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1.
Diabetes Obes Metab ; 17(12): 1150-7, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26264812

ABSTRACT

AIMS: To validate strategies to prevent exercise-induced hypoglycaemia via insulin-dose adjustment in adult patients with type 1 diabetes (T1D) on pump therapy. METHODS: A total of 20 patients randomly performed four 30-min late post-lunch (3 h after lunch) exercise sessions and a rest session: two moderate sessions [50% maximum oxygen consumption (VO2 max)] with 50 or 80% basal rate (BR) reduction during exercise + 2 h and two intense sessions (75% VO2 max) with 80% BR reduction or with their pump stopped. Two additional early post-lunch sessions (90 min after lunch) were analysed to compare hypoglycaemia incidence for BR reduction versus bolus reduction. RESULTS: In all, 100 late post-lunch sessions were analysed. Regardless of exercise type and BR reduction, no more hypoglycaemic events occurred in the period until the next morning than occurred after the rest sessions. In the afternoon, no more hypoglycaemic events occurred with 80% BR reduction/moderate exercise or with pump discontinuation/intense exercise than for the rest session, whereas more hypoglycaemic events occurred with 50% BR reduction/moderate exercise and 80% BR reduction/intense exercise. After early post-lunch exercise (n = 37), a trend towards fewer hypoglycaemic episodes was observed with bolus reduction versus BR reduction (p = 0.07). Mean blood glucose fell by ∼3.3 mmol/l after 30 min of exercise, irrespective of dose reduction, remaining stable until the next morning with no rebound hyperglycaemia. CONCLUSION: In adults with T1D, to limit the hypoglycaemic risk associated with 30 min of exercise 3 h after lunch, without carbohydrate supplements, the best options seem to be to reduce BR by 80% or to stop the pump for moderate or intense exercise, or for moderate exercise 90 min after lunch, to reduce the prandial bolus rather than the BR.


Subject(s)
Diabetes Mellitus, Type 1/drug therapy , Exercise , Hypoglycemia/prevention & control , Hypoglycemic Agents/administration & dosage , Insulin Infusion Systems , Insulin/administration & dosage , Adult , Algorithms , Blood Glucose/analysis , Cross-Over Studies , Diabetes Mellitus, Type 1/blood , Female , Humans , Hypoglycemia/chemically induced , Hypoglycemia/epidemiology , Hypoglycemic Agents/adverse effects , Hypoglycemic Agents/blood , Hypoglycemic Agents/therapeutic use , Incidence , Insulin/adverse effects , Insulin/blood , Insulin/therapeutic use , Lunch , Male , Middle Aged , Monitoring, Ambulatory , Oxygen Consumption/drug effects , Physical Exertion/drug effects , Postprandial Period , Risk , Single-Blind Method
2.
Eur J Clin Pharmacol ; 58(10): 649-52, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12610739

ABSTRACT

OBJECTIVE: To compare the intrarectal bioavailabilities of two parenteral formulations of quinine most available in French- (Cinchona alkaloid mixture) and English (hydrochloride salt) -speaking areas of Africa. METHODS: The pharmacokinetics of quinine was investigated in four groups of 12 children with acute Plasmodium falciparum malaria receiving 8 mg/kg quinine base every 8 h either as hydrochloride salt or Cinchona alkaloid mixture by a slow 4-h intravenous infusion or intrarectal administration. Body temperature and parasitaemia were monitored, and blood quinine concentrations were measured by means of high-performance liquid chromatography. RESULTS: At 72 h, all the children were aparasitaemic and apyretic. Quinine C(max) values were higher after intravenous infusion of the hydrochloride salt and Cinchona alkaloid mixture (6.9+/-1.9 micro g/ml and 5.2+/-1.3 micro g/ml) than after intrarectal administration (3.5+/-1.4 micro g/ml and 3.1+/-1.6 micro g/ml), but t(max) values were similar (3.6+/-1.5, 4.2+/-1.0, 4.0+/-1.9, and 4.7+/-2.0 h, respectively). Intrarectal relative bioavailabilities of hydrochloride salt solution (57%) and Cinchona alkaloid mixture (62%) were similar. CONCLUSION: Whatever the parenteral formulation of quinine, the blood concentration-time profiles of quinine were similar after intrarectal administration. Intrarectal administration of hydrochloride salt solution is a possible mode of quinine delivery in remote rural areas of Africa.


Subject(s)
Antimalarials/pharmacokinetics , Malaria, Falciparum/drug therapy , Quinine/pharmacokinetics , Acute Disease , Administration, Oral , Administration, Rectal , Africa , Antimalarials/administration & dosage , Antimalarials/blood , Area Under Curve , Biological Availability , Child , Child, Preschool , Humans , Infusions, Intravenous , Malaria, Falciparum/blood , Quinine/administration & dosage , Quinine/blood , Time Factors
3.
Sante ; 11(3): 145-53, 2001.
Article in French | MEDLINE | ID: mdl-11641075

ABSTRACT

Delay for treatment of severe malaria is the cause of an important childhood mortality in Africa especially in rural zone when health facilities and accessibility are scarce. Intrarectal treatment is of particular interest in children as a non aggressive, painless and easy treatment. It can be used as early treatment and could decrease the lethality of severe malaria. We recently showed the kinetic profile, the optimal regimen and the clinical efficacy of intrarectal quinine (QIR) using Quinimax (Sanofi, Gentilly France) 20 mg/kg in solution with 2 ml of water. From 1994 to 1996 two open clinical trials were performed in Niger in children (2-15 years). QIR was compared with intraveinous infusion in cerebral malaria (n = 76) and with intramuscular quinine in severe malaria (n = 57). A three daily QIR administration (20 mg/kg followed by 15 mg/kg/8 h) was used in cerebral malaria; a two daily administration in severe malaria (30 mg/kg followed by 20 mg/kg/12 h). Symptomatic treatment was associated for hyperthermia, hypoglycemia, anemia and seizures. Results. In the cerebral malaria study 58 children presented a Blantyre coma score below 3. Four children in the IR group and 9 children in the infusion group died (P > 0.05). Evolution was similar in both treatment groups: temperature clearance (< 37.5 degrees C) 39.0 +/- 15.2 h and 37.1 +/- 16.5 h; return to consciousness 34.6 +/- 12.8 h and 33.0 +/- 14.1 h; decrease to 50% of the initial parasites count: 15.5 +/- 11.5 h and 13.8 +/- 10.0 h. Residual blood quinine concentrations at 48 hours were similar 7.4 +/- 3.7 mg/l and 7.2 +/- 2.9 mg/l. In the severe malaria study, the mortality was 0 and 7.6% in the QIR and IM group respectively (P > 0.005). Evolution was similar in both treatment groups: temperature clearance (< 37.5 degrees C) 38.7 +/- 22.8 h and 38.6 +/- 22.2 h; return to consciousness 26.8 +/- 13.9 h and 27.6 +/- 9.9 h for the 16 children in coma. The evolution under QIR treatment was also similar with that described with the other quinine routes. QIR allows an efficious treatment particularly when correct infusion cannot be performed. The efficacy, the simplicity and the good tolerance of QIR are of major concern to decrease the mortality of severe malaria due to delay for treatment and to decrease the side-effects due to intramuscular administrations of quinine in Africa.


Subject(s)
Antimalarials/administration & dosage , Malaria, Cerebral/drug therapy , Quinine/administration & dosage , Administration, Rectal , Adolescent , Age Factors , Antimalarials/blood , Antimalarials/pharmacokinetics , Child , Child, Preschool , Drug Administration Schedule , Drug Monitoring , Glasgow Coma Scale , Humans , Infant , Infusions, Intravenous , Malaria, Cerebral/blood , Malaria, Cerebral/mortality , Malaria, Cerebral/parasitology , Niger/epidemiology , Quinine/blood , Quinine/pharmacokinetics , Severity of Illness Index , Survival Analysis , Time Factors , Treatment Outcome
4.
Liver ; 20(1): 88-92, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10726965

ABSTRACT

AIMS: In cirrhosis, the effects of selective activation of atrial natriuretic peptide (ANP) R2-receptors on haemodynamics, endogenous ANP (ANP1-28) and sodium excretion are unknown. The aim of the present study was to examine the effects of selective activation of ANP-R2 receptors by ANP4-23 (a ring-deleted analogue of endogenous ANP) on haemodynamics, plasma ANP1-28 concentrations and urinary sodium excretion in conscious cirrhotic and normal rats. METHODS: Haemodynamics and sodium excretion were measured prior to and following administration of ANP4 23. Plasma ANP1-28 concentrations were also measured. RESULTS: In cirrhotic rats, ANP4-23 significantly decreased portal pressure and tributary blood flow by 15% and 25% respectively but significantly increased portal territory vascular resistance by 30%. Systemic and renal haemodynamics were not altered by ANP4-23. In normal rats, ANP4-23 did not significantly change splanchnic, renal and systemic haemodynamics. In both groups of rats, ANP4-23 increased plasma ANP1-28 concentrations but did not change sodium excretion. CONCLUSIONS: ANP4-23 administration induced splanchnic vasoconstriction in cirrhotic but not in normal rats. ANP4-23-elicited vasoconstriction caused a portal hypotensive effect in cirrhotic rats. Finally, in both groups, ANP4-23 caused an increase in plasma ANP1-28 concentrations but did not increase sodium excretion.


Subject(s)
Atrial Natriuretic Factor/pharmacology , Hemodynamics/drug effects , Liver Cirrhosis, Experimental/physiopathology , Peptide Fragments/pharmacology , Splanchnic Circulation/drug effects , Animals , Atrial Natriuretic Factor/blood , Guanylate Cyclase/metabolism , Hemodynamics/physiology , Hypertension, Portal/chemically induced , Hypertension, Portal/physiopathology , Kidney/drug effects , Kidney/metabolism , Liver Cirrhosis, Experimental/drug therapy , Male , Rats , Rats, Sprague-Dawley , Receptors, Atrial Natriuretic Factor/metabolism , Sodium/urine , Splanchnic Circulation/physiology
5.
Cardiovasc Res ; 42(1): 87-98, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10434999

ABSTRACT

OBJECTIVE: To address the effect of longstanding left ventricular (LV) hypertrophy and failure on LV adenylyl cyclase (AC) gene expression, mRNA concentrations of the main cardiac AC isoforms were measured in the non-infarcted area of LV from rats with myocardial infarction (MI), without (H) or with (F) LV failure, and in control (C) rats. Basal, GTP- and forskolin-stimulated Mg(2+)- and Mn(2+)-dependent AC activities were also measured in F and C rats. METHODS: Two- and six months after MI, steady-state AC mRNA concentrations were assessed by Northern blot analysis and RNase protection assay with isoform-specific cDNA and cRNA probes, respectively. AC activities were assessed on LV microsomal fractions using standard procedures. RESULTS: Types V and VI, and types IV and VII were the major and minor AC mRNA isoforms in both the LVs of F and C rats. Two months after MI, no difference in LV type V or VI mRNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA ratios was observed in rats with H or F compared to C. Six months after MI, no difference in LV type V mRNA concentration was observed between the three rat groups, whether this level was normalized to GAPDH, poly-(A+) or 18S RNAs. In contrast, a 35% decrease in the type VI mRNA to poly-(A+) RNA ratio and a 29% decrease in the type VI mRNA to 18S RNA ratio was observed only in rats with F compared to C (p < 0.05 vs. C for the two comparisons). Two- and six months after MI, basal and forskolin-stimulated Mg(2+)-dependent AC activities were decreased by 30-35% in F rats compared to C (p < 0.05), whereas Mn(2+)-dependent activities were unchanged. CONCLUSION: Longstanding LV hypertrophy and failure resulting from MI in rats is not associated with altered expression of the most abundant, type V, AC mRNA isoform, whereas that of type VI is decreased. The lack of change in Mn(2+)-dependent AC activities in the LV of F rats suggests that this decrease has no functional consequence on overall AC activity and that decreased Mg(2+)-dependent activities are related to alterations occurring upstream.


Subject(s)
Adenylyl Cyclases/genetics , Heart Failure/etiology , Isoenzymes/genetics , Myocardial Infarction/complications , Myocardium/enzymology , RNA, Messenger/analysis , Adenylyl Cyclases/metabolism , Analysis of Variance , Animals , Blotting, Northern , Colforsin/pharmacology , Enzyme Activation , Gene Expression , Guanosine Triphosphate/pharmacology , Heart Failure/enzymology , Isoenzymes/metabolism , Magnesium/metabolism , Male , Manganese/metabolism , Microsomes/enzymology , Myocardial Infarction/enzymology , Rats , Rats, Wistar , Stimulation, Chemical , Time Factors
6.
Clin Pharmacol Ther ; 65(5): 500-10, 1999 May.
Article in English | MEDLINE | ID: mdl-10340915

ABSTRACT

BACKGROUND: Both malnutrition and malaria affect drug disposition and are frequent among children in the tropics. We assessed their respective influence on quinine distribution. METHODS: Forty children were divided into 4 groups: children with normal nutritional status without (group 1) or with (group 2) cerebral malaria, and malnourished children without (group 3) or with (group 4) cerebral malaria. All children received an infusion of 8 mg/kg of a combination solution of cinchona alkaloids that contained 96.1% quinine, 2.5% quinidine, 0.68% cinchonine, and 0.67% cinchonidine (corresponding to 4.7 mg/kg quinine base). The children with malaria then received repeated infusions every 8 hours for 3 days. Pharmacokinetic profiles of plasma and erythrocyte quinine were determined during the first 8 hours, together with quinine protein binding. Additional measurements of plasma quinine concentrations were used to simulate quinine concentrations profiles in children with malaria with and without malnutrition. Clinical recovery and parasitemia clearance times were determined in the children with malaria. RESULTS: Compared with control children, malaria and malnutrition increased plasma concentrations of quinine and reduced both the volume of distribution and the total plasma clearance. Simultaneously, alglycoprotein plasma concentrations and protein-bound fraction of the drug were increased. Erythrocyte quinine concentrations correlated strongly with free plasma quinine but not with the extent of parasitemia. Similar effective and nontoxic quinine concentration profiles were obtained in malaria with and without malnutrition. CONCLUSIONS: Severe global malnutrition and cerebral malaria have a similar effect on quinine pharmacokinetics in children. Moderate malnutrition does not potentiate cerebral malaria-mediated modifications of quinine disposition. These results suggest that current parenteral quinine regimens can be used, unmodified, to treat children with both malaria and malnutrition.


Subject(s)
Antimalarials/pharmacokinetics , Malaria, Cerebral/blood , Malaria, Cerebral/complications , Nutrition Disorders/blood , Nutrition Disorders/complications , Parasitemia/blood , Parasitemia/parasitology , Quinine/pharmacokinetics , Antimalarials/administration & dosage , Antimalarials/blood , Child , Child, Preschool , Drug Therapy, Combination , Erythrocytes/metabolism , Female , Humans , Infusions, Intravenous , Malaria, Cerebral/drug therapy , Male , Parasitemia/drug therapy , Quinine/administration & dosage , Quinine/blood
7.
Trans R Soc Trop Med Hyg ; 92(4): 437-40, 1998.
Article in English | MEDLINE | ID: mdl-9850403

ABSTRACT

The intrarectal route has been shown to be an alternative to parenteral therapy for the treatment of acute uncomplicated malaria. We conducted an open randomized clinical study of intrarectal Quinimax (a Cinchona alkaloids association) (20 mg/kg, then 15 mg/kg every 8 h) vs. intravenous Quinimax (8 mg/ kg infused over 4 h every 8 h) for 2 d in 76 children (39 in the intrarectal and 37 in the infusion groups) with cerebral falciparum malaria in Niger. This treatment was followed by oral chloroquine (10 mg/kg/d for 3 d). The primary end points of the study were fatal outcome and coma recovery time. In the intrarectal group, 35 children were cured (90%) and 4 died. In the infused group, 28 were cured (76%) and 9 died; mean coma recovery times were 34.6 h (SD = 12.8) and 33.0 h (SD = 14.1) for the intrarectal and infused groups, respectively. None of the differences was significant. Both treatments were well tolerated and no anal irritation was observed with intrarectal Quinimax. These findings suggest that intrarectal Quinimax can be an alternative to intravenous administration for rapid onset childhood cerebral malaria in the rural tropics, where the safety of parenteral administration cannot be guaranteed.


Subject(s)
Antimalarials/therapeutic use , Malaria, Cerebral/drug therapy , Administration, Rectal , Adolescent , Child , Child, Preschool , Chloroquine/therapeutic use , Cinchona Alkaloids/therapeutic use , Drug Combinations , Humans , Infusions, Parenteral/standards , Niger , Quinidine/therapeutic use , Quinine/therapeutic use , Treatment Outcome
8.
J Gastroenterol Hepatol ; 12(3): 233-6, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9142641

ABSTRACT

Little is known about the plasma concentrations of cyclic 3',5'-guanosine monophosphate (cGMP) in patients with cirrhosis. However, plasma cGMP concentrations provide information on cellular cGMP production by particulate guanylyl cyclases (which are stimulated by natriuretic peptides, such as atrial natriuretic peptide; ANP). In contrast, because intracellular cGMP elicits vasorelaxant mechanisms, plasma cGMP concentrations may be related to haemodynamic alterations in patients with cirrhosis. The aim of the present study was to measure plasma cGMP concentrations in patients with cirrhosis and controls and to examine the relationship between cGMP levels and plasma ANP concentrations and haemodynamic values. Plasma concentrations of cGMP and ANP and splanchnic and systemic haemodynamics were measured in 23 subjects; 13 subjects had cirrhosis and 10 were controls. All subjects had normal glomerular filtration. Plasma cGMP concentrations were significantly higher in patients (6.5 +/- 0.8 pmol/mL) than in controls (2.7 +/- 0.4 pmol/mL), while plasma ANP concentrations did not significantly differ between the two groups (127 +/- 22 and 123 +/- 27 pg/mL, respectively). In patients with cirrhosis, no significant correlation was found between plasma cGMP concentrations and plasma ANP concentrations, hepatic venous pressure gradient, cardiac output or systemic vascular resistance. In conclusion, in patients with cirrhosis, increased plasma cGMP concentrations may be due to an activation of particulate guanylyl cyclases by natriuretic peptides other than ANP. The present study suggest that plasma cGMP concentrations are not related to cirrhosis-induced haemodynamic alterations.


Subject(s)
Atrial Natriuretic Factor/blood , Cyclic GMP/blood , Hemodynamics , Liver Cirrhosis/blood , Liver Cirrhosis/physiopathology , Adult , Atrial Natriuretic Factor/urine , Cyclic GMP/urine , Female , Humans , Liver Cirrhosis/urine , Male , Middle Aged , Osmolar Concentration , Reference Values
9.
Circulation ; 94(6): 1316-24, 1996 Sep 15.
Article in English | MEDLINE | ID: mdl-8822986

ABSTRACT

BACKGROUND: Converting enzyme inhibitors meet most of the criteria required to be used in acute pulmonary edema. However, they could also induce deleterious effects on renal function and electrolytes. The purpose of this study was to evaluate the efficacy and safety of a single intravenous 2-hour infusion of enalaprilat (1 mg) after an acute pulmonary edema. METHODS AND RESULTS: This was a placebo-controlled, randomized, double-blind study performed in 20 congestive heart failure patients (New York Heart Association class III or IV). Systemic and regional hemodynamic parameters, biological parameters, and blood gases were measured before and repeatedly after the onset of infusion. Compared with placebo, enalaprilat decreased pulmonary capillary wedge pressure (-37% versus -10%, P = .001), diastolic and mean systemic blood pressures (-21% versus 0%, P = .009, and -18% versus -1%, P = .026, respectively), diastolic and mean pulmonary blood pressures (-21% versus -8%, P = .040; -18% versus -9%, P = .046), and brachial and renal resistances (-44% versus -14%, P = .017, and -22% versus -2%, P = .014, respectively); increased brachial and renal blood flows (+77% versus +8%, P = .036, and +12% versus 0%, P = .043, respectively), arterial oxygen tension (+2% versus -16%, P = .041), and arterial oxygen saturation (+1% versus -2%, P = .045); and tended to decrease rate-pressure product (-19% versus -7%, P = .076), increase brachial artery diameter (+13% versus 0%, P = .081), and improve intrapulmonary shunt (-18% versus +16%, P = .080). Enalaprilat did not affect cardiac output or carotid or hepatosplanchnic hemodynamics. CONCLUSIONS: Early administration of enalaprilat is effective and well tolerated in acute pulmonary edema.


Subject(s)
Enalaprilat/administration & dosage , Heart Failure/complications , Pulmonary Edema/drug therapy , Pulmonary Edema/etiology , Acute Disease , Aged , Double-Blind Method , Enalaprilat/adverse effects , Enalaprilat/therapeutic use , Female , Gases/blood , Heart Failure/physiopathology , Hemodynamics/drug effects , Hormones/blood , Humans , Injections, Intravenous , Male , Placebos , Pulmonary Circulation/drug effects , Pulmonary Edema/physiopathology
10.
Eur J Pharmacol ; 312(1): 63-8, 1996 Sep 19.
Article in English | MEDLINE | ID: mdl-8891579

ABSTRACT

In portal hypertension, the role of the vasorelaxant nitric oxide (NO) in long-term splanchnic and systemic vascular tone regulation is unclear. This study examined the effects of long-term administration of a NO synthesis inhibitor on haemodynamics in portal hypertensive rats. Rats were randomly assigned to receive either water (placebo) or 100 mg/kg.day of oral N-nitro-L-arginine methylester (L-NAME) for 28 days. At 14 days, the portal vein was ligated in 10 rats from each group. At 28 days, splanchnic and systemic blood flows were measured in 20 normal and 20 portal vein stenosed rats. Plasma atrial natriuretic peptide (ANP) concentrations as well as plasma and urinary cyclic guanosine monophosphate (cGMP) levels were also measured. Porto-systemic shunts were measured in other portal vein stenosed animals that had or had not received L-NAME. Portal vein stenosed rats that received L-NAME had significantly lower portal tributary blood flow and percentages of portal-systemic shunting (7.3 +/- 0.5 versus 3.7 +/- 0.2 ml/min.100 g and 96 +/- 1 versus 68 +/- 5%, respectively) and higher hepatocollateral vascular resistance (147 +/- 10 versus 295 +/- 30 dyn.s.cm-5.100 g.10(3), respectively) than placebo portal vein stenosed rats. Portal pressure, ANP and cGMP levels did not differ between the groups. Arterial pressure was significantly higher and cardiac index lower after L-NAME than after placebo. Normal rats had similar but less marked L-NAME-induced responses than portal hypertensive rats. The presence of a long-term L-NAME-induced vasoconstriction in collateral vessels and splanchnic and systemic arterioles in portal vein stenosed rats indicates that a NO-mediated vasodilator tone may contribute to the development and the maintenance of collateral circulation as well as splanchnic and systemic vasodilation in portal hypertension. Moreover, the NO-mediated vasodilator tone in portal hypertensive animals seems to be increased.


Subject(s)
Enzyme Inhibitors/pharmacology , Hemodynamics/drug effects , Hypertension, Portal/physiopathology , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Animals , Atrial Natriuretic Factor/blood , Cyclic GMP/blood , Male , Rats , Rats, Sprague-Dawley , Vasoconstriction/drug effects
11.
Clin Chem ; 42(7): 1086-91, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8674193

ABSTRACT

We describe an isocratic liquid-chromatographic method with amperometric detection for determination of serotonin by rapid sample preparation. Platelet-poor plasma and platelets were injected after a single deproteinization step with perchloric acid. Addition of sodium borohydride to whole blood avoids oxidation of serotonin during the deproteinization step without any chromatographic interferences. We purified urinary serotonin by two successive cationic and anionic extraction steps. After urine dilution, urinary 5-hydroxyindoleacetic acid (5-HIAA) was measured under the same chromatographic conditions. Platelet serotonin concentrations correlated more closely with whole-blood serotonin concentrations after correction for platelet number than with concentrations expressed in nmol/L. This suggests that whole-blood serotonin measurements should be corrected for platelet count to eliminate the variability of circulating platelets. Combined determination of serotonin in whole blood and urine and of 5-HIAA in urine provides a useful tool for detecting and monitoring carcinoid tumors.


Subject(s)
Blood Platelets/chemistry , Chromatography, High Pressure Liquid/methods , Serotonin/blood , Serotonin/urine , Adult , Aged , Anions , Borohydrides , Bronchial Neoplasms/blood , Bronchial Neoplasms/urine , Carcinoid Tumor/blood , Carcinoid Tumor/urine , Cations , Chromatography, High Pressure Liquid/statistics & numerical data , Humans , Hydroxyindoleacetic Acid/urine , Ileal Neoplasms/blood , Ileal Neoplasms/urine , Middle Aged , Oxidation-Reduction , Pancreatic Neoplasms/blood , Pancreatic Neoplasms/urine , Platelet Count , Reference Values , Sensitivity and Specificity , Stomach Neoplasms/blood , Stomach Neoplasms/urine
12.
Br J Clin Pharmacol ; 41(5): 389-95, 1996 May.
Article in English | MEDLINE | ID: mdl-8735679

ABSTRACT

1. Three groups of seven children aged 2-14 years with acute uncomplicated Plasmodium falciparum malaria received 12.8 mg kg-1 quinine gluconate by the intrarectal route (new cream formulation) or 8 mg kg-1 Quinimax (a Cinchona alkaloid alkaloid combination) by the intramuscular or intravenous (4 h infusion) route every 8 h for 3 days. Clinical and parasitological status was similar in the three groups at enrolment. 2. At 36 h, body temperature of all children of the three groups was returned to normal and remained so until day 7. 3. The decrease in parasitaemia did not differ between the three groups and the time required for a 50% fall in parasitaemia relative to baseline was 12.3 +/- 5.4, 18.2 +/- 6.1 and 14.5 +/- 4.2 h in the intrarectal, intramuscular and intravenous treatment groups, respectively. Parasitaemia expressed as a percentage of initial values was not significantly different in the three groups after 48 h of treatment (7.4 +/- 16.0, 4.1 +/- 4.2 and 2.2 +/- 3.8% in the intrarectal, intramuscular and intravenous treatment groups, respectively). All the patients were aparasitaemic by day 7. 4. The tolerability of the three treatments was good; in particular, no rectal irritation was reported with the cream formulation. 5. The tmax occurred later after intrarectal (4.1 +/- 2.4 h) and intravenous infusion (3.8 +/- 0.5 h) than after intramuscular injection (1.6 +/- 1.3 h) (P = 0.02). Cmax was lower with the intrarectal (3.0 +/- 1.0 mg 1(-1)) and intramuscular routes (3.2 +/- 0.7 mg 1(-1)) than with the intravenous route (5.1 +/- 1.4 mg 1(-1)) (P = 0.003). Areas under the curve (AUC(0, 8 h)) were smaller with intrarectal (17.0 +/- 7 mg 1(-1) h) and intramuscular routes (19.4 +/- 4.8 mg 1(-1)) than with the intravenous route (27.8 +/- 8.2 mg 1(-1) h) (P = 0.02). The approximate bioavailability of intrarectal quinine from 0 to 8 h was 36% vs intravenous quinine and 51% vs intramuscular quinine. 6. The good tolerability and efficacy of this new intrarectal quinine formulation outweigh its low approximate bioavailability. This new approach might thus be a safe and effective alternative to intramuscular quinine injection for the treatment of children with acute uncomplicated Plasmodium falciparum malaria in the field.


Subject(s)
Antimalarials/pharmacokinetics , Malaria, Falciparum/drug therapy , Quinine/pharmacokinetics , Administration, Rectal , Adolescent , Antimalarials/administration & dosage , Antimalarials/blood , Child , Child, Preschool , Humans , Infusions, Intravenous , Injections, Intramuscular , Malaria, Falciparum/blood , Niger , Quinine/administration & dosage , Quinine/blood
13.
Fundam Clin Pharmacol ; 10(2): 127-35, 1996.
Article in English | MEDLINE | ID: mdl-8737955

ABSTRACT

The effects of a single oral dose (6 mg) of the angiotensin-I converting enzyme inhibitor, spirapril, on systemic, pulmonary and regional (brachial, renal, hepato-splanchnic) hemodynamics as well as on biological parameters investigating the renin-angiotensin-aldosterone and sympathetic nervous systems were studied over a 24-hour period in eight patients with severe congestive heart failure (CHF). As compared to pretreatment values, spirapril significantly decreased mean arterial (-19%, peak effect), right atrial (-42%), mean pulmonary arterial (-38%) and pulmonary capillary wedge (-46%) pressures. Spirapril significantly decreased heart rate (-14%) and increased stroke volume index (+43%) thus resulting in a slight increase in cardiac index. All these effects were maximal between 2.5 and 4 h. Brachial artery diameter (+12%) and brachial (+41%) and renal (+36%) blood flows increased significantly whereas brachial (-41%) and renal (-36%) vascular resistances decreased significantly. All these effects were usually maximal between 1 and 2.5 h. Hepato-splanchnic hemodynamics were not drug-affected. Spirapril significantly inhibited plasma converting enzyme activity (-96% at 4 h), increased plasma renin activity (+505% at 4 h), and decreased plasma aldosterone (-46% at 24 h), norepinephrine (-31% at 24 h) and atrial natriuretic factor (-33% at 7 h). Thus, in severe CHF, acute administration of spirapril, 6 mg orally, exerts both arterial and venous vasodilating properties and improves both the systemic and regional hemodynamics and the biological status of the patients.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/therapeutic use , Enalapril/analogs & derivatives , Heart Failure/drug therapy , Hemodynamics/drug effects , Aged , Blood Pressure/drug effects , Brachial Artery/drug effects , Brachial Artery/physiology , Enalapril/pharmacokinetics , Enalapril/therapeutic use , Female , Heart Failure/physiopathology , Humans , Liver Circulation/drug effects , Male , Middle Aged , Pulmonary Circulation/drug effects , Pulmonary Wedge Pressure/drug effects , Renal Circulation/drug effects , Vascular Resistance/drug effects , Vasodilation/drug effects
14.
Br J Clin Pharmacol ; 40(5): 431-8, 1995 Nov.
Article in English | MEDLINE | ID: mdl-8703646

ABSTRACT

1. The effects of a single oral dose (50 mg) of the angiotensin II AT1-receptor antagonist, losartan, on the systemic and regional vascular responses to simulated orthostatic stress by the lower body negative pressure (LBNP) technique were investigated in nine healthy volunteers, in a double-blind, placebo-controlled crossover study. 2. Arterial blood pressure remained unchanged throughout the study. Three hours after its administration and before LBNP, losartan selectively increased renal blood flow (PAH clearance) by 8.3% (3.5 to 13.1%, 95% CI) from 1.25 +/- 0.08 l min-1 (P < 0.05) and decreased plasma aldosterone levels by 58% (29 to 87%, 95% CI) from 22 +/- 3 ng 100 ml-1 (P < 0.05). 3. LBNP at -10 and -20 mm Hg induced a progressive and significant decrease in central venous pressure and increases in forearm (plethysmography) and splanchnic (indocyanine green clearance) vascular resistances which were similar after losartan and placebo administrations. Losartan blunted the LBNP-induced increase in renal vascular resistance observed at -20 mm Hg after placebo but a similar increase in glomerular filtration rate (inulin clearance) was observed at LBNP -10 and -20 mm Hg after losartan and placebo. Calculated filtration fraction increased after placebo (LBNP -10 mm Hg) and losartan (LBNP -20 mm Hg). Finally, LBNP-induced changes in biological parameters were similar after losartan and placebo at all levels of LBNP. 4. Thus, losartan does not interfere with the adaptive forearm and splanchnic vascular responses and preserves renal haemodynamics during orthostatic stress simulated by LBNP in healthy volunteers.


Subject(s)
Angiotensin Receptor Antagonists , Biphenyl Compounds/pharmacology , Forearm/blood supply , Imidazoles/pharmacology , Renal Circulation/drug effects , Splanchnic Circulation/drug effects , Tetrazoles/pharmacology , Vasoconstriction/drug effects , Adult , Baroreflex/drug effects , Double-Blind Method , Hemodynamics/drug effects , Humans , Losartan , Lower Body Negative Pressure , Male
15.
Trans R Soc Trop Med Hyg ; 89(4): 418-21, 1995.
Article in English | MEDLINE | ID: mdl-7570885

ABSTRACT

In an attempt to avoid the complications associated with intramuscular quinine administration, we assessed the intrarectal route. Sixty-six children aged from 2 to 10 years with Plasmodium falciparum malaria were included in the study, which took place in Niamey, Niger. Fifty-five children were given 20 mg/kg of the diluted injectable form of Quinimax (a quinine, quinidine, cinchonine, cinchonidine association) intrarectally. A further 11 children with malaria were treated with 12.5 mg/kg of the same Quinimax solution by the intramuscular route. All the children were treated twice a day for 3 d. Blood samples were drawn from 20 children (15 treated intrarectally and 5 intramuscularly) for a kinetic study. Both modes of administration were well tolerated. Mean fever clearance times (+/- standard errors) were 48.6 +/- 2.7 h and 35.9 +/- 2.2 h in the intrarectal and intramuscular groups, respectively (P = 0.05). Mean parasite clearance times (+/- standard errors) and mean times to achieve 50% reduction in parasitaemia (+/- standard errors) were similar after intrarectal (46.5 +/- 5.7 h and 7.8 +/- 0.9 h respectively) and intramuscular administration (27.4 +/- 3.6 h and 8.7 +/- 1.7 h, respectively). Tmax. after intrarectal administration (2.7 +/- 0.4 h) did not differ significantly from the value after intramuscular administration (1.1 +/- 0.6 h), but Cmax. and the area under the concentration-time curve from 0 to 48 h were lower (4.9 +/- 0.6 mg/L and 230.0 +/- 9.6 mg/L.h, respectively) than after intramuscular administration (9.1 +/- 1.2 mg/L and 356.0 +/- 4.2 mg/L.h, respectively) (P < 0.001). Compared to the intramuscular route, intrarectal Quinimax bioavailability was 40%.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Antimalarials/therapeutic use , Cinchona Alkaloids/therapeutic use , Malaria, Falciparum/drug therapy , Quinine/therapeutic use , Administration, Rectal , Antimalarials/pharmacokinetics , Child , Child, Preschool , Cinchona Alkaloids/pharmacokinetics , Drug Combinations , Humans , Injections, Intramuscular , Niger , Quinine/pharmacokinetics
16.
Fundam Clin Pharmacol ; 9(5): 479-87, 1995.
Article in English | MEDLINE | ID: mdl-8617412

ABSTRACT

The effects of a single oral dose (5 mg) of the angiotensin-converting enzyme (ACE) inhibitor, ramipril, on the systemic and regional vascular responses to simulated orthostatic stress by the lower body negative pressure (LBNP) technique were investigated in eight healthy volunteers, in a double-blind, placebo-controlled crossover study. Arterial blood pressure remained unchanged throughout the study. Ramipril increased significantly forearm (venous occlusion plethysmography, + 37% +/- 4% from 1.98 ml.min-1.100 ml-1) and renal (PAH clearance, + 6 +/- 2% from 1.20 1.min-1) blood flows and decreased corresponding vascular resistances, 150 minutes after its administration and before LBNP. It also significantly reduced calculated filtration fraction and inhibited by approximately 86% plasma ACE activity. Lower body negative pressure at -10 and -20 mmHg induced a progressive and significant decrease in central venous pressure and significant increases in forearm, splanchnic (indocyanine green clearance) and total peripheral vascular resistances which were of the same magnitude after ramipril and placebo administrations. Ramipril blunted the LBNP-induced increase in renal vascular resistance observed at -20 mmHg after placebo but a similar increase in glomerular filtration rate (inulin clearance) was observed at LBNP-10 and -20 mmHg after ramipril and placebo. Calculated filtration fraction increased after placebo (LBNP-10 mmHg) and ramipril (LBNP-20 mmHg). Finally, LBNP-induced changes in biological parameters were similar after ramipril and placebo at all levels of LBNP. Thus, ramipril does not interfere with the adaptive forearm and splanchnic vascular responses and preserves renal hemodynamics during orthostatic stress simulated by LBNP in healthy volunteers.


Subject(s)
Blood Flow Velocity/drug effects , Blood Pressure/drug effects , Hemodynamics/drug effects , Ramipril/pharmacology , Adult , Angiotensin-Converting Enzyme Inhibitors/pharmacology , Epinephrine/blood , Humans , Male , Vasopressins/blood
17.
Med Trop (Mars) ; 55(4 Suppl): 27-32, 1995.
Article in French | MEDLINE | ID: mdl-8649262

ABSTRACT

Modulating agents that circumvent the Plasmodium falciparum resistance to antimalarials are components without any intrinsic antimalarial activity but able, in association with a standard antimalarial drug, to restore the sensitivity of Plasmodium falciparum to this drug. Two hypotheses are forwarded about their possible mechanisms of action : the mechanisms involving the inhibition of the drug efflux and the mechanisms involving the inhibition of antimalarial drug metabolic degradation. None of these mechanisms is able to alone explain the effect of all modulating agents. The future use prospects of these compounds are limited by their in vivo toxicity. That relevant to the calcium channels blockers seems to be circumvented by the use of dextro-enantiomers. A toxicity relevant to a possible accumulation of the antimalarial in healthy, non target cells must be also suspected. Moreover the use of these agents evidences a socio-economic problem : a complete new development of the association (antimalaria plus modulatin drugs) is needed and that, in developing countries. Nevertheless, the agents modulating the Plasmodium falciparum resistance are a new approach for the treatment of resistant malaria which might give a recrudescence of activity to the old antimalarials as chloroquine.


Subject(s)
Antimalarials/therapeutic use , Calcium Channel Blockers/therapeutic use , Drug Resistance , Malaria, Falciparum/drug therapy , Animals , Antimalarials/chemistry , Antimalarials/metabolism , Calcium Channel Blockers/pharmacology , Drug Synergism , Drug Therapy, Combination , Humans , Malaria, Falciparum/parasitology
18.
J Antimicrob Chemother ; 34(5): 803-8, 1994 Nov.
Article in English | MEDLINE | ID: mdl-7706177

ABSTRACT

Twelve healthy volunteers received two doses of amopyroquin 6 mg/kg im 24 h apart. Blood and plasma concentrations of amopyroquin and two metabolites were assayed by HPLC from 0 to 48 h. Half-life values for amopyroquin, calculated from 0 to 48 h whole-blood and plasma samples were 13.9 +/- 9.1 and 18.3 +/- 6.8 h respectively. Two metabolites were detected in blood and they had very low in-vitro activity against Plasmodium falciparum compared with the parent drug. Neither hypotension nor lengthening of QRS complex were observed in any volunteers and hepatic enzymes remained in the normal range despite a transitory increase. These results confirm that unchanged amopyroquin accounts for antimalarial activity in vivo and that two doses of 6 mg/kg are well tolerated.


Subject(s)
Aminoquinolines/pharmacokinetics , Antimalarials/pharmacokinetics , Adult , Aminoquinolines/adverse effects , Aminoquinolines/pharmacology , Animals , Half-Life , Humans , Injections, Intramuscular , Male , Plasmodium falciparum/drug effects
19.
Diabetologia ; 37(9): 879-84, 1994 Sep.
Article in English | MEDLINE | ID: mdl-7528695

ABSTRACT

Nitric oxide, which is produced from L-ar-ginine by a nitric oxide-synthase enzyme, has been shown to be a ubiquitous messenger molecule. Recently, it has been suggested that nitric oxide might influence insulin secretion by activating the soluble guanylate cyclase and generating cyclic guanosine monophosphate (cGMP). We have investigated the role of the nitric oxide pathway in insulin secretion by evaluating the insulin response to several secretagogues in rats in which nitric oxide-synthase was chronically inhibited by oral administration of the L-arginine analogue, NG-nitro-L-arginine methyl ester (L-NAME). Blood pressure and aortic wall cGMP content were used as indices of nitric oxide-synthase blockade. Insulin secretion was evaluated after an intravenous bolus of D-glucose, L-arginine or D-arginine. Chronic L-NAME administration induced a 30% increase in blood pressure and a seven-fold drop in arterial cGMP content. Body weight, fasting plasma glucose and insulin were not influenced by L-NAME administration. First-phase insulin secretion (1 + 3 min) in response to glucose was not significantly different in L-NAME and control rats. The areas under the insulin curve were similar in both groups. Insulin secretion in response to D-arginine or L-arginine in L-NAME-treated and control rats were also similar. In conclusion, chronic nitric oxide-synthase blockade increases blood pressure and decreases aortic cGMP content, but does not alter insulin secretion in response to several secretagogues. Chronic oral administration of L-NAME in the rat provides an adequate animal model for studying the L-arginine nitric oxide-pathway.


Subject(s)
Amino Acid Oxidoreductases/antagonists & inhibitors , Arginine/analogs & derivatives , Insulin/metabolism , Animals , Aorta, Thoracic/metabolism , Arginine/pharmacology , Blood Glucose/drug effects , Blood Glucose/metabolism , Blood Pressure/drug effects , Body Weight/drug effects , Cyclic GMP/metabolism , Epinephrine/blood , Heart Rate/drug effects , Insulin/blood , Insulin Secretion , Male , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/metabolism , NG-Nitroarginine Methyl Ester , Nitric Oxide Synthase , Norepinephrine/blood , Rats , Rats, Wistar , Reference Values , Time Factors
20.
J Cardiovasc Pharmacol ; 24(3): 500-8, 1994 Sep.
Article in English | MEDLINE | ID: mdl-7528307

ABSTRACT

The effects of single oral doses of lisinopril (5 and 20 mg) on systemic and regional hemodynamics were investigated noninvasively in a placebo-controlled, randomized, double-blind, cross-over study of 6 healthy male volunteers. Lisinopril induced a dose-dependent (significant after 20 mg) and long-lasting (< or = 8 h) decrease in mean arterial pressure (MAP, approximately 11% after 20 mg) that was related to a decrease in total peripheral resistance (TPR), because simultaneously heart rate (HR) and cardiac output (CO) were unchanged. Brachial artery flow (+42 and +47% after 5 and 20 mg, respectively) and diameter (+8 and +9%) increased significantly, whereas brachial vascular resistance (-31 and -38%) decreased significantly from 2 to 8 h after drug intake. Common carotid artery flow (+20 and +24%) also increased significantly, whereas corresponding resistance (-18 and -26%) decreased significantly during the same period. Finally, CO was significantly redistributed toward the brachial and, to a lesser extent, the carotid vascular beds after both doses of lisinopril. We conclude that in healthy subjects lisinopril, at non- or slightly hypotensive doses, dilates both arterioles and large arteries and that this vasodilation is not homogeneous, affecting preferentially the brachial rather than the carotid vascular bed.


Subject(s)
Arteries/drug effects , Arterioles/drug effects , Hemodynamics/drug effects , Lisinopril/pharmacology , Vasodilation/drug effects , Administration, Oral , Adult , Analysis of Variance , Arteries/physiology , Arterioles/physiology , Blood Pressure/drug effects , Brachial Artery/drug effects , Brachial Artery/metabolism , Brachial Artery/physiology , Cardiac Output/drug effects , Carotid Arteries/drug effects , Carotid Arteries/metabolism , Carotid Arteries/physiology , Cross-Over Studies , Dose-Response Relationship, Drug , Double-Blind Method , Drug Evaluation , Heart Rate/drug effects , Humans , Laser-Doppler Flowmetry , Lisinopril/administration & dosage , Lisinopril/pharmacokinetics , Male , Regional Blood Flow/drug effects , Vascular Resistance/drug effects
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