ABSTRACT
Cancer is a disease resulting from the disruption of cell cycle regulation, leading to the abnormal and unchecked proliferation of cells. Medicinal plants are rich in various bioactive phytochemicals or nutritional compounds. The aim is to determine the cytotoxic and genotoxic effects of ethanolic extracts of Macaranga peltata leaves on human oral cancer cell lines. The study setting was centre for Research on Molecular and Applied Sciences, Azeezia College of Dental Sciences and Research. The study design is a Comparative In Vitro study. Shade dried leaves of Macaranga peltata were subjected to Soxhlet extraction, and ethanolic extract was prepared. In vitro cytotoxic effects on human oral cancer cell lines were evaluated by (3-(4,5-dimethyl thiazole-2yl)-2,5-diphenyl tetrazolium bromide) MTT assay, and genotoxic effect was evaluated by comet assay. Untreated cell lines were used as control, and 5-fluorouracil was used as positive control. All experiments were performed in triplicates, and results were represented as Mean+/- SE. One-way ANOVA and Dunnet test were performed to analyze data. ***P < 0.001 compared with the control group. The ethanolic extract of Macaranga peltata exhibited cytotoxicity against oral cancer cells (LC50: 40.193089 µg/ml). There was a concentration-dependent increase in cell death, and at 100 µg/ml, the extract was most effective, causing 50% inhibition of viability. The comet assay showed significant genotoxic effects compared with 5-fluorouracil and untreated oral cancer cell lines. The ethanolic extract of Macaranga peltata leaves was subjected to MTT assay and comet using KB cell lines. The study concludes that the extract gave promising result for the anticancer activity on the KB cell lines producing cytotoxicity and genotoxicity.
ABSTRACT
BACKGROUND: Dentistry plays a crucial role in shaping the esthetics of a person and thus boosts the self-esteem of an individual. Whitening of the teeth is the most popular procedure where surface and deep stain removal whitens the teeth. Teeth whitening is achieved by professional application of chemicals and can also be self-administered by numerous products available. The most popular product is teeth whitening paste which is simple in application and easily available. Recently, teeth whitening tooth paste is commercially available where natural ingredients or their derivatives are used instead of chemicals for teeth whitening. AIMS: To investigate and compare the effect of commercially available chemical teeth whitening toothpaste and teeth whitening toothpaste containing ingredients of herbal origin on human enamel (at the Department of Oral and Maxillofacial Pathology, Royal Dental College, Kerala, India). MATERIALS AND METHODS: An in vitro study with a duration of 14 days was conducted. SETTINGS AND DESIGN: An in vitro study was conducted twenty samples were taken and assembled in to two groups of 10 each. Group A for teeth treated with conventional chemical toothpaste and group B for teeth treated with toothpaste containing ingredients of herbal origin. Premolars extracted for orthodontic purpose were checked for shade with Vita 3D shade guide and were observed under a stereomicroscope for surface morphology before the procedure. Each section was cleansed twice daily for 1 min with specified toothpaste and soft bristle toothbrush according to their groups and then cleansed with tap water and stored again in the same solution. After 14 days, the teeth were tested for the shade using vita 3D shade guide and surface morphology using stereomicroscope. STATISTICAL ANALYSIS USED: Student's unpaired t-test. RESULTS AND CONCLUSION: In this 14-day in vitro study, the chemical whitening toothpaste showed better whitening of teeth than toothpaste containing ingredient of herbal origin, whereas the surface irregularities was increased on the surface of the enamel with chemical whitening toothpaste when compared with toothpaste containing ingrtedients of herbal origin.
ABSTRACT
Extranodal non-Hodgkin lymphoma (NHL) is infrequent in the oral cavity and constitutes 3.5% of oral cancers, and less than 2.2% of maxillofacial lymphomas. Diffuse large B cell lymphoma (DLBCL) accounts for 40% of NHL and has a 5-year survival rate of less than 30%. Early detection of extranodal NHL by dental personnel is extremely important as a delay in diagnosis can result in the cancer being diagnosed at an advanced stage and a poor prognosis. A 60-year-old male presented with an uncharacteristic asymptomatic rapidly enlarging swelling of the anterior maxilla, which on histopathological and immunohistochemical analysis was diagnosed as DLBCL. Imaging studies showed bone invasion and lymph node metastasis with poor prognosis. The patient received radiotherapy and chemotherapy but died within 3 months of diagnosis. A literature search revealed one another case with anterior maxilla occurrence, as the few oral DLBCL so far reported have appeared on the posterior palate or other intraoral sites.
