ABSTRACT
Background: The most common bacteria causing periodontitis were Porphyromonas gingivalis, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans. At present, plants are considered a valuable source of natural materials for use in the development of antimicrobial, anti-inflammatory and antioxidant agents. Hylocereus plyrhizus or red dragon fruit peel extract (RDFPE) contains terpenoids, and flavonoids can be an alternative. The gingival patch (GP) has been designed to ensure drug delivery and absorption into tissue targets. Objective: To assess the effect of a mucoadhesive gingival patch containing nano-emulsion of red dragon fruit peel extract (GP-nRDFPE) in inhibiting Porphyromonas gingivalis, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans as compared to control groups. Material and method: Inhibition by diffusion method was carried out in Porphyromonas gingivalis, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans medium. The test material was gingival patch mucoadhesive containing nano-emulsion red dragon fruit peel extract (GP-nRDFPR), gingival patch mucoadhesive containing red dragon fruit peel extract (GP-RDFPE), gingival patch mucoadhesive containing doxycycline (GP-dcx) and blank gingival patch (GP), conducted in four replications. The differences in the inhibition were analyzed using ANOVA and post hoc tests (p < 0.05). Result: GP-nRDFPE showed higher inhibition in Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum compared to GP-RDFPE at the concentrations 3.125% and 6.25% (p < 0.05). Conclusion: The GP-nRDFPE showed better anti-periodontic bacteria to Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans in line with its concentration. This is assumed that the GP-nRDFPE can use as periodontitis treatment.
ABSTRACT
OBJECTIVES: Diabetes mellitus (DM) is a major risk factor for periodontitis. Susceptibility to periodontitis increases approximately three times in people with DM. There is a clear relationship between the degree of hyperglycemia and the severity of periodontitis. This study aimed to analyze the reduction of tumor necrosis factor-α (TNF-α) in diabetics who came for periodontitis examination to prevent exacerbations. MATERIALS AND METHODS: This was an analytic observational study using a cross-sectional approach at health centers in Surabaya, Indonesia. Measurement of periodontal status used the community periodontal index of treatment needs by measuring bleeding at probing and pocket depth. TNF-α was measured using enzyme-linked immunosorbent assay, and behavior and lifestyle using a questionnaire. STATISTICAL ANALYSIS: The Kolmogorov-Smirnov test was performed to identify data normality (p < 0.05). A nonparametric test was used to measure the degree of association between different characteristics and the incidence of periodontitis in type 2 DM patients with and without periodontitis. Spearman's test was done to examine the correlation between TNF-α level and severity of periodontitis in diabetics. The significant level was at p <0.05. RESULTS: There was a correlation between age, predisposing factors, reinforcing factors, drug consumption, and TNF-α levels in patients with type 2 DM and the incidence of periodontitis. CONCLUSIONS: Poor glycemic control can induce oxidative stress on the gingiva, thereby aggravating damage to periodontal tissue. An important factor in preventing periodontitis for type 2 DM patients is controlling blood sugar levels through regular consumption of drugs and regular maintenance of oral cavity health. Knowledge is a predisposing factor that affects adherence of people with type 2 DM to consuming drugs regularly, which can be strengthened by family support. These will ultimately play a role in reducing TNF-α levels.
ABSTRACT
OBJECTIVE: Lactobacillus plantarum, a bacterium located in deep caries, has a virulence factor in the form of lipoteichoic acid (LTA), which is found in the bacterial cell wall. LTA is able to trigger a neutrophils response in the dental pulp inflammation process within the first 6 to 24 hours. The quantity of bacteria is one factor influencing the increase in number of neutrophils in addition to the quality of the bacteria. This study seeks to analyze the effect of lipoteichoic acid of Lactobacillus plantarum (LTA-Lp) in the dental pulp inflammation by observing the number of neutrophil cells in a histopathological view. MATERIALS AND METHODS: The LTA was isolated from L. plantarum. The left upper molar of Rattus novergicus was mechanically perforated under anesthesia to induce dental pulp inflammation. The perforated tooth was then induced by 10 and 15 µg/mL of LTA-Lp and then restored by a temporary filling. The perforated tooth in the control group was only restored by a temporary filling. After 24, 48, and 72 hours, the tooth was extracted and then stained with hematoxylins and eosin to observe the neutrophils in the dental pulp via a light microscope. RESULT: The number of neutrophils in the dental pulp after induction by 15 µg/mL of LTA-Lp is higher than 10 µg/mL of LTA-Lp and both controls. There were significant differences in the number of neutrophils in the dental pulp, in each group on 24, 48, and 72 hours after LTA-Lp inducing (p < 0.05). CONCLUSION: The LTA-Lp dose of of 10 and 15 µg/mL affected the dental pulp inflammation by affecting the number of neutrophils.
