Associations of Serum Irisin and Fibroblast Growth Factor-21 Levels With Bone Mineral Characteristics in Eumenorrheic Adolescent Athletes With Different Training Activity Patterns.

PURPOSE: To describe serum irisin and fibroblast growth factor-21 (FGF-21) concentrations in healthy female adolescents with different training activity patterns and their associations with bone mineral properties and metabolic markers. METHODS: A total of 62 adolescent girls aged 14-18 years were recruited: 22 rhythmic gymnasts, 20 swimmers, and 20 untrained controls. Bone mineral characteristics by dual-energy X-ray absorptiometry, daily energy intake by dietary recall, serum irisin, FGF-21, undercarboxylated osteocalcin, and C-terminal telopeptide of type I collagen were measured in all girls. RESULTS: Whole body and lumbar spine areal bone mineral density and lumbar spine bone mineral content were higher in the rhythmic gymnasts group compared with swimmers and untrained controls groups (P < .05). Serum irisin, FGF-21, undercarboxylated osteocalcin, and C-terminal telopeptide of type I collagen levels were not significantly different between the groups. In the rhythmic gymnasts group, serum FGF-21 concentration was positively correlated with lumbar spine areal bone mineral density independently of confounding factors (r = .51; P = .027). CONCLUSIONS: Serum irisin and FGF-21 levels were not different between adolescent eumenorrheic girls with different training activity patterns. FGF-21 was positively associated with lumbar spine areal bone mineral density, which predominantly consists of trabecular bone in adolescent rhythmic gymnasts.

Follistatin Is Associated with Bone Mineral Density in Lean Adolescent Girls with Increased Physical Activity.

Follistatin is a member of the activin-follistatin-inhibin hormonal system and is proposed to affect bone metabolism. However, data regarding the effect of follistatin on bone are relatively scarce and contradictory in humans. The purpose of the current study was to investigate possible associations of serum follistatin concentration with bone mineral characteristics in lean and physically active adolescent girls. Bone mineral density, body composition, resting energy expenditure and different energy homeostasis hormones in serum including follistatin, leptin and insulin were investigated. Significant relationships (p < 0.05) between serum follistatin (1275.1 ± 263.1 pg/mL) and whole-body (WB) bone mineral content (r = 0.33), WB areal bone mineral density (aBMD) (r = 0.23) and lumbar spine (LS) aBMD (r = 0.29) values were observed. Serum follistatin remained associated with LS aBMD independent of body fat and lean masses (r = 0.21; p < 0.05). However, the follistatin concentration explained only 3% (R2 × 100; p = 0.049) of the total variance in LS aBMD values. In conclusion, serum follistatin concentrations were associated with bone mineral values in lean adolescent girls with increased physical activity. Follistatin was an independent predictor of lumbar spine areal bone mineral density, which predominantly consists of trabecular bone.

Long-Term Rowing Performance Development in Male Olympic and World Championship Medal Winners Compared With Nonmedalists.

ABSTRACT: Mäestu, J, Lelle, R, Mäestu, E, Pind, R, Vahtra, E, Purge, P, and Mikulic, P. Long-term rowing performance development in male Olympic and World Championship medal winners compared with nonmedalists. J Strength Cond Res 37(9): e521-e526, 2023-The purpose of this study was, first, to investigate individual longitudinal 2,000 m rowing ergometer performance (2,000 erg) development of world class male rowers from the beginning of their career until reaching the elite level and to compare ELITE performance development with those who did not reach podium places and, second, to provide 2,000 erg milestones for talent prediction in rowing. Individual annual 2,000 erg performances of 54 male rowers were analyzed from age 15 and throughout their career. Olympic or World Championships medal winners (ELITE; n = 11) were compared with those who did not reach international podium places. Two thousand erg increased ( p ≤ 0.05) until the age of 24 in ELITE, while plateaued earlier for less successful rowers. No differences in the rate of performance improvement were found between ELITE and lower performers ( p > 0.05). At the age of 20, performance between ELITE and international level nonmedal winners reached significant difference (480.7 ± 20.9 W vs. 435.3 ± 33.9 W, respectively) and remained different onwards ( p ≤ 0.05). However, the average of the 10 best seasons cross-sectional performances of less successful rowers were significantly higher until age 18, compared with ELITE. ELITE rowers were able to improve their 2,000 erg performance to higher age; therefore, it is difficult to predict elite performers in junior rowers. Cross-sectional performances of less successful rowers may indicate temporary high-level performers, who might not be able to advance the elite level.

Changes in irisin, inflammatory cytokines and aerobic capacity in response to three weeks of supervised sprint interval training in older men.

BACKGROUND: This study investigated the effects of supervised short-term sprint interval training (SIT) on circulating irisin, interleukin (IL)-6 and tumour necrosis factor (TNF)-α concentrations, and aerobic capacity and body composition values in healthy older men. METHODS: Eleven older men (63±8 years; 178.0±5.5 cm; 82.7±8.6 kg; 22.7±3.7% body fat) underwent SIT (6 repetitions of 30 s all-out cycling bouts with 4 min active recovery after each bout) three days a week for three consecutive weeks. Body composition measured by dual-energy X-ray absorptiometry, aerobic capacity assessed by direct peak oxygen consumption (VO2peak) test and morning fasting blood samples were obtained before and after a 3-week SIT intervention. RESULTS: Nine supervised SIT sessions moderately (effect size [ES] =0.65; P<0.05) increased irisin concentrations (from 135.40±28.73 to 154.20±47.09 ng.mL-1) together with moderate decreases (P<0.05) in IL-6 (ES=0.89; from 1.26±0.44 to 0.87±0.44 pg.mL-1) and TNF-α (ES=0.64; from 5.10±1.23 to 4.31±1.20 pg.mL-1) levels in older men. In addition, increase in VO2peak was significant but small (ES=0.25; P<0.05; from 36.0±7.1 to 37.8±6.7 mL.min.-1kg-1), while no changes (P>0.05) in body composition variables were observed after a short-term SIT period. CONCLUSIONS: A 3-week SIT intervention with only nine training sessions increased circulating irisin concentrations, improved inflammatory profile and aerobic capacity without changes in body composition in healthy older men. Accordingly, a short-term SIT programme is a time efficient alternative for traditional aerobic training to improve metabolic health and aerobic capacity in older adults.

Session Rating of Perceived Exertion Is Different for Similar Intensity and Duration Prescribed Low-Intensity Sessions and Has a Different Effect on Performance in Young Cross-Country Skiers.

ABSTRACT: Pind, R, Purge, P, Mäestu, E, Vahtra, E, Hofmann, P, and Mäestu, J. Session rating of perceived exertion is different for similar intensity and duration prescribed low-intensity sessions and has a different effect on performance in young cross-country skiers. J Strength Cond Res 37(1): 187-193, 2023-The aim of this study was to analyze whether the rating of perceived exertion (RPE) and the resulting internal load (sRPE) could differ among subjects when volume and intensity are matched in young cross-country (XC) skiers. Second, we analyzed whether subjects with a high or low RPE response and sRPE have different adaptation after a 1-week low-intensity high-volume training period. Well-trained national level XC skiers (n = 13) aged 13.4 ± 1.9 years took part in a 1-week training period with double poling performance tested pretraining and after 1-week training. All subjects trained under a similar training program. Rating of perceived exertion was measured after each training session on a 10-point scale, and the total training load was calculated by the sRPE method by multiplying training session's duration and the RPE. Subjects were divided into Glow (n = 6) or Ghigh (n = 7) groups based on median rating of RPE (3.78 ± 1.24) in low-intensity trainings. Total training load in low-intensity trainings was higher in Ghigh compared with Glow (4,010 ± 765 vs. 2,499 ± 193 arbitrary units, respectively), although total training time with (16.3 ± 0.8 hours vs. 16.0 ± 0.6 hours) or without (13.1 ± 0.9 hours vs. 13.3 ± 0.7 hours, p > 0.05, respectively to Glow and Ghigh) high-intensity sessions was not different. No significant differences were found for time spent in different heart rate zones between the 2 groups. Increases in performance were significant for both groups (p< 0.05), while the effect was large in Ghigh (+12.5%; ES = 0.74), and medium in Glow (+10.7%; ES = 0.34). Differences in the internal load and the further quantification of training load for different target intensity sessions could provide important practical information to stimulate expected adaptation in young endurance athletes.

Pubertal increment in insulin resistance is negatively related to lumbar bone mineral density in 18-year-old males.

Transient insulin resistance seen during puberty is expected to favour body growth, but our results show that increment in insulin resistance even in physiological ranges during puberty might compromise lumbar spine bone mineral density accrual independently of body composition parameters, and therefore adult bone quality might be challenged. INTRODUCTION: Insulin resistance (IR) might have a compromising effect on growing bone, and therefore adult bone quality might be challenged. The aim of the present study was to identify whether increases in IR during puberty contribute to bone mineral characteristics in males independently of body composition parameters. METHODS: This is a retrospective cohort-based longitudinal observational study. Data from 85 subjects were included. Boys were studied annually during their pubertal years (12 years at baseline) and at follow-up at the age of 18 years. Anthropometry, bone age, fasting blood samples, body composition, total body, and lumbar spine bone mineral characteristics were measured. Insulin resistance was determined by homeostatic model assessment of IR (HOMA-IR). Multiple regression analysis was performed to determine the effect of changes in HOMA-IR during pubertal years as a longitudinal predictor to fixed bone mineral outcome variables at the age of 18 years. All models were adjusted to potential clinically justified confounding variables. RESULTS: After adjustment to baseline bone indices and body composition-related predictors, the pubertal increment in the HOMA-IR was a negative independent predictor of lumbar spine bone mineral areal density (ß = - 0.202, p = 0.005) and lumbar spine bone mineral apparent density (ß = - 0.235, p = 0.005) in 18-year-old males. CONCLUSIONS: Pubertal increment in IR has a potential diminishing effect on lumbar spine bone mineral density accrual independently of body composition parameters. Further studies are needed to clarify whether monitoring HOMA-IR during puberty may identify subjects at increased risk of low peak bone mass and possible osteoporosis in the future.

Associations of Circulating Irisin and Fibroblast Growth Factor-21 Levels with Measures of Energy Homeostasis in Highly Trained Adolescent Rhythmic Gymnasts.

The aim of this investigation was to determine the associations of serum irisin and fibroblast growth factor-21 (FGF-21) with the measures of energy homeostasis, training stress and other energy homeostasis hormones in highly trained adolescent rhythmic gymnasts (RG). Thirty-three RG and 20 untrained controls (UC) aged 14−18 years participated in this study. Body composition, resting energy expenditure (REE), peak oxygen consumption, and different energy homeostasis hormones in serum, including irisin, FGF-21, leptin, and resistin, were measured. Irisin and FGF-21 were not significantly different (p > 0.05) between RG and UC groups. In RG, serum irisin was positively associated with REE (r = 0.40; p = 0.021) and leptin (r = 0.60; p = 0.013), while serum FGF-21 was related to body fat mass (r = 0.46; p = 0.007) and leptin (r = 0.45; p = 0.009). Irisin was related to FGF-21, independent of age, body fat, and lean masses (r = 0.36; p = 0.049) in RG. In conclusion, serum irisin concentration was associated with energy expenditure and serum FGF-21 level with energy availability measures in lean adolescent athletes, while no relationships of irisin and FGF-21 with energy status measures were observed in lean nonathletic adolescents.

Effect of Short-Duration High-Intensity Upper-Body Pre-Load Component on Performance among High-Level Cyclists.

The aim of the present study was to evaluate the effects of upper-body high-intensity exercise priming on subsequent leg exercise performance. Specifically, to compare maximal 4000 m cycling performance with upper-body pre-load (MPThigh) and common warm-up (MPTlow). In this case, 15 high-level cyclists (23.3 ± 3.6 years; 181 ± 7 cm; 76.2 ± 10.0 kg; V˙O2max: 65.4 ± 6.7 mL·kg−1·min−1) participated in the study attending three laboratory sessions, completing an incremental test and both experimental protocols. In MPThigh, warm-up was added by a 25 s high-intensity all-out arm crank effort to the traditional 20-min aerobic warm-up. Both 4000 m maximal bouts started with a 12 s all-out start. Heart rate, blood lactate concentration [La) and spirometric data were measured and analyzed. Overall MPThigh time was slower by 5.3 ± 1.2 s (p < 0.05). [La] at the start was 5.5 ± 1.5 mmol·L−1 higher for MPThigh (p < 0.001) reducing anaerobic energy contribution which was higher in MPTlow during the first and third 1000 m split (p < 0.05). Similarly, MPTlow maintained higher total average power during the entire performance (p < 0.05, d = 0.7). Although the MPThigh condition performed less effectively due to decreased anaerobic capacity, pre-load effect may have the potential to enhance performance at longer distances.

The Effect of Different Periodization and Modes of Concurrent Strength and Endurance Training on Double Poling Performance and Body Composition in Adolescent Cross-Country Skiers.

The aim of the study was to compare the effects of different types and periodization of strength training on body composition and maximal aerobic performance in 10-week training period in adolescent XC skiers. Twenty-eight adolescent competitive cross-country skiers, including 10 females (age 17.9 ± 1.8 years; body mass 69.6 ± 9.7 kg; height 1.77 ± 0.1 m; training experience 8.6 ± 3.2 years) took part in this study. Pre-and post-intervention performance was measured with the incremental exercise test (Pmax) on a double poling ski ergometer. Changes in body composition were measured with DXA. In addition to regular endurance training, experimental group one (EXP1) performed maximal and explosive strength training two times per week, experimental group two (EXP2) performed maximal and explosive strength training 1-3 times per week, and the traditional (TRAD) group performed low intensity-high volume strength training 2 times per week. Increases in arm, trunk, and overall lean mass were found in TRAD (p < 0.05). Increases in arm lean-mass was found in EXP1 (p < 0.05), while no changes in body composition occurred in EXP2 (p ≥ 0.05). Pmax improved significantly in all groups (p < 0.05). Changes in body mass, overall and arm lean mass was related to changes in absolute performance (W; p < 0.05), while no relationships were found between changes in body composition parameters and relative performance (W/kg; p ≥ 0.05). In conclusion, different periodization of strength training led to similar improvements in double poling ergometer performance, but resulted in different changes in body composition (lean mass) in adolescent cross-country skiers.

Corrigendum: Increases in RPE Rating Predict Fatigue Accumulation Without Changes in Heart Rate Zone Distribution After 4-Week Low-Intensity High-Volume Training Period in High-Level Rowers.

[This corrects the article DOI: 10.3389/fphys.2021.735565.].

Leptin to adiponectin ratio in puberty is associated with bone mineral density in 18-year-old males.

INTRODUCTION: Inconsistent associations of leptin and adiponectin with bone mineral characteristics in puberty and adolescence have been reported. We aimed to examine the associations between leptin to adiponectin ratio (LAR) in puberty and bone mineral characteristics at the age of 18 years in healthy males. MATERIALS AND METHODS: 88 white Caucasian boys were investigated at T1 (mean age 12.1 years), T2 (14.0 years) and T3 (18.0 years). Serum leptin and adiponectin were measured and LAR was calculated at T1, T2 and T3, bone mineral density (BMD) and bone mineral apparent density (BMAD) for total body and lumbar spine (LS) at T1 and T3. Spearman correlation coefficient and partial correlation analyses were used to describe the associations between mean pubertal LAR and BMD at T3. RESULTS: Mean pubertal LAR was negatively correlated with both LS BMD (r = -0.23; P < 0.05) and LS BMAD at T3 (r = -0.33; P < 0.05). These associations remained significant also in partial correlation analysis after controlling for total body fat percentage, total testosterone, HOMA-IR and physical activity at T1 (r = -0.31; P < 0.05 and r = -0.41; P < 0.05 respectively). CONCLUSION: LAR in puberty is negatively associated with lumbar spine BMD and lumbar spine BMAD at the age of 18 years.

Can We Improve the Functional Threshold Power Test by Adding High-Intensity Priming Arm-Crank?

The aim of the present study was to evaluate the effects of arm-crank induced priming on subsequent 20 min Functional Threshold Power Test among 11 well-trained male cyclists (18.8 ± 0.9 years; 182 ± 5 cm; 73.0 ± 6.6 kg; V˙O2max 67.9 ± 5.1 mL·kg-1·min-1). Participants completed an incremental test and two maximal performance tests (MPTs) in a randomized order. Warm-up prior to MPTlow consisted of 20 min aerobic exercise and 25 s high-intensity all-out arm crank effort was added to warm-up in MPThigh. Constant intensities for the first 17 min of MPT were targeting to achieve a similar relative fatigue according to participants' physiological capacity before the last 3 min all-out spurt. Final 3 min all-out spurt power was 4.94 ± 0.27 W·kg-1 and 4.85 ± 0.39 W·kg-1 in MPTlow and MPThigh, respectively (not statistically different: p = 0.116; d = 0.5). Blood lactate [La] levels just before the start were higher (p < 0.001; d = 2.6) in MPThigh (5.6 ± 0.5 mmol·L-1) compared to MPTlow (1.1 ± 0.1 mmol·L-1). According to V˙CO2 and net [La] data, significantly higher anaerobic energy production was detected among MPTlow condition. In conclusion, priming significantly reduced anaerobic energy contribution but did neither improve nor decrease group mean performance although effects were variable. We suggest priming to have beneficial effects based on previous studies; however, the effects are individual and additional studies are needed to distinguish such detailed effects in single athletes.

Increases in RPE Rating Predict Fatigue Accumulation Without Changes in Heart Rate Zone Distribution After 4-Week Low-Intensity High-Volume Training Period in High-Level Rowers.

Purpose: The aim of this study was to investigate the interaction of training load quantification using heart rate (HR) and rating of perceived exertion (RPE)-based methodology, and the relationship between internal training load parameters and subjective training status (Fatigue) in high-level rowers during volume increased low-intensity training period. Methods: Training data from 19 high-level rowers (age 23.5 ± 5.9 years; maximal oxygen uptake 58.9 ± 5.8 ml·min-1·kg-1) were collected during a 4-week volume increased training period. All individual training sessions were analyzed to quantify training intensity distribution based on the HR time-in-zone method (i.e., HR Z1, HR Z2, and HR Z3) determined by the first and second ventilatory thresholds (VT1/VT2). Internal training load was calculated using session RPE (sRPE) to categorize training load by effort (i.e., sRPE1, sRPE2, and sRPE3). The Recovery-Stress Questionnaire for Athletes (RESTQ-Sport) questionnaire was implemented after every week of the study period. Results: No differences were found between the respective HR and effort-based zone distributions during the baseline week (p > 0.05). Compared to HR Z1, sRPE1 was significantly lower in weeks 2-4 (p < 0.05), while sRPE2 was higher in weeks 2-3 compared to HR Z2 (p < 0.05) and, in week 4, the tendency (p = 0.06) of the higher amount of sRPE3 compared to HR Z3 was found. There were significant increases in RESTQ-Sport stress scales and decreases in recovery scales mostly during weeks 3 and 4. Increases in the Fatigue scale were associated with the amounts of sRPE2 and sRPE3 (p = 0.011 and p = 0.008, respectively), while no associations with Fatigue were found for HR-based session quantification with internal or external training load variables. Conclusion: During a low-intensity 4-week training period with increasing volume, RPE-based training quantification indicated a shift toward the harder rating of sessions with unchanged HR zone distributions. Moderate and Hard rated sessions were related to increases in Fatigue. Session rating of perceived exertion and effort-based training load could be practical measures in combination with HR to monitor adaptation during increased volume, low-intensity training period in endurance athletes.

The Associations of Body Image Perception with Serum Resistin Levels in Highly Trained Adolescent Estonian Rhythmic Gymnasts.

Rhythmic gymnasts (RGs) are more likely to be dissatisfied with their body mass and shape compared to untrained controls (UCs). However, due to the lack of information, the aim of this study was to investigate the associations of body image perception (BIP) with body composition, daily energy consumption and different blood biochemical markers in adolescent RGs compared to UCs. Thirty-three highly trained RG girls and 20 UC girls aged 14-18 years participated in this cross-sectional study. Height, body mass, body composition, energy intake, resting energy expenditure, training volume and different blood biochemical markers were measured. The body attitude test (BAT) was used to evaluate the BIP of the participants. There were no differences in the total BAT scores between the groups. In RGs, the BAT score correlated positively with the serum resistin level (r = 0.35; p = 0.047). A stepwise multiple regression analysis showed that 40.8% of the variability in the BAT score was determined by resistin and BMI. The association of BIP with resistin values was observed only in RGs. In conclusion, our findings add to the increasing evidence that resistin may be a link between BIP and body composition, most likely through fat mass, in adolescent female RGs.

Irisin, Fibroplast Growth Factor-21, and Follistatin Responses to Endurance Rowing Training Session in Female Rowers.

Purpose: This study examined selected myokine responses to an endurance rowing training session, and whether metabolic demands of the acute aerobic rowing exercise together with training volume, aerobic capacity, and body composition variables affect potential exercise-induced changes in the myokine levels in female rowers. Methods: Fifteen national level female rowers [18.3 ± 1.6 years; 172.0 ± 5.0 cm, 67.5 ± 8.8 kg; maximal oxygen consumption (VO2max): 47.2 ± 7.9 ml.min.-1 kg-1] performed a 1-h rowing ergometer exercise at the intensity of 70% of VO2max [distance: 12.1 ± 1.1 km; energy expenditure (EE): 639 ± 69 kcal; heart rate (HR): 151 ± 7 beats.min-1] followed by a 30-min recovery period. Venous blood samples were collected before and after exercise, and analyzed for irisin, fibroplast growth factor-21 (FGF-21), and follistatin conentrations. Results: Plasma irisin and FGF-21 concentrations were increased (by 8%; p = 0.013 and by 13%; p < 0.0001, respectively) immediately after the aerobic rowing exercise. Follistatin was significantly increased (by 11%; p = 0.001) only after the first 30 min of recovery. Exercise metabolic demand variables such as distance covered and total EE were correlated with the pre-to-post-exercise increases in FGF-21 concentrations (r = 0.52; p = 0.047 and r = 0.68; p = 0.005, respectively). Exercise-induced increases in irisin levels were related to aerobic capacity as measured by VO2max (r = 0.53; p = 0.041) and training stress as measured by weekly training volume (r = 0.54; p = 0.039) in female rowers. Conclusion: Acute negative energy balance induced by a single endurance rowing training session elicited significant increases in irisin, FGF-21, and follistatin levels in national level female rowers. While exercise-induced increases in FGF-21 levels were associated with exercise metabolic demand measures, exercise-induced increases in irisin concentrations were related to aerobic capacity and training stress measures in female rowers.

Pubertal Physical Activity and Cardiorespiratory Fitness in Relation to Late Adolescent Body Fatness in Boys: A 6-Year Follow-Up Study.

There is a lack of studies investigating whether objectively measured physical activity (PA) and cardiorespiratory fitness (CRF) in puberty is associated with healthier body composition in late adolescence. The study sample included 71 boys, who were measured at puberty (mean age of 12.1 years) and again at late adolescence (mean age of 18.1 years). Physical activity was measured by accelerometry, and total PA, moderate-to-vigorous PA (MVPA), and sedentary time (SED) were calculated, while CRF was assessed by a peak oxygen consumption test. Body composition was measured by dual-energy X-ray absorptiometry, and body fat percentage (%BF), fat mass index (FMI), trunk fat mass index (TFMI), and fat-free mass index (FFMI) were calculated. Body mass index (BMI) and SED time increased, while MVPA, total PA, and CRF decreased from puberty to late adolescence. A relatively high degree of tracking for BMI and CRF, and a low tracking for PA from puberty to late adolescence was observed. Only the CRF value at puberty negatively predicted adolescent BF% (ß = -0.221; p = 0.015), FMI (ß = -0.212; p = 0.006), and TFMI (ß = -0.189; p = 0.015) values. Adolescents whose CRF was above the median at puberty had lower BMI, BF%, FMI, and TFMI in comparison to those whose CRF was below the median at puberty. In conclusion, measured CRF and PA values decreased from puberty to late adolescence. Pubertal CRF predicted body fatness indices six years later in healthy adolescents.

The Effect of Lower Body Anaerobic Pre-Loading on Upper Body Ergometer Time Trial Performance.

Pre-competitive conditioning has become a substantial part of successful performance. In addition to temperature changes, a metabolic conditioning can have a significant effect on the outcome, although the right dosage of such a method remains unclear. The main goal of the investigation was to measure how a lower body high-intensity anaerobic cycling pre-load exercise (HIE) of 25 s affects cardiorespiratory and metabolic responses in subsequent upper body performance. Thirteen well-trained college-level male cross-country skiers (18.1 ± 2.9 years; 70.8 ± 7.6 kg; 180.6 ± 4.7 cm; 15.5 ± 3.5% body fat) participated in the study. The athletes performed a 1000-m maximal double-poling upper body ergometer time trial performance test (TT) twice. One TT was preceded by a conventional low intensity warm-up (TTlow) while additional HIE cycling was performed 9 min before the other TT (TThigh). Maximal double-poling performance after the TTlow (225.1 ± 17.6 s) was similar (p > 0.05) to the TThigh (226.1 ± 15.7 s). Net blood lactate (La) increase (delta from end of TT minus start) from the start to the end of the TTlow was 10.5 ± 2.2 mmol L-1 and 6.5 ± 3.4 mmol L-1 in TThigh (p < 0.05). La net changes during recovery were similar for both protocols, remaining 13.5% higher in TThigh group even 6 min after the maximal test. VCO2 was lower (p < 0.05) during the last 400-m split in TThigh, however during the other splits no differences were found (p < 0.05). Respiratory exchange ratio (RER) was significantly lower in TThigh in the third, fourth and the fifth 200 m split. Participants individual pacing strategies showed high relation (p < 0.05) between slower start and faster performance. In conclusion, anaerobic metabolic pre-conditioning leg exercise significantly reduced net-La increase, but all-out upper body performance was similar in both conditions. The pre-conditioning method may have some potential but needs to be combined with a pacing strategy different from the usual warm-up procedure.

Serum sclerostin concentration is associated with specific adipose, muscle and bone tissue markers in lean adolescent females with increased physical activity.

OBJECTIVES: Sclerostin is an important regulator of bone mass involving the Wnt/ß-catenin signalling pathway. Relatively few studies have investigated the relationships of circulating sclerostin levels with adiposity-related and muscle-related biochemical factors in individuals with increased energy metabolism. The aim of this study was to investigate the associations of circulating sclerostin with adipokines, myokines, osteokines and body composition values in lean adolescent females with increased physical activity. METHODS: A total of 73 adolescent females who were physically active and aged 14-18 years old participated in the study. Sclerostin, leptin, resistin, tumour necrosis factor (TNF)-α, interleukin (IL)-6, irisin, osteocalcin, C-terminal telopeptide of type I collagen (CTx), insulin-like growth factor (IGF)-1 and insulin were obtained from fasting blood samples. Body composition was measured by dual-energy X-ray absorptiometry (DXA) and analyzed for body fat mass, lean body mass, bone mineral content and muscle mass. RESULTS: Serum sclerostin (117.9 ± 60.3 pg/mL) was correlated with age, age at menarche, body fat, muscle mass, training activity, leptin, TNF-α, irisin, osteocalcin, CTx and IGF-1. Multivariate linear regression analysis demonstrated that fat mass (ß = 0.434; p = 0.001), leptin (ß = -0.308; p = 0.015), irisin (ß = 0.227; p = 0.024) and CTx (ß = 0.290; p = 0.031) were the most important predictors of serum sclerostin concentration. CONCLUSIONS: Bone-derived sclerostin is associated with specific adipokine, myokine and osteokine values in lean adolescent females with increased physical activity. These results suggest that the interactions between bone, adipose and muscle tissues could also be associated with circulating sclerostin concentrations.

Serum sclerostin and cytokine responses to prolonged sculling exercise in highly-trained male rowers.

This study examined sclerostin and cytokine responses to an endurance training session in male single sculling rowers. Sixteen highly trained rowers performed a 2-h rowing exercise (distance: 23.8 ± 0.9 km; heart rate: 138 ± 8  beats.min-1; intensity: 79.8 ± 2.1% of the anaerobic threshold). Rowing resulted significant increment (p < 0.05) in sclerostin (36%), interleukin (IL)-6 (345%), IL-10 (129%) and monocyte chemoattractant protein-1 (MCP-1) (132%), while increases in irisin (6%) and tumour necrosis factor-α (3%) were not significant (p > 0.05). There was a correlation between the pre-to-post-exercise increase in sclerostin and the distance covered (r = 0.67; p = 0.005) as a marker of energy expenditure, and weekly training volume (r = 0.66; p = 0.005) as a marker of training stress. Post-exercise gain in MCP-1 was the most important predictor of post-exercise gain in sclerostin (ß = 0.543; p = 0.040). In conclusion, acute non-impact rowing training session with a total body mode induced increases in sclerostin and also in IL-6, IL-10 and MCP-1 concentrations, while post-exercise gain in MCP-1 was the main determinant of post-exercise gain in sclerostin. Exercise-induced increase in sclerostin could be regarded as a signal for metabolic reaction to the energy cost of acute exercise in rowers.

Internal Load From Hard Training Sessions Is Related to Changes in Performance After a 10-Week Training Period in Adolescent Swimmers.

ABSTRACT: Pind, R, Mäestu, E, Purge, P, Jürgenson, J, Arend, M, and Mäestu, J. Internal load from hard training sessions is related to changes in performance after a 10-week training period in adolescent swimmers. J Strength Cond Res 35(10): 2846-2852, 2021-The purpose of this study was to investigate the association between session rating of perceived exertion (sRPE) and its categorization with the changes in swimming performance in adolescent swimmers. National level swimmers (age 16.4 ± 2.1 years, V̇o2max 50.0 ± 4.0 ml·min-1·kg-1) participated in a 10-week period preceding the National Winter Championships. Pre-training and post-training physiological parameters and swimming performance were measured. All training bouts with sRPE data were recorded. In addition, trainings were categorized as light, moderate, or hard. For calculating the corresponding internal training load (ITL), 2 fixed categorizations were applied based on earlier published sources and an individualized categorization based on ventilatory thresholds. During the 10-week training period, large to very large increases were found in aerobic (effect size [ES] = 1.58) and anaerobic threshold power output W·kg-1 (ES = 2.46), respectively, and smaller, but significant, improvement in 100-m swimming performance (ES = 0.36). Total ITL during the 10-week period was significantly related to changes in anaerobic threshold (r = 0.81). Fixed and individualized sRPE methods from hard sessions were associated with changes in V̇o2max (r = 0.77) and performance of only 100-m leg kicks (r = 0.76), respectively. Internal training load was related to changes in performance variables during the 10-week study period. Using individualized sRPE values, the accumulation of the ITL from hard training sessions was associated with changes in V̇o2max and swimming performance. The aforementioned categorization could help coaches in appropriate application of ITL for assessing the total training load in adolescent swimmers.