Continuous monitoring of noise levels in the Gulf of Catania (Ionian Sea). Study of correlation with ship traffic.

Acoustic noise levels were measured in the Gulf of Catania (Ionian Sea) from July 2012 to May 2013 by a low frequency (<1000Hz) hydrophone, installed on board the NEMO-SN1 multidisciplinary observatory. NEMO-SN1 is a cabled node of EMSO-ERIC, which was deployed at a water depth of 2100m, 25km off Catania. The study area is characterized by the proximity of mid-size harbors and shipping lanes. Measured noise levels were correlated with the passage of ships tracked with a dedicated AIS antenna. Noise power was measured in the frequency range between 10Hz and 1000Hz. Experimental data were compared with the results of a fast numerical model based on AIS data to evaluate the contribution of shipping noise in six consecutive 1/3 octave frequency bands, including the 1/3 octave frequency bands centered at 63Hz and 125Hz, indicated by the Marine Strategy Framework Directive (2008/56/EC).

["Assessment of indoor school environment and identification of measures to protect the respiratory health of school children and adolescents" in a sample of schools in Milan]. / Valutazione dei fattori di rischio indoor in ambiente scolastico e definizione delle misure per la tutela della salute respiratoria degli scolari e degli adolescenti" in un campione di scuole di Milano.

The management of indoor air quality in schools needs special attention because it has a strong impact on respiratory health of children with effects also on performance and social development. In Italy a prevention program for indoor environments is provided in the "Guidelines for the prevention of indoor risk factors for allergies and asthma in the school", developed by the Ministry of Health (G.U n. 9 del 13.01.11). In this context, the Ministry of Health has promoted the "Indoor school" project (CCM2010). The main objective of the project is the implementation of these guidelines. In this paper we report the results of the first phase of the project which assessed the knowledge of school principals on issues related to IAQ and building characteristics of the school.

[Radon risk evaluation in a hospital context]. / Valutazione del rischio da Radon in una realtà ospedaliera.

Italian legislation imposes radon concentration in occupational, underground premises as to not exceed the annual action level of 500 Bq/m3. A survey in a hospital of Milan founded concentrations never exceeding the action level, distribute around the median concentration of 16 Bq/m3, ranging between 6 e 214 Bq/m3 (Cmean = 38 +/- 50 Bq/m3). However, a pavilion, characterized by higher levels (C = 103 divided by 214 Bq/m3), was better studied by short-term monitoring, during summer and winter, confirming concentrations not exceeding legislative level, even if not negligible. Results underlines the importance of a monitoring strategy as punctual as possible. Moreover, shortterm measurements can represent a valid tool for premises screening in radon monitoring.

[Evolution of industrial toxicology toward vanishing doses and the human genome]. / Evoluzione della tossicologia industriale tra dosi effimere e genoma umano.

BACKGROUND: This article aims to discuss the influence that the application of the recent discoveries in genomics will have on the theory and practice of industrial toxicology in developed post-industrial countries. It is stressed that the recent advances in toxicogenomics can be integrated into the existing wealth of knowledge on the toxic properties of industrial chemicals to improve the efficacy of prevention of toxicological risk. METHODS AND RESULTS: The understanding of the biochemical and physiological mechanisms underlying susceptibility or resistance to the toxic effects of industrial xenobiotics, and in particular to carcinogens, allows us to split the epidemiologically derived relationship linking the frequency of disease in the exposed population to the level of workplace contamination into a set of sequential sub-relationships linking: a) the exposure level to that of workplace contamination; b) the internal dose to the exposure level; c) the biological effect (e.g., measured through biochemical markers of early effect) to the internal dose; d) the frequency of disease to that of observation of early biochemical effects. Each of the cited relationships is affected by a degree of uncertainty due to the variability of biological response among the examined individuals, which in turn requires a definition of the statistical limits for the association functions between the variables. As a consequence, the possibility of investigating the individual biochemical and physiological steps in the causal mechanism that links toxic exposure to disease does not necessarily lead to an increase in the information potential of biological monitoring, since the uncertainty due to inter-individual variability is amplified through the sequence of causal relationships to the point that the data from biological monitoring become valueless with regard to the prediction of the frequency or probability of disease. This is particularly true when exposure to 'low doses' is investigated, as is now increasingly frequent in post-industrial developed countries, where workplace contamination is now greatly reduced to levels which may be borderline with those in the general environment. Thus at the low-dose end of the range of contamination and exposure values there is an area where, for statistical reasons consequent to the heterogeneity of examined populations, a quantitative prediction of internal exposure due to environmental contamination, of biological adverse effects due to exposure levels and of frequency of disease due to the extent or frequency of biological effects is no longer reliably possible. This in turn impairs the preventive efficacy of biological monitoring. CONCLUSIONS: A closer integration between industrial toxicology and state-of-the-art molecular genetics derived from the recent sequencing of the human genome is the way to overcome the limitations described. In particular, the individual subjects in the examined populations can be classified with regard to some genetically controlled characters relevant to the biotransformation of xenobiotics and to DNA repair and the statistical analysis of data can be performed on more homogeneous subpopulations, in order to decrease inter-individual variability of biochemical and physiological response. This in turn increases the predictive power of the biological markers, both of dose and effect, and improves the efficacy of prevention, e.g., by highlighting oversensitive subpopulations or lifestyles which can increase the risk of occupational and environmental disease.

Headspace solid-phase microextraction for the determination of benzene, toluene, ethylbenzene and xylenes in urine.

A method for the determination of benzene, toluene, ethylbenzene and xylenes (BTEX) in urine of people exposed to these airborne pollutants present in the living environment, has been described. Solid-phase microextraction has been used for sampling BTEX from the headspace of urine and gas chromatography-mass spectrometry has been applied for the selective analysis of chemicals. The method has the following features: small volume of urine (2 ml) needed, linearity in the range of interest (from the limit of detection up to 5000 ng/l) with coefficient of correlation > or =0.998, limit of detection in the range 12-34 ng/l, good repeatability (coefficient of variation 2-7%), high specificity. The stability of the urine sample during storage (-20 degrees C) was evaluated: BTEX remained stable for up to 2 months. The assay has been successfully applied to the biological monitoring of two subjects environmentally exposed to airborne BTEX in an urban area.

[Bizarre gastric leiomyoblastoma. A case report and review of the literature]. / Leiomioblastoma gastrico bizzarro. Analisi di un caso e revisione della letteratura.

Gastric leiomyoblastoma of Martin's myoid tumour or Stout's smooth muscle cell bizarre tumour is a rare benign mesenchymal neoplasia, that may become malignant. A personal clinical case is reported and the most important clinical and anatomopathological aspects are reviewed, stress being laid on the importance of identifying the macroscopic and histological features that can point with certainty to malignity and so influence the choice of surgery.

[Biological monitoring of exposure to solvents: a method for the gas-chromatographic determination of aromatic hydrocarbons in the blood and urine]. / Monitoraggio biologico dell'esposizione a solventi: metodo per la determinazione gascromatografica degli idrocarburi aromatici nel sangue e nell'urina.

A gas chromatographic procedure with dynamic head-space purge and trap preconcentration (HSGC) and FID detection for blood and urinary benzene, toluene, ethylbenzene and xylenes (BTEX) determination at low level exposure is described. Critical steps (sample collection, calibration, HSGC conditions, contamination control) are discussed. The calibration curve is linear in the range 50 ng/l-500 micrograms/l; the calculated detection limit is 50 ng/l for all the considered aromatic hydrocarbons (AH) both in blood and urine; the within-day precision, calculated as variation coefficient (CV) at 400 ng/l and 40 micrograms/l (n = 6) was respectively CV = 13% and CV = 6% for all the studied analytes. The recovery rate was in the range 29-70%, depending on the hydrocarbon and matrix (blood or urine) considered. The procedure was applied to the biological monitoring of 151 workers occupationally or environmentally exposed to BTEX. Occupationally exposed subjects showed blood AH levels of 2-4 order of magnitude higher than environmentally exposed subjects. In white-collar workers exposed to BTEX urban pollution a significant difference in blood and urine levels of AH was observed between nonsmokers and smokers. Nonsmokers showed blood AH median values of respectively benzene = 241 ng/l, toluene = 759 ng/l, ethylbenzene = 140 ng/l, xylenes = 604 ng/l. Significatively higher BTEX blood values were observed in smokers after a median consumption of 5 cigarettes in 5 hours; observed median values were respectively: benzene = 365 ng/l toluene = 1327 ng/l, ethylbenzene = 233 ng/l, xylenes = 794 ng/l.

Transitional papillary cell carcinoma of the ureter: a histological and ultrastructural study.

We report a case of transitional papillary carcinoma of the distal left ureter. The histological and ultrastructural features were seen and compared by SEM and TEM. Observations confirm the data of literature that the deep cells of neoplasm are a normal morphological finding while the superficial cells do not have the characteristics present in normal transitional cells. In addition, SEM shows small groups or single cells with "pleomorphic microvilli" on the cell surface. These, in ureter, confirm the studies of many authors who have observed these abnormalities in carcinomas of several organs like breast, colon, liver and mesothelium.

[An anatomicoclinical case of lipoma of the sigmoid]. / Su un caso anatomo-clinico di lipoma del sigma.

The authors describe a case of a submucosal lipoma of the large intestine. Family, physiological, and remote anamnesis of the patient was negative to inflammatory or neoplastic intestinal diseases. The symptomatology was not characteristic like the several cases reported in the literature, by abdominal cramps, subocclusion or total occlusion crisis and rectal bleeding. The patient presented with a symptomatology characterized by altered bowel habit, and evacuations of blood-stained feces. The clinical diagnosis was difficult for the aspecific symptoms referred. A barium enema and colonoscopy examination showed a suspected benign neoformation localized within the sigmoid colon. The patient underwent surgery and the following histologic exam exhibited a submucosal lipoma with an atrophied superficial epithelium replaced by necrotic and granular tissue, marked architectural disarray of glandular crypts and intensive inflammatory infiltrate of tunica mucosa.

[Ascaridiasis: a rare cause of recurrent abdominal pain. Report of a clinical case documented by ultrastructural study of the intestinal mucosa]. / L'ascaridiasi: una rara causa di dolori addominali ricorrenti. Presentazione di un caso clinico corredato dallo studio ultrastrutturale della mucosa intestinale.

The case of a six-year-old girl suffering from recurrent abdominal pain is reported. On the basis of the laboratory tests and a number of other clinical investigations, the diagnosis of ascaridiasis was made. At scanning electron microscopy the ultrastructural study of the bioptic fragments obtained during endoscopy showed peculiar lesions of the gastric and duodenal mucosa. We speculate that this unusual picture may be due to the ascaris. These lesions, described for the first time in the literature to our knowledge, were represented by the loss of the apical portion of some cells. Differential diagnosis of recurrent abdominal pain is discussed.

[Asymptomatic peritoneal endometriosis following cesarean section]. / Endometriosi peritoneale asintomatica dopo taglio cesareo.

Endometriotic peritoneal implants of small dimensions were discovered in five patients undergoing laparoscopic examination for diseases unrelated to endometriosis. All patients were asymptomatic for endometriosis and each had undergone at least one previous cesarean section. Biopsies taken were studied by light microscopy which evidenced variably predominating epithelial or stromal components of lesions Perls' method was used to evidence tissue ferric iron resulting from long-standing micro-haemorrhagic foci. From this study, isolated, asymptomatic peritoneal implants of endometrium are suspected to occur frequently after cesarean section although the progression of this condition to a symptomatic state is considered infrequent and independent of specific histologic aspects of lesions.

[Dissecting pseudomembranous celiocytic vaginitis caused by HPV in a HIV positive patient]. / Vaginite dissecante pseudomembranosa coilocitosica da HPV in soggetto HIV positivo.

The Authors describe a case of dessicant pseudomembranous vaginitis caused by HPV types 6, 11, 16 and 18 in a 44-year-old HIV seropositive woman. A material mixed with blood, simulating a spontaneous abortion because of its quantity and consistency, was ejected vaginally. A spontaneous abortion was excluded on the basis of a pelvic echographic exam and on the beta-HCG plasma level. Histologic examination of the ejected material demonstrated an epithelium of the vaginal mucosa in which was found a marked and extensive koilocytosis of intermedial and parabasal layers. The vaginal mucosa in this patient differed from the commonly observed koilocytosis and condylomatous proliferation histological aspects seen in vaginal HPV lesions. In the context of the epithelium, by means of confluence of voluminous koilocytotic elements, pseudocystic lacunar spaces of varying dimensions definable as "koilocytotic pseudocysts" were observed. Within the vaginal epithelium, along a line of reduced resistance, a massive and extensive dessication resulted in a laminar detachment of the overlying epithelial stratum. Regarding the association between HIV and HPV infection, it is known that seropositive patients with immunodeficiencies are at high risk of infection by a variety of pathogens and, among these, different types of HPV. The viral association in this case undoubtedly promoted the extension and seriousness of the lesion and its exceptional complication.

[A case of multiple intestinal perforations due to TBC in a female patient with HIV-1 infection]. / Su di un caso di perforazioni intestinali multiple da TBC in una paziente con infezione da HIV-1.

The authors report a case of HIV-1 infection, primary miliary tuberculosis of the lung and secondary intestinal tuberculosis complicated with multiple intestinal perforations. The surgical emergency therapy, supported by specific antitubercular drugs, antibiotics, and total parenteral nutrition, consisted in suturing seven perforative sites of the small and large intestine. The Authors stress the possibility that in the future tubercular complications up till now considered atypical may become more and more frequent because of the HIV-1 infection association.