[May GM-CSF influence the ability to fertilize oocytes?]. / Czy GM-CSF moze wplywac na zdolnosc do zaplodnienia oocytów?

The aim of this work was to evaluate the concentration of granulocyte-macrophage colony stimulating factor (GM-CSF), IL-1 beta IL-6 and TNF alpha in follicular fluid obtained from mature oocytes during in vitro fertilization scheme and to determine a correlation between GM-CSF concentration and the analyzed cytokines. Material consisted of 36 samples of follicular fluid which were divided into two groups: the first one--17 samples of follicular fluid obtained from follicles with successful oocyte fertilization and cleavage and the second one--19 samples of follicular fluid with unsuccessful fertilization. The concentration of GM-CSF as well as IL-6, IL-1 beta and TNF alpha in follicular fluid was evaluated by using the ELISA immunosorbent assays. The mean concentration of GM-CSF, IL-6, IL-1 beta was similar in both groups, however, the frequency of occurrence of these parameters as well as coexistence of GM-CSF with analyzed cytokines were higher in the second group i.e. in the follicular fluid with unsuccessful fertilization. We found a positive correlation between concentration of GM-CSF and IL-6 in follicular fluid of all samples. The authors suggested that activation of macrophages to an increased release of GM-CSF during disturbance of oocyte's maturation might implicate a relation between immune and endocrine systems with cytokine mediation.

[Examination of 17 beta-estradiol and progesterone levels in peritoneal fluid and blood serum of women with endometriosis]. / Badanie stezenia 17 beta-estradiolu i progesteronu w plynie otrzewnowym i surowicy krwi kobiet z endometrioza.

Authors have presented and assessment of estradiol and progesterone levels in peritoneal fluid and blood serum in women with endometriosis. Peritoneal fluid was collected during laparoscopy performed in luteal phase of the cycle. In this cycle ovulation was controlled in all women. An ovulation was confirmed ultrasonographically and laparoscopically in 45% of women with endometriosis and in 80% of that without the illness. Progesterone concentration in peritoneal fluid in women with endometriosis was significantly lower to the control (p < 0.01).

[Evaluation of peritoneal fluid in women with endometriosis]. / Ocena plynu otrzewnowego u kobiet z endometrioza.

An analysis of the peritoneal fluid in women with endometriosis was undertaken. Peritoneal fluid was collected during laparoscopy from the Douglas pouch. The quantity, color of the fluid as well as macrophages number, concentration and activity were assessed. Conclusion is that no one of the listed parameters of peritoneal fluid could be considered as an endometriosis marker.