The culture of lymphoma cells in diffusion chambers.

The leukemia blast cells from peripheral blood of 5 patients with acute leukemia were cultured in diffusion chambers implanted into peritoneal cavity of mice. In two patients with AML an increase of the blast counts was observed, whereas in 3 other cases the leukemic leukocytes number decreased more or less rapidly. The number of CLL lymphocytes cultured in diffusion chambers decreased more slowly in comparison with the control lymphocytes. A small increase in the number of leukemic and normal lymphocytes during culture was noted. The CLL lymphocytes with no acid phosphatase content during the culture in diffusion chambers demonstrated an increase of enzyme activity.

[Clinical usefulness of determination of the sensitivity of myeloblastic leukemia cells to antiproliferative drugs in cell culture in vivo]. / Przydatnosc kliniczna oznaczen wrazliwosci na leki antyproliferacyjne komórek ostrych bialaczek szpikowych w hodowli pllnnej in vivo.

In 15 patients with acute myeloblastic leukaemia and 4 with acute myelomonocytic leukaemia the sensitivity of blast cells was determined to cytosine arabinoside and and rubidomycin in cultures in diffusion chambers implanted into irradiated mice. The degree of sensitivity of leukaemic cells to antiproliferative drugs was determined after incubating the samples with cytosar and cerubidine on the basis of blood cell count changes on the 1st, 3rd and 6th days of the culture as compared with control determinations. The results of culture tests were confronted with the clinical condition of the patients. It was found that in cultures with the cells of the patients, in whom remission was achieved the number of myeloblasts decreased after incubation with cytosar and cerubidine from the 1st or 3rd day on. In patients refractory to treatment in most determinations the number of leukaemic cells incubated in cultures with the drugs remained at the level of the values obtained in control samples or after an initial slight fall this number increased again.

[Clonal growth of granulocytic progenitor cells in diffusion chambers in vivo]. / Wzrost klonalny komórek macierzystych ukierunkowanych szeregu granulocytarnego in vivo w komorach dyfuzyjnych.

Investigations on colony forming ability of committed granulocytic progenitors from bone marrow were carried out. In all, 85 cultures were performed. The bone marrow was obtained from 12 subjects without evident haematological abnormalities. Cultures were carried on for 9 days in 0.2% agar medium in diffusion chambers implanted into the peritoneal cavity of irradiated mice. It was found that committed granulocytic stem cells produced on the average 132 colonies (70--240), which were divided into four types according to their size and morphology. All types of colonic consisted of granulocytes, including polymorphonuclears. In cultures also were observed aggregations of cells containing monocytes.

[Lymphocyte acid phosphatase activity in lymphocytic leukemia studied in tissue culture with the use of diffusion chambers]. / Aktywnosc fosfatazy kwasnej limfocytów przewleklej bialaczki limfatycznej w hodowli z zastosowaniem komór dyfuzyjnych.

The reported investigations showed that lymphocytes in chronic lymphocytic leukaemia with low acid phosphatase activity had a rise of this enzyme level during culture in diffusion chamber. This rise is caused by increased number of lymphocytes with activity grades 1 degree and 2 degrees, and from the 6th day of culture also the number of lymphocytes with 3 degrees and 4 degrees activity score rose as well, while at the same time the proportion of cells without acid phosphatase activity fell. In the lymphocytes of healthy subjects a rise in acid phosphatase activity was observed as well under the same culture conditions.

[Phytohemagglutinin-stimulated lymphocyte proliferation in a diffusion chamber in vivo]. / Proliferacja limfocytów pod wplywem fitohemaglutyniny w warunkach hodowli in vivo z zastosowaniem komór dyfuzyjnych.

Lymphocyte proliferation stimulated with phytohaemagglutinin (PHA) was studied in vivo. For control aims experiments were done also lymphocytes without PHA. In the investigations the technique of diffusion chamber was used. Chambers with lymphocytes (1 X 10(6) cells in a chamber) were implanted into the peritoneal cavity of mice. On the 1st, 3rd, 6th, 10th, 13th, 17th and 29th days of culture the number of cells and the proportions of blast cells and small lymphocytes were determined. It was demonstrated that peripheral blood lymphocytes exposed to PHA proliferated in vivo. This was expressed initially as a rise in the blood cell count and this rise decreased more slowly than the number of cells in non-stimulated cultures.