Application of halophiles in UMFC (upflow microbial fuel cell) for the treatment of saline olive oil industrial wastewater coupled with eco-energy yield.

The olive oil industry faces a major problem of treating the wastewater with high organic content and safe disposal. Olive oil industrial wastewater (OOIWW) consists of highly toxic environmental pollutants with high salinity. Saline olive oil industrial wastewater was treated using halophilic consortium in UMFC (upflow microbial fuel cell) mobilized with carbon felt as electrode. Total and soluble COD (chemical oxygen demand), total suspended solids and phenol content removal were studied at different organic loads (0.56, 0.77, 1.05, 1.26, 1.52 and 1.8 gCOD/L). UMFC with OOIWW was optimized at 1.52 gCOD/L for high organic removal and corresponding electricity production. Total COD, soluble COD, TSS and phenol removal were 91%, 89%, 78%, and complete removal of phenol was accomplished at the optimized organic load (1.52 gCOD/L). Correspondingly, the maximum bioenergy yield was 784 mV with 439 mW/m2 (power density) and 560 mA/m2 (current density), respectively. The presence of prominent halophilic exo-electrogens such as Ochrobactrum, Marinobacter, Rhodococcus and Bacillus potently treated the OOIWW and exhibited high energy yield.

Biohydrogen production through energy efficient surfactant induced microwave pretreatment of macroalgae Ulva reticulata.

Macroalgal biomass being rich in carbohydrates, proteins and lipids in their cell wall has been considered as the most efficient organic rich sources for biofuel (biohydrogen) production. In this study, Pluronic P-123-induced microwave pretreatment was applied to disintegrate the marine macroalgae biomass, Ulva reticulata. Microwave disintegration was done by varying the treatment time and microwave power from 0 to 40 min and 0.09 KW to 0.63 KW. A maximum chemical oxygen demand (COD) solubilization of 22.33% was achieved at a microwave power and time duration of 0.36 kW and 15 min. Chemical (Pluronic P-123, a mild surfactant) was combined with optimum microwave disintegration conditions to increase the solubilization efficiency and this combined pretreatment achieved a maximum COD solubilization of 31.02% at 10 min pretreatment time and 0.06 g per g TS of Pluronic P-123 dosage. The present study indicated that combination of surfactant with microwave pretreatment substantially improves the COD solubilization with reduced pretreatment -time than mono microwave pretreatment. An optimal hydrogen yield of 98.37 mL was achieved through this combined pretreatment. The biohydrogen data was modelled with Gompertz model and the kinetic parameters derived through this model implies that the calculated adjusted R squared values for all the samples lies between 0.95 and 0.99. This shows that the model fitted biohydrogen experimental values accurately. In addition, Pluronic P-123-induced microwave pretreatment was regarded as energy efficient and cost effective than microwave pretreatment alone with net energy production and a greater energy ratio of 504.38 kWh/Ton macroalgae and 1.2 when compared to microwave pretreatment alone (-2975.6 kWh/Ton macroalgae and 0.5).

Biohydrogen production coupled with wastewater treatment using selected microalgae.

Microalgae such as Chlorella pyrenoidosa, Scenedesmus obliquus and Chlorella sorokiniana were cultivated in domestic wastewater for biohydrogen production. The comparison between the microalgae was executed based on biomass productions, biochemical yields and nutrient removal efficiencies. S. obliquus showed the possibility of growing in domestic wastewater reaching maximum biomass production, lipid, protein, carbohydrate yield and nutrient removal efficiency. All the three microalgae reached high biomass production of 0.90, 0.76 and, 0.71 g/L, respectively for S. obliquus, C. sorokiniana and C. pyrenoidosa. A higher protein content (35.76%) was obtained in S. obliquus. A similar pattern of lipid yield (25.34-26.23%) and carbohydrate yield (30.32-33.21%) was recorded in all selected microalgae. Chlorophyll-a content was higher in synthetic media-grown algae compared algae grown in wastewater. The maximum nutrient removal efficiencies achieved were 85.54% of nitrate by C. sorokiniana, 95.43% of nitrite by C. pyrenoidosa, ∼100% of ammonia and 89.34% of phosphorus by C. sorokiniana. An acid pre-treatment was applied to disintegrate the biomass of microalgae, followed by dark fermentation in batch mode to produce hydrogen. During fermentation process, polysaccharides, protein and lipids were consumed. Maximum hydrogen production of 45.50 ± 0.32 mLH2/gVS, 38.43 ± 0.42 mLH2/gVS and 34.83 ± 1.82 mL/H2/gVS was achieved by C. pyrenoidosa, S. obliquus and C. sorokiniana respectively. Overall, the results revealed the potential of microalgal cultivation in wastewater coupled with maximum biomass production lead to biohydrogen generation for environmental sustainability.

Antibacterial and antibiofilm activity of extracts from sponge-associated bacterial endophytes.

Sponges forms association with many bacteria that serve as sources of new bioactive compounds. The compounds are produced in response to environmental and nutritional conditions of the environment that enable them to protect their host from colonization. In this study, three sponge bacterial endophytes were isolated, identified, and subjected to solvent extraction processes. The identified bacteria are Bacillus amyloquifaciens, Bacillus paramycoides, and Enterobacter sp. The bacteria were cultured in two different fermentation media with varying nutritional composition for the extraction process. The extracts were evaluated for antibacterial and antibiofilm activity against microfouling bacteria and the chemical composition of each extract was analyzed via gas chromatography-mass spectrometry (GC-MS). The extract from the endophytes shows varying antibacterial and antibiofilm activity against the tested strains. Several compounds were detected from the extracts including some with known antibacterial/antibiofilm activity. The results showed variations in activity and secondary metabolite production between the extracts obtained under different nutritional composition of the media. In conclusion, this study indicated the role of nutrient composition in the activity and secondary metabolites production by bacteria associated with sponge Also, this study confirmed the role of sponge bacterial endophytes as producers of bioactive compounds with potential application as antifouling (AF) agents.

A novel conversion of marine macroalgal biomass to biofuel (biohydrogen) via calcium hypochlorite induced dispersion.

Environmental pollution due to the consumption of non-renewable energy lead the search for alternative eco-friendly renewable fuel. The study details the biohydrogen production efficiency by potential macroalgal (Ulva reticulata) biomass improved by a disperser combined with calcium hypochlorite pretreatment technology. Calcium hypochlorite was added to decrease the surface energy of the medium induced by sole disperser pretreatment. Optimum condition for algal disperser treatment was 10,000 rpm with 30 min as dispersion time. The specific energy spent for the disintegration of the macroalgal biomass was 1231.58 kJ/kg TS. COD solubilization rate of 11.79% was attained with mechanical pretreatment whereas increased to 20.23% with combined pretreatment. Combination of disperser with calcium hypochlorite significantly reduced the specific energy input spent to 500 kJ/kg TS. The amount of organic materials such as carbohydrates, proteins and lipids released were 680 mg/L, 283 mg/L and 136 mg/L respectively. Thus, the combinative pretreatment with disperser rotor speed (10,000 rpm) for pretreatment time (12 min) and calcium hypochlorite dosage (0.1 g/g) derived as optimum condition for effective solubilization of macroalgal biomass. Biohydrogen production potential was maximum in the macroalgae pretreated with both disperser and calcium hypochlorite recorded highest yield (54.6 mL H2/g COD) compared to the macroalgae pretreated with disperser alone (31.7 mL H2/g COD) and untreated macroalgae (11.5 mL H2/g COD).

Tannery wastewater treatment coupled with bioenergy production in upflow microbial fuel cell under saline condition.

Tannery industry handling huge amount of leather materials release immense amount of saline organic content wastewater. The present research was focused on the treatment of tannery industrial wastewater in UMFC (upflow microbial fuel cell) under saline condition (4%). The UMFC reactor was operated at different organic load (OL) such as 0.6, 1.2, 1.8 and 2.4 gCOD/L respectively. Total chemical oxygen demand (TCOD) removal at 0.6, 1.2, 1.8 gCOD/L was 87 ± 1.2%, 91 ± 1.2% and 93 ± 1.8% respectively. Soluble chemical oxygen demand (SCOD) removal in UMFC at 0.6, 1.2, 1.8 gCOD/L was 85 ± 0.6%, 88 ± 1.2% and 91 ± 1.8% respectively. Total suspended solids (TSS) removal was 49%, 78%, 81% at 0.6, 1.2, 1.8 gCOD/L OL in UMFC. Further, raise in OL to 2.4 gCOD/L showed decrease in TCOD, SCOD (80% and 72%) and TSS (60%) removal. Maximal power production of 854 mV with corresponding PD (power density) of 462 mW/m2 and CD (current density) of 523 mA/m2 was registered at 1.8 gCOD/L OL in UMFC. Increase in OL to 2.4 gCOD/L revealed decline in energy production to 810 mV with PD (385 mW/m2) and CD (438 mA/m2) in UMFC. Maximal bioaccumulation of chromium (95%) was recorded at 1.8 gCOD/L OL. Among different OL used 1.8 gCOD/L OL was optimum for the treatment of tannery wastewater and energy production. Bacterial community analysis in anode of UMFC revealed the dominance of promising electrogenic halophilic strains such as Ochrobactrum, Marinobacter, Rhodococcus and Bacillus in all the OL. Thus, the research clearly revealed the efficacy of halophilic consortium to treat the saline tannery wastewater coupled with bioenergy production in UMFC.

Macroalgae (Ulva reticulata) derived biohydrogen recovery through mild surfactant induced energy and cost efficient dispersion pretreatment technology.

Currently identification of alternate fuel is the key area of research under progress to overcome the depletion of fossil fuels, meet the domestic and industrial requirements. Generation of hydrogen, which is a clean fuel gas can solve various environmental related problems. Extensive research is being carried out to increase production of hydrogen through different substrates. This study aims to increase the production of hydrogen from Ulva reticulata (a macroalgal biomass). Initially, the biomass is pretreated mechanically with disperser and a biosurfactant, namely rhamnolipid in order to increase the solubilization of the biomass. The rate of COD liquefaction increased from 14% to 25% with the addition of biosurfactant to the macroalgal biomass, which is further treated mechanically using a disperser. The disperser rotor speed of 12,000 rpm and the specific energy input of 1175 kJ/kg TS (Total Solids) with the disintegration time of 30 min and biosurfactant dosage of 0.075 g/g TS were considered as the optimum parameters for the effective liquefaction of the macroalgal biomass. Approximately 3500 mg/L of the biopolymers were released after the combinative pretreatment (using disperser and biosurfactant). About 80 mL biohydrogen/g COD (Chemical Oxygen Demand) was generated when the biomass was pretreated with both the disperser and biosurfactant while the biomass pretreated with the disperser alone generated just 30 mL biohydrogen/g COD and the untreated biomass generated 5 mL biohydrogen/g COD. Thus, it can be concluded that Ulva reticulata can be utilized effectively to generate biohydrogen.

Bioaugmentation of electrogenic halophiles in the treatment of pharmaceutical industrial wastewater and energy production in microbial fuel cell under saline condition.

Pharmaceutical wastewater with different toxic recalcitrant materials and high salinity requires a novel treatment technology before released into the environment. The present research details the treatment of pharmaceutical wastewater along with energy production using bioaugmentation of halophilic consortium in air cathode microbial fuel cell (ACMFC) under saline condition (4%). Organic load (OL) varied from 1.04 to 3.51 gCOD/L was studied in ACMFC. TCOD (Total Chemical Oxygen Demand) removal exhibited 65%, 72%, 84% and 89% at 1.04, 1.52, 2.01 and 2.52 gCOD/L OL respectively. SCOD (Soluble Chemical Oxygen Demand) removal of 60%, 66%, 76% and 82% was recorded during the operation of identical OL (1.04-2.52 gCOD/L). Prominent TCOD (92%), SCOD (90%), TSS (Total Suspended Solids) removal of 73% was attained at 3.02 gCOD/L OL with corresponding energy production of 896 mV (Current density (CD) - 554 mA/m2, Power density (PD)-505 mW/m2). CE (Columbic Efficiency) was 43%, 38%, 33%, 30%, 28% and 22% at different OL ranged between 1.04 and 3.51 gCOD/L. Increase in OL to 3.51 gCOD/L revealed decrement in TCOD (68%), SCOD (62%), TSS (52%) removal and energy production (CD-234 mA/m2, PD-165 mW/m2). Complete removal of phenol was accomplished at different OL in 6 (1.04, 1.52 gCOD/L) and 8 (2.01, 2.52 and 3.02 gCOD/L) days respectively. Ochrobactrum, Marinobacter, Bacillus and Rhodococcus were the dominant halophilic electrogenic strain in ACMFC at different OL.

Biofuel production from Macroalgae: present scenario and future scope.

The current fossil fuel reserves are not sufficient to meet the increasing demand and very soon will become exhausted. Pollution, global warming, and inflated oil prices have led the quest for renewable energy sources. Macroalgae (green, brown, and red marine seaweed) is gaining popularity as a viable and promising renewable source for biofuels production. Numerous researches have been conducted to access the potential of macroalgae for generating diverse bioproducts such as biofuels. The existence of components such as carbohydrates and lipids, and the lack or deficiency of lignin, create macroalgae an enviable feedstock for biofuels generation. This review briefly covers the potential macroalgal species promoting the production of biofuels and their cultivation methods. It also illustrates the biofuel generation pathway and its efficiency along with the recent techniques to accelerate the product yield. In addition, the current analysis focuses on a cost-effective sustainable generation of biofuel along with commercialization and scaleup.

Treatment of fish market wastewater and energy production using halophiles in air cathode microbial fuel cell.

The present study is aimed to treat the fish market wastewater coupled with electricity production using halophiles in microbial fuel cell (MFC) technology under saline condition (4.6%). Halophilic consortium obtained from desalination plant brine water was used in the lab scale air cathode microbial fuel cell (ACMFC) reactor equipped with carbon brush and carbon cloth as anode and cathode. ACMFC (260 mL capacity) was operated with fish market saline wastewater at different organic load (OL) from 0.41 to 2.01 g COD/L with 20 day HRT (Hydraulic Retention Time). Total chemical oxygen demand (TCOD) removal at OL 0.41, 0.82 and 1.21 g COD/L was 68%, 77% and 84% in ACMFC. Correspondingly, soluble chemical oxygen demand (SCOD) removal was 63%, 74% and 81% respectively. The optimized OL for the treatment of fish market wastewater was 1.62 g COD/L, where the TCOD (90%), SCOD (88%), TSS (Total Suspended Solids) removal of 71% coupled with power generation of 902 mV (Power density 420 mW/m2, Current density 550 mA/m2) was recorded. Columbic efficiency at OL 0.41 g COD/L was 56% and declined at OL 0.82, 1.21, 1.62 and 2.01 g COD/L to 48%, 39%, 29% and 17%. Increment in OL to 2.01 g COD/L revealed decrease in TCOD (64%), SCOD (60%), TSS (45%) removal and energy production. The bacterial strains present in the halophilic consortium were Ochrobactrum, Marinobacter, Bacillus, Rhodococcus, Flavobacterium, Alicyclobacillus, Pseudomonas, Martelella, Stenotrophomonas, Xanthobacter, and Microbacterium. High dominance of Ochrobactrum, Marinobacter and Bacillus was observed at optimized OL of 1.62 g COD/L in ACMFC. Further research on pilot scale MFC lead the way to technology transfer for the treatment of wastewater with corresponding energy production in industrial sector.

Application of integrated extremophilic (halo-alkalo-thermophilic) bacterial consortium in the degradation of petroleum hydrocarbons and treatment of petroleum refinery wastewater under extreme condition.

Degradation of petroleum hydrocarbon under extreme conditions such as high salinity, temperature and pH was difficult due to unavailability of potential bacterial strains. The present study details the efficiency of extremophilic bacterial consortium in biodegradation of different petroleum hydrocarbons and treatment of petroleum refinery wastewater under extreme condition. Extreme condition for the degradation of petroleum hydrocarbons was optimized at 8% salinity, pH-10 and temperature-60 °C. The consortium recorded complete degradation of low molecular weight (LMW) petroleum hydrocarbons (200 ppm) such as anthracene, phenanthrene, fluorene and naphthalene in 8 days under optimized extreme condition. High molecular weight (HMW) hydrocarbons such as pyrene (100 ppm), benzo(e)pyrene (20 ppm), benzo(k)fluoranthene (20 ppm) and benzo(a)pyrene (20 ppm), revealed 93%, 60%, 55% and 51% degradation by the extremophilic consortium under optimized extreme condition. The extremophilic consortium mineralized fluorene (61%) at high saline condition up to 24%. Addition of yeast extract potently accelerated the biodegradation under extreme condition. Treatment of petroleum refinery wastewater in continuous stirred tank reactor recorded 92% COD removal with complete removal of LMW hydrocarbons in 16 days and 91% of HMW hydrocarbons in 32 days under extreme condition. The hydrocarbons degrading extremophilic consortium possessed Ochrobactrum, Bacillus, Marinobacter, Pseudomonas, Martelella, Stenotrophomonas and Rhodococcus.

Deriving electricity from dye processing wastewater using single chamber microbial fuel cell with carbon brush anode and platinum nano coated air cathode.

Single chamber air cathode microbial fuel cell (MFC) is a promising and sustainable technology to generate electricity. In the present study, the potential of air cathode MFC treating dye processing wastewater was investigated at various organic loads with interest focused on power densities, organic removal and coulombic efficiencies. The highest power density of about 515 mW/m2 (6.03 W/m3) with 56% of coulombic efficiency was procured at 1.0 (g COD/L) organic load. The high potency of TCOD (total chemical oxygen demand), SCOD (soluble chemical oxygen demand) and TSS (Total Suspended Solids) removal of about 85%, 73% and 68% respectively was achieved at the organic load of 1.0 (g COD/L). The bacterial strains in anode region at the initial stage of MFC operation were reported to be responsible for potential organic removal. The bacterial strains in air cathode MFC were identified as Paenibacillus sp. strain JRA1 (MH27077), Pseudomonas sp. strain JRA2 (MH27078), Ochrobactrum sp. strain JRA3 (MH27079), Sphingobacterium sp. strain JRA4 (MH27080), Stenotrophomonas sp. strain JRA5 (MH27081), Bacillus sp. strain JRA6 (MH27082) and Clostridium sp. strain JRA7 (MH27083) using phylogenetic analysis. After 60 days of air cathode MFC operation, the bacterial community in biofilm samples was dominated by Bacillus, Ochrobactrum and Pseudomonas (20-22%). The biofilm sample collected from the carbon brush consisted of Bacillus (33%), Ochrobactrum (30%), Pseudomonas (28%), Clostridium (6%) and Stenotrophomonas (3%). The present study revealed the treatment efficiency of dye processing wastewater along with power generation in single chambered air cathode MFC.

Degradation of petroleum hydrocarbons and treatment of refinery wastewater under saline condition by a halophilic bacterial consortium enriched from marine environment (Red Sea), Jeddah, Saudi Arabia.

A halophilic bacterial consortium was enriched from Red Sea saline water and sediment samples collected from Abhor, Jeddah, Saudi Arabia. The consortium potentially degraded different low (above 90% for phenanthrene and fluorene) and high (69 ± 1.4 and 56 ± 1.8% at 50 and 100 mg/L of pyrene) molecular weight polycyclic aromatic hydrocarbons (PAHs) at different concentrations under saline condition (40 g/L NaCl concentration). The cell hydrophobicity (91° ± 1°) and biosurfactant production (30 mN/m) confirmed potential bacterial cell interaction with PAHs to facilitate biodegradation process. Co-metabolic study with phenanthrene as co-substrate during pyrene degradation recorded 90% degradation in 12 days. The consortium in continuous stirred tank reactor with petroleum refinery wastewater showed complete and 90% degradation of low and high molecular weight PAHs, respectively. The reactor study also revealed 94 ± 1.8% chemical oxygen demand removal by the halophilic consortium under saline condition (40 g/L NaCl concentration). The halophilic bacterial strains present in the consortium were identified as Ochrobactrum halosaudis strain CEES1 (KX377976), Stenotrophomonas maltophilia strain CEES2 (KX377977), Achromobacter xylosoxidans strain CEES3 (KX377978) and Mesorhizobium halosaudis strain CEES4 (KX377979). Thus, the promising halophilic consortium was highly recommended to be employed in petroleum saline wastewater treatment process.

Biodegradation of low and high molecular weight hydrocarbons in petroleum refinery wastewater by a thermophilic bacterial consortium.

Clean-up of contaminated wastewater remains to be a major challenge in petroleum refinery. Here, we describe the capacity of a bacterial consortium enriched from crude oil drilling site in Al-Khobar, Saudi Arabia, to utilize polycyclic aromatic hydrocarbons (PAHs) as sole carbon source at 60°C. The consortium reduced low molecular weight (LMW; naphthalene, phenanthrene, fluorene and anthracene) and high molecular weight (HMW; pyrene, benzo(e)pyrene and benzo(k)fluoranthene) PAH loads of up to 1.5 g/L with removal efficiencies of 90% and 80% within 10 days. PAH biodegradation was verified by the presence of PAH metabolites and evolution of carbon dioxide (90 ± 3%). Biodegradation led to a reduction of the surface tension to 34 ± 1 mN/m thus suggesting biosurfactant production by the consortium. Phylogenetic analysis of the consortium revealed the presence of the thermophilic PAH degrader Pseudomonas aeruginosa strain CEES1 (KU664514) and Bacillus thermosaudia (KU664515) strain CEES2. The consortium was further found to treat petroleum wastewater in continuous stirred tank reactor with 96 ± 2% chemical oxygen demand removal and complete PAH degradation in 24 days.