ABSTRACT
There is a rather wide range of meanings for placebo and placebo effect, and some of the controversy has arisen when the proponents of various positions have ignored each other. An attempt is made to clarify some of the conceptual issues related to these concepts. Five uses of placebos as inert substances or treatments are listed. The problem of the placebo effect and the discussion of its existence are examined, and other terms that could cover the phenomenon are suggested. It is suggested that the concept placebo is appropriate only to the research context and that the term care effect be introduced to replace it in clinical contexts.
ABSTRACT
OBJECTIVE: Glucocorticoid receptor interacting protein 1 (GRIP1) is a coactivator that binds to the nuclear hormone receptors in a ligand-dependent manner and mediates transcriptional activation of the target genes. The aim of this study was to investigate GRIP1 expression in various murine tissues and whether the protein is nuclear, cytoplasmic, or both. DESIGN: Two novel polyclonal antibodies against amino acids 34-47 and 468-481 of GRIP1 were raised and characterized in order to study the GRIP1 expression with immunohistochemistry. RESULTS: Transient transfection studies with COS cells showed a clearly nuclear staining pattern and also immunohistochemical localization of GRIP1 was mainly nuclear, but cytoplasmic expression was seen as well. GRIP1 was expressed in epithelial cells of the submandibular gland, gastrointestinal tract, pancreas, kidney, uterus, mammary gland, testis, prostate, trachea, lungs and adrenal gland. GRIP1 was also detected in stromal cells of colon, rectum, urinary bladder, vagina, uterus, mammary gland and trachea, and to a lesser extent in esophagus, ureter, urethra, thymus and spleen. Smooth muscle cells of the gastrointestinal and urinary tract, uterus, epididymis, prostrate and bronchioles expressed GRIP1. Blood vessels of many organs, capsule of the kidney and prostate, mesovarium, adipocytes of the mammary gland, pericardium and cartilage of the trachea were also GRIP1-positive. Liver, thyroid gland and striated muscle did not express GRIP1. CONCLUSIONS: GRIP1 was expressed in a wide variety of murine organs, and expression varied between cell types and organs. In addition to mainly nuclear localization of endogenous GRIP1, cytoplasmic expression was seen as well.
Subject(s)
Antibodies , Immunohistochemistry , Transcription Factors/analysis , Animals , Antibodies/immunology , Antibody Specificity , COS Cells , Cardiovascular System/chemistry , Cell Nucleus/chemistry , Cytosol/chemistry , Digestive System/chemistry , Endocrine Glands/chemistry , Epithelial Cells/chemistry , Female , Immunoblotting , Mice , Muscle, Smooth/chemistry , Nuclear Receptor Coactivator 2 , Organ Specificity , Peptide Fragments/immunology , Respiratory System/chemistry , Tissue Distribution , Transcription Factors/genetics , Transcription Factors/immunology , Transfection , Urogenital System/chemistrySubject(s)
Ethics, Medical , Genetics, Medical , Holistic Health , Humans , Physician-Patient RelationsABSTRACT
Two novel antibodies against the mammalian progesterone receptor (PR) were raised and characterized to study the distribution of PR and the effect of estrogen on PR expression in various female murine tissues by immunohistochemistry. There were estrogen-independent constitutive PR expressions in the smooth muscle cells of uterus, uterine blood vessels, urinary bladder, duodenum, and jejunum of ovariectomized mice. Uterine stromal cells, capsular cells of kidney and adrenal gland, and the epithelial cells of submandibular gland expressed PR constitutively. PR expression was detected in some thymic cells and the number of PR-positive thymic cells increased markedly after estrogen treatment. Estrogen induced PR expression in the epithelial cells of uterus, vagina, urethra, and skin and the stromal cells of vagina, urethra, and pancreatic ducts, as well as the smooth muscle cells of some blood vessels. These results suggest cell-specific progesterone actions in the urinary tract, skin, and gastrointestinal organs, on the immune functions, and on the regulation of local blood flow.
Subject(s)
Receptors, Progesterone/analysis , Animals , Blood Vessels/chemistry , COS Cells , Digestive System/chemistry , Epithelial Cells/chemistry , Female , Gene Expression , Genitalia, Female/chemistry , Immunoblotting , Immunohistochemistry , Lymphoid Tissue/chemistry , Mice , Muscle, Smooth/chemistry , Peptide Fragments/immunology , Receptors, Progesterone/immunology , Respiratory System/chemistry , Stromal Cells/chemistry , Tissue Distribution , TransfectionABSTRACT
Doctor-patient interaction has gained increasing attention among sociologists and linguists during the last few decades. The problem with the studies performed so far, however, has been a lack of a theoretical framework which could bring together the various phenomena observed within medical consultations. Mikhail Bakhtin's philosophy of language offers us tools for studying medical practice as socio-cultural semiotic phenomenon. Applying Bakhtin's ideas of polyphonic, context-dependent and open-ended nature of human communication opens the possibilities to develop prevailing theoretical and empirical approaches to the study of medical consultations.
