ABSTRACT
Expression of Bcl2 family genes was studied during the early phase of long-term potentiation in the CA1 field of rat hippocampal slices. The level of Bax mRNA and protein increased, while the content of Bcl2 mRNA and protein decreased 30 min after tetanization of the Schaffer collaterals. Our results suggest that proteins of the Bcl2 family play a role in the mechanisms of synaptic plasticity.
Subject(s)
CA1 Region, Hippocampal/physiology , Long-Term Potentiation , bcl-2-Associated X Protein/metabolism , Animals , Gene Expression , Male , Rats, Wistar , bcl-2-Associated X Protein/geneticsABSTRACT
The expression of egr-1, junB, c-jun, and c-fos genes in rat hippocampal CA1 field was studied by the real-time PCR 30, 60, and 120 min after induction of long-term posttetanic potentiation. The content of egr-1, junB, and c-jun mRNA gradually increased and doubled 120 min after tetanization. The increase in c-jun mRNA level lagged behind the increment of egr-1 and junB. The level of c-fos mRNA increased 30 min after tetanization, returned to the initial level by min 60, and again increased 120 min after induction of long-term posttetanic potentiation.
Subject(s)
Hippocampus/metabolism , Long-Term Potentiation/physiology , Animals , Early Growth Response Protein 1/genetics , Long-Term Potentiation/genetics , Polymerase Chain Reaction , Proto-Oncogene Proteins c-fos/genetics , Proto-Oncogene Proteins c-jun/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , RatsABSTRACT
We studied the effect of triphenyldioxane on phase I xenobiotic metabolism enzymes in the liver of rats and rabbits. Total cytochrome P450 content, protein concentration, and catalytic activity of CYP2B, CYP3A, and CYP2C isoforms were measured. Triphenyldioxane significantly increases specific activity of CYP2B and CYP2C in the liver of rats and rabbits, respectively. Immunoblotting analysis of microsomal enzymes in the liver of animals showed that the increase in specific activity of CYP is related to high content of apoenzymes. We showed for the first time that rats and rabbits are characterized by interspecies differences in the induction of cytochrome P450 isoforms under the influence of triphenyldioxane.
