ABSTRACT
To study the effects of loxosceles laeta venom on red blood cells and the possible factors involved in hemolysis during arachnidism, in vitro models were designed to measure the role of loxosceles venom, calcium, complement and antibodies in the mechanism of red blood cell destruction. The degree of basal hemolysis was measured in a 5% suspension of group O, Rh (+) red blood cells in pH 7.4 buffer. In a similar suspension spider venom was added in amounts equivalent to one venom gland. After 72 hours of incubation, basal hemolysis was 5.59 +/- 2.04% which increased to 26.01 +/- 7.9% adding venom (p < 0.001). Adding calcium to the incubation medium, hemolysis increased to 88.5 +/- 7.16% (p < 0.001). Incubating red blood cells with control human serum and venom, hemolysis was 14.58 +/- 2.42%, which decreased significantly to 6.85 +/- 3.35% when serum was heat inactivated; this demonstrates an effect of the presence of complement. We did not find antivenom antibody production in patients with arachnidism 10, 15 or 30 days after the spider bite. It is thus demonstrated that loxosceles laeta venom has a direct lytic action on red blood cells that is calcium and complement dependent and is not mediated by antibodies or other substance.
Subject(s)
Erythrocytes/drug effects , Hemolysis , Spider Venoms/pharmacology , Animals , Humans , In Vitro Techniques , Spider Bites/blood , Spider Bites/immunology , Spider Bites/therapy , Time FactorsABSTRACT
Se estudian algunas proteinas plasmaticas y eritrocitarias mediante electroforesis.Se describen los fenotipos de haptoglobina (Hp), esterasa D (ESD) y fosfoglucomutasa (PGM1) y las cantidades absolutas y porcentuales de proteinas totales, albuminas, alfa 1, alfa 2, beta y gamma globulinas presentes en el plasma.Se encontro una variante de ESD. Las mujeres tienen valores superiores de gamma globulina plasmatica
Subject(s)
Child , Humans , Male , Female , Blood Proteins , ErythrocytesSubject(s)
Blood Proteins/metabolism , Hematocrit , Hemoglobins/metabolism , Iron/blood , Age Factors , Child , Chile , Female , Humans , Male , Sex Factors , Transferrin/metabolismABSTRACT
Se describen los promedios, desviaciones tipicas, correlaciones y distribuciones de proteinemia, ferremias, capacidad total de fijacion de hierro, capacidad latente de fijacion de hierro, hematocrito, cantidad de hemoglobina y concentracion de hemoglobina corpuscular media, encontrados en una amuestra de aproximadamente 200 varones y 200 mujeres escolares del Area Norte de Santiago. Se describen ademas la relacción de estas variables con la edad que fluctuo entre 9 y 13 años
Subject(s)
Iron , Blood Proteins , Hematocrit , HemoglobinsABSTRACT
1. Se presenta la respuesta a la administracion oral y parenteral de Vitamina E,evaluada por la modificacion del test de Hemolisis por peroxido de hidrogeno en recien nacidos de bajo peso de nacimiento entre los 32 y 83 dias de vida. 2. Con la administracion de Vitamina E oral no se observo normalizacion del test en un periodo de siete dias despues de administrada. Con la Vitamina E parenteral,en cambio, los valores se normalizaron en el mismo plazo