini as a result of proteolysis, denaturation and abnormal exposure of repetitive protein constituents, may be preferential targets of C-IgM-mediated complement activation in the physiological environment. The specificity of C-IgM-peptide reactions is much higher than one would expect, assuming that normal serum IgM mostly comprises polyspecific natural antibodies. However, it is possible that polyspecific IgM is not adequately registered by our 'functional' phage-inactivation assays. In this study, we resolve the issue of C-IgM specificity by directly characterizing the binding reactivity of normal serum IgM with phage-displayed C-terminal peptides.