ABSTRACT
Currently, special emphasis is being given to the design and fabrication of antibacterial nanocomposite hydrogels for wound dressing applications. Herein, we report the synthesis and characterization of hydroxypropyl methylcellulose (HPMC) reinforced with HPMC capped copper nanoparticles (HCu NPs) based nanocomposite hydrogel films (NHFs). Spherical nanostructures of HCu NPs (â¼40 nm) were achieved by facile precipitation technique using ascorbic acid as a nucleating agent and subsequently made their NHFs via solution casting method. Spectral, thermal and structural characteristics of the developed materials were carried out. Antibacterial activity of the resultant NHFs showed the MIC and MBC values of 350 and 1400 µg/mL for S. aureus, and 500 and 2000 µg/mL for E. coli, respectively. These results conveyed that the HCu NPs incorporated HPMC NHFs can be used effectively in antibacterial applications.
Subject(s)
Anti-Bacterial Agents/chemistry , Copper/chemistry , Hydrogels/chemistry , Hypromellose Derivatives/chemistry , Metal Nanoparticles/chemistry , Methylgalactosides/chemistry , Nanocomposites/chemistry , Ascorbic Acid/chemistry , Bandages , Chemical Precipitation , Escherichia coli/drug effects , Hot Temperature , Microbial Sensitivity Tests , Particle Size , Staphylococcus aureus/drug effects , Wound Healing/drug effectsABSTRACT
Copper nanoparticles were synthesized via precipitation technique using the pseudonatural cationic chitosan biopolymer as a stabilizing agent. The nanoparticles developed were successfully incorporated into the 1:1 ratio of blended chitosan: pluronic F127 polymer solution and made their nanocomposite hydrogels by solution casting method. The formed copper-based nanocomposite hydrogels were characterized by using Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy and transmission electron microscopy studies. The antimicrobial activity of the fabricated nanocomposite hydrogels was tested via an inhibition zone process against both E. coli (gram-negative) and S. aureus (gram-positive) bacteria. The results conveyed that the copper-embedded chitosan-pluronic\ F127 nanocomposite hydrogels can be used effectively for antimicrobial applications as well as for wound care applications.
Subject(s)
Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Chitosan/chemistry , Copper/chemistry , Nanocomposites/chemistry , Nanogels/chemistry , Chemical Phenomena , Chemistry Techniques, Synthetic , Escherichia coli/drug effects , Metal Nanoparticles/chemistry , Microbial Sensitivity Tests , Nanocomposites/ultrastructure , Spectroscopy, Fourier Transform Infrared , Thermogravimetry , X-Ray DiffractionABSTRACT
The objective of this study was to investigate the reduction and partial substitution effects of sodium chloride (NaCl) by potassium chloride (KCl) and calcium chloride (CaCl2) on lipolysis and lipid oxidation in salted meat aiming at reducing sodium content. To evaluate the effect of different salts on lipid oxidation thiobarbituric acid-reactive substances (TBARs) assay was performed along 180 days. Furthermore, ESI-MS/MS and GC analysis were conducted to detect and identify oxidized lipids, volatile compounds and free fatty acids profiles during the meat processing time. Lipid profiles from different salted meat demonstrated that CaCl2 salt have inducted more lipid oxidation when compared to the combination of NaCl and KCl salts, highlighting the implication of CaCl2 on increased lipolysis reactions. Moreover, the obtained results from both the analyses suggest that a combination of NaCl and KCl salts can be a good alternative for reducing the sodium content without compromising the quality of the salted meat.
ABSTRACT
Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field. This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs). Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid. TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7⯱â¯2â¯nm size. Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria. The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials. The results suggested that the synthesized materials can be used in wound dressing applications.
Subject(s)
Chitosan/chemistry , Copper/chemistry , Copper/pharmacology , Drug Resistance, Bacterial/drug effects , Metal Nanoparticles/chemistry , Nanocomposites/chemistry , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacillus/drug effects , Capsules , Escherichia coli/drug effects , Surface Properties , TemperatureABSTRACT
Alkaloids are interesting groups of natural products with important biological properties, but naturally available alkaloids are insufficient for biological studies. Therefore, the demand for higher amounts of alkaloids made research community to synthesize alkaloids by innovative techniques. The importance of asymmetric reactions for scientific community to obtain enantiomerically pure compounds with good yield and diastereomeric excess (de) or enantiomeric excess (ee) by different strategies of asymmetric induction is emphasized in this review. In addition, importance have been given to discuss on biologically important alkaloids, their skeleton synthesis, intermediates and total synthesis with different strategies mainly based on chiral auxiliaries, chiral reagents and chiral metal ligand based catalysts.
Subject(s)
Alkaloids/pharmacology , Biological Products/pharmacology , Alkaloids/chemistry , Animals , Biological Products/chemistry , Catalysis , Humans , Molecular Structure , StereoisomerismABSTRACT
Metabolic syndrome is estimated to affect more than one in five adults, and its prevalence is growing in the adult and pediatric populations. The most widely recognized metabolic risk factors are atherogenic dyslipidemia, elevated blood pressure, and elevated plasma glucose. Individuals with these characteristics commonly manifest a prothrombotic state and a proinflammatory state as well. Peroxisome proliferator-activated receptors (PPARs) may serve as potential therapeutic targets for treating the metabolic syndrome and its related risk factors. The PPARs are transcriptional factors belonging to the ligand-activated nuclear receptor superfamily. So far, three isoforms of PPARs have been identified, namely, PPAR- α, PPAR-ß/δ, and PPAR-γ. Various endogenous and exogenous ligands of PPARs have been identified. PPAR- α and PPAR- γ are mainly involved in regulating lipid metabolism, insulin sensitivity, and glucose homeostasis, and their agonists are used in the treatment of hyperlipidemia and T2DM. Whereas PPAR- ß / δ function is to regulate lipid metabolism, glucose homeostasis, anti-inflammation, and fatty acid oxidation and its agonists are used in the treatment of metabolic syndrome and cardiovascular diseases. This review mainly focuses on the biological role of PPARs in gene regulation and metabolic diseases, with particular focus on the therapeutic potential of PPAR modulators in the treatment of thrombosis.
