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1.
Sud Med Ekspert ; 37(4): 12-4, 1994.
Article in Russian | MEDLINE | ID: mdl-7892683

ABSTRACT

Lipid content was measured in lung tissue of live and stillborn newborns with and without putrefactive changes by biochemical method of lipid extraction by hexane from dried fragments. Biochemical detection of high levels of lipids in pulmonary tissue from newborns both with and without putrefactive changes was found to be an evidence of intrauterine asphyxia caused by aspiration of amniotic fluid, and, hence, this method helped assess the contribution of intrauterine asphyxia to the origin and cause of death. The results are of paramount importance for forensic medicine, particularly so in cases when putrefied corpses of newborns have to be examined and there is no data on the course of pregnancy and labor.


Subject(s)
Fetal Hypoxia/diagnosis , Lipids/analysis , Lung/chemistry , Postmortem Changes , Autopsy , Fetal Death/diagnosis , Fetal Death/metabolism , Fetal Death/pathology , Fetal Hypoxia/metabolism , Fetal Hypoxia/pathology , Humans , Infant, Newborn , Lung/pathology
2.
Sud Med Ekspert ; 36(4): 5-7, 1993.
Article in Russian | MEDLINE | ID: mdl-8296351

ABSTRACT

Biochemical method of lipid extraction with hexane permits the diagnosis of functional exhaustion of adrenals resultant from prolonged stress using both fresh material and that changed by putrefaction when histologic and histochemical methods of investigation are useless. These data may be used in examinations of putrescent corpses to assess thanatogenesis and establish the cause of death.


Subject(s)
Adrenal Glands/chemistry , Lipids/analysis , Postmortem Changes , Stress, Physiological/pathology , Adolescent , Adrenal Glands/pathology , Adult , Aged , Body Water/chemistry , Cause of Death , Female , Humans , Male , Middle Aged , Stress, Physiological/metabolism , Stress, Physiological/mortality , Time Factors
3.
Sud Med Ekspert ; 36(2): 6-8, 1993.
Article in Russian | MEDLINE | ID: mdl-7940639

ABSTRACT

The biochemical method of lipid level assessment by their hexane extraction permits a judgement on the presence of obesity (fatty dystrophy) in the myocardium in drastic putrefactive changes in the corpse, when other methods are useless. Use of this method in cases with putrefactive changes will help in many cases to decide the problem on the possible 'cardiac' mechanism of death.


Subject(s)
Lipid Metabolism , Myocardium/metabolism , Myocardium/pathology , Postmortem Changes , Adult , Body Water/metabolism , Female , Humans , Male , Middle Aged , Time Factors
4.
Sud Med Ekspert ; 35(4): 26-9, 1992.
Article in Russian | MEDLINE | ID: mdl-1481236

ABSTRACT

Alcohol abuse involves heart mass increment at the expense of epicardial fat. Epicardial fat mass and the content of intramuscular lipids are the highest in alcoholic cardiomyopathy. Since epicardial fat mass increase is in direct correlation with the elevation of lipid content in the heart muscle proper, detection of a large mass of epicardial fat at autopsy permits a conclusion on myocardial obesity.


Subject(s)
Alcoholic Intoxication/pathology , Alcoholism/pathology , Cardiomyopathy, Alcoholic/pathology , Lipids/analysis , Myocardium/pathology , Alcoholic Intoxication/metabolism , Alcoholism/metabolism , Autopsy , Cardiomyopathy, Alcoholic/metabolism , Humans , Middle Aged , Myocardium/chemistry , Organ Size
5.
Sud Med Ekspert ; 34(2): 8-11, 1991.
Article in Russian | MEDLINE | ID: mdl-1882415

ABSTRACT

In the course of investigation it was stated that fat hepatic dystrophy can be diagnosed by biochemical method (lipid extraction by hexane). For al this the content of extracted lipids more than 20% of dry residue evidences about the presence of fat hepatic dystrophy which is confirmed by histological detection of lipid granules whose quantity is increasing from fat dystrophy I degree (lipid content amounts up to 30% of dry residue) to fat dystrophy III degree (lipid content is more than 40%). Formalin treatment doesn't produce significant changes in lipid content and doesn't prevent biochemical diagnosis of fat dystrophy of the liver. In case of putrefied liver lipid content is increased slightly but in all periods of putrefaction lipid content in the liver in case of fat dystrophy remains significantly higher than in controls. Biochemical method may be used in diagnosis of fat hepatic dystrophy and its degree not only on fresh and formalin-treated cadaveric material but also in case of markedly pronounced putrefactive changes when other methods are useless and this fact is of great value for medicolegal practice.


Subject(s)
Fatty Liver/diagnosis , Formaldehyde/pharmacology , Liver/pathology , Postmortem Changes , Body Water/metabolism , Cadaver , Fatty Liver/metabolism , Fatty Liver/pathology , Histocytochemistry , Humans , Lipid Metabolism , Liver/drug effects , Liver/metabolism , Muscles/metabolism , Muscles/pathology , Tissue Preservation
7.
Sud Med Ekspert ; 32(4): 10-2, 1989.
Article in Russian | MEDLINE | ID: mdl-2617546

ABSTRACT

The results of biochemical analysis of rabbit lungs in case of death due to fat embolism and mechanical asphyxia (control) are presented. Reliable difference in lipid quantities was evident both immediately after death and in different putrefaction periods (this difference was 9-10 times greater in case of pulmonary fat embolism than in controls). The significant reduction in water content of the lungs in case of fat embolism as compared to controls was detected. Histological analysis of the putrefactive lungs can't detect fat embolism. Biochemical analysis makes it possible to diagnose fat embolism of the lungs in case of their marked putrefactive changes.


Subject(s)
Embolism, Fat/diagnosis , Lung Diseases/diagnosis , Postmortem Changes , Animals , Embolism, Fat/metabolism , Embolism, Fat/pathology , Extravascular Lung Water/analysis , Lipids/analysis , Lung/analysis , Lung/pathology , Lung Diseases/metabolism , Lung Diseases/pathology , Rabbits , Time Factors
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