Acción antimicrobiana in vitro de distintas medicaciones sobre Entercoccus faecalis y Actinomyces israeli / In vitro antimicrobila effects of different root canal medicaments on Enterococcus faecalis and Actinomyces israelii

Objetivo: Se comparó in vitro la acción antimicrobiana de diversas medicaciones intaconducto frente a Enterococcus faecalis y Actinomyces israelli. Material y métodos: para evaluar las zonas d inhibición microbiana se utilizó el test de difusión en agar frente a diversas pastas, incluyendo una pasta con base de metronidazol (Grinazole®), una pasta con base de dexametasona, tiretrocina, poimiana y neoicina (Septomixine forte ®) y otra de hidróxido de calcio (Calcipulpe®) y paroclofenol alcanforado (KRI-3®), frente a E. faecalis y A. israelii. Resultados: a acción inhibitoria frente E. faecalis fue significativamente mayor (p<0,05) con Septomixine (30,7 mm) seguido de KRI-3® (22,5 mm), Calcipulpe ®(16,6 mm) y Grinazole ® (11,4 mm). La inhibidción microbiana frente a E. israelii fue significativamente mejor cn Septomixine forte® (p<0,05) (40,1 mm) segudo de KRI-3® (36,6 mm), Grinazole ® (7,6 mm) y Calcipulpe ® (6mm). Conclusión: Septomixine forte ® fue la pasta antibiótica más efectiva frente a E. faecalis y A. israelii comunmente hallados en las infecciones endodóncicas (AU)

Objective: To compare in vitro antimicrobial effect of differnet root canal medicaments on Enterococcus faecalis and Actinomyces israelii. Methology: The agar diffusión test was used to assess the bacterial growth nhitition zones achieved with different antibiotic pastes, including a metronidazole containg paste (Grinazole ®) a combintion of dexamethasone, tyrothricin and both polymyxin and neomycin sulfate (Septomixine forte ®) , calcium hydroxide (Calcipulpe ®), and camphorated paramonochloropheno (Kri-3®) aginst E. faecalis and A israelli. Results: Antibacterial nhibition against E. faecalis was significantly (P<0.05) greater with Septomixine forte® (30,7 mm) followed by Kri-3® (22,5 mm), Calcipulpe ® (16,6 mm), and Grinazole ® (11,4mm). Bacterial inhibition against A. israelii was also significantly greater (P<0,05) with Septomixine forte ® (40,1mm) followed by Kri-3® (36,6 mm), Grinazole ® (7,6 mm), and Calcipulpe ® (6 mm). Conclusión: Septomixine forte ® was the most effective antibiotic paste against E. faecalis and A. israelii comonly found in endodontic infections (AU)

El surco de desarrollo radicular como causa de fracaso endodóncico: a propósito de un caso / Root development sulcus as a cause for endodontic treatment failure: a case report

El surco de desarrollo radicular es una anomalía dentaria de origen embriológico presente en el 8,5% de la población que se localiza principalmente en la cara palatina de los incisivos laterales superiores. Este surco se origina en la fosa central y atravesando el cíngulo se dirige hacia el ápice, siendo su longitud variable. A través de él, los microorganismos penetran en el ligamento periodontal, causando una destrucción ósea localizada. Clínicamente los dientes afectados por esta anomalía pueden ser asintomáticos o bien presentar patología periodontal, pulpitis o necrosis pulpar por afectación secundaria de la pulpa. Presentamos el caso de una paciente que acudió a nuestro Servicio para realizarse la cirugía periapical del 1.2. En el examen físico se detectó y se exploró el surco. Asimismo, también se observó que el tratamiento de conductos era correcto y no existía patología a nivel periapical. Se decidió efectuar una cirugía exploratoria donde se comprobó la presencia de un surco de desarrollo radicular. El tratamiento final efectuado fue la exodoncia. El pronóstico de estos dientes es incierto, dependiendo de la profundidad y de la extensión apical del surco radicular, así como de la higiene bucal del paciente. Diversos autores han intentado el tratamiento mediante la eliminación del tejido de granulación, odontoplastia y la aplicación de ácido cítrico, el sellado del surco con amalgama, o el uso de hidroxiapatita y técnicas de regeneración tisular guiada pero en la mayoría de los casos el tratamiento de elección continúa siendo la exodoncia

The radicular growth groove is an embriologic dental anomaly that affects the 8,5 % of the population. It is located in the palatal face of the maxillary lateral incisors. This groove begins in the central fossa, runs through the cingulum and arrives to the apex, having a variable length. The microorganisms can penetrate to the ligament periodontal through the groove, causing local bone destruction. These teeth can be asymptomatic, can present periodontal patology, pulpitis or pulp necrosis due to the secondary affectation of the pulp. We present the case of a patient that came to our clinic to do the periapical surgery of the right maxillary lateral incisor. We detected and explored the groove in the physic exam. We observed that the endodontic treatment was correct too and it didn't exist periapical patology. We decided to do an exploratory surgery where we saw the radicular growth groove. The final treatment was the extraction of the tooth. The prognosis of these teeth is uncertain and it depends on the depth and length of the radicular growth groove and on the oral hygiene of the patient. Some authors have attempted the treatment removing the inflamatory tissue, doing an odontoplasty and applying citric acid, sealing the groove with amalgam or using hidroxiapatite and guided tissular regeneration technics, but in most of the cases the extraction of the tooth is the election treatment

Antimicrobial activity of seven root canal sealers. Results of agar diffusion and agar dilution tests.

A comparative study of the antimicrobial action of seven root canal sealers: Traitement Spad, Endométhasone, N2 Universal, Diaket-A, AH26 with silver, Tubli Seal, and Sealapex was done with 120 strains of Staphylococcus aureus. Two antimicrobial susceptibility tests were used: the agar dilution test and the agar diffusion test. The Diaket-A and Traitement Spad sealer cements showed the highest efficiency in the dilution test, whereas Diaket-A was in fourth place in the diffusion test, only better than the antimicrobial activity of the Tubli Seal and Sealapex sealers.

[Behavior of different strains of Staphylococcus aureus against root canal filling cements]. / Estudio del comportamiento de diferentes cepas de Staphylococcus aureus frente a los cementos de obturación de conductos radiculares.

The mean goal of this study is the determination of the conduct of 120 strains of Staphylococcus aureus against seven root canal sealers: Traitement Spad, Endométhasone, N2 Universal, AH26 with silver, Diaket-A, Tubli Seal and Sealapex. The agar diffusion test was employed in the determination of its bacterial growth inhibition. The results obtained have demonstrated values very different between the tested strains. Therefore we recommended to employ strains with reference in the investigation of the bacterial growth inhibition in order to repeat equal experimentation conditions.

Bacterial growth inhibition produced by root canal sealer cements with a calcium hydroxide base.

Inhibition of growth of six bacterial strains produced by two root canal sealers with a calcium hydroxide base, CRCS and Sealapex sealers, is studied. The results are compared with those obtained with two zinc oxide eugenol sealers and one epoxy resin. The inhibition produced with the calcium hydroxide sealers is similar to that obtained with the other sealers. The component of paraformaldehyde in a sealer increases the inhibition significantly.