Subject(s)
Genetic Predisposition to Disease/genetics , Hypersensitivity, Immediate , Immune System/immunology , Allergens/immunology , Animals , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Antibodies, Anti-Idiotypic/immunology , Diagnosis, Differential , Disease Progression , Glucocorticoids/therapeutic use , Humans , Hypersensitivity, Immediate/diagnosis , Hypersensitivity, Immediate/drug therapy , Hypersensitivity, Immediate/etiology , Immunoglobulins/immunology , Severity of Illness IndexABSTRACT
The authors studied luminol-dependent chemiluminescence of rabbit peripheral blood leukocytes during the development of Arthus' phenomenon. Samples with a specific allergen were previously incubated. It was established that in this type of allergic reactions the intensity of leukocyte chemiluminescence was determined by the concentration of the allergen with which they were incubated. Small doses of the allergen stimulate and large doses inhibit it. The inhibition is linked with the direct effect of the allergen on the cells and is not attended with diminution of their viability.
Subject(s)
Arthus Reaction/blood , Luminol/pharmacology , Neutrophils/drug effects , Allergens/immunology , Animals , Arthus Reaction/immunology , Epitopes , Luminescent Measurements , Neutrophils/chemistry , Neutrophils/immunology , RabbitsSubject(s)
Autoimmune Diseases/drug therapy , Glucocorticoids/therapeutic use , Adult , Animals , Autoimmune Diseases/immunology , Cells, Cultured , Dogs , Drug Resistance , Drug Therapy, Combination , Glucocorticoids/administration & dosage , Glucocorticoids/adverse effects , Guinea Pigs , Humans , Immunosuppressive Agents/administration & dosage , Lymphocyte Activation , Lymphocytes/drug effects , Lymphocytes/immunology , Rats , Substance-Related DisordersABSTRACT
IgE- and IgG-reagins were investigated in the sera of 120 patients with atopic bronchial asthma. Two clinicopathogenetic variants of disease were defined with relation to the genetic variants of disease were defined with relation to the main immunological mechanism: IgE-dependent (in 78 patients) and IgE-IgG-dependent (in 42 patients). Variations in a clinical picture, the results of specific allergological examination, the level of total serum IgE in different variants of the atopic form of bronchial asthma were established. Specific immunotherapy produced a good effect in the IgE-dependent variant. In the patients with the IgE-IgG-dependent variant specific immunotherapy with a house dust allergen proved to be ineffective in most cases.
Subject(s)
Antibodies/analysis , Asthma/diagnosis , Hypersensitivity, Immediate/diagnosis , Immunoglobulin E/analysis , Immunoglobulin G/analysis , Reagins/analysis , Adolescent , Adult , Aged , Asthma/etiology , Asthma/immunology , Diagnosis, Differential , Humans , Hypersensitivity, Immediate/etiology , Hypersensitivity, Immediate/immunology , Middle Aged , Skin TestsSubject(s)
Glucocorticoids/pharmacology , Lymphocytes/drug effects , Lymphoid Tissue/cytology , Animals , Dogs , Drug Resistance , Guinea Pigs , Humans , In Vitro Techniques , Male , Mice , RatsSubject(s)
Arthritis, Experimental/metabolism , Arthritis/metabolism , Hydroxycorticosteroids/metabolism , Liver/metabolism , Lymphocytes/metabolism , Progesterone/therapeutic use , 11-Hydroxycorticosteroids/metabolism , Animals , Arthritis, Experimental/drug therapy , Corticosterone/metabolism , Hydrocortisone/metabolism , Liver/pathology , Progesterone Congeners/therapeutic use , RatsSubject(s)
Arthritis, Experimental/immunology , Arthritis/immunology , Corticosterone/antagonists & inhibitors , Lymph Nodes/immunology , Lymphocytes/immunology , Lymphoid Tissue/immunology , Peyer's Patches/immunology , Animals , Cell Survival/drug effects , Corticosterone/metabolism , Kinetics , Leukocyte Count , Lymphocytes/drug effects , Male , RatsABSTRACT
Lymphocytes from the cervical lymph nodes of guinea pigs were incubated in medium 199 in the presence of cortizol in a concentration of 20 and 100 microng% for 24 hours. A determination was made of the lymphocyte survival and cortizol metabolism by them, and also (cytophotometrically) the nucleic acid content. There proved to be a marked reduction of cortizol metabolism by the lymphocytes from the 6th day after the administration of the encephalytogenic mixture and a marked increase of the cortizol-resistant population of lymphocytes in the guinea pigs with encephalomyelitis, and on the 17th--30th day after the administration of a complete stimulant. In a concentration of 100 microng% cortizol reduced the nucleic acid content in the lymphocytes of the intact animals, but had no influence upon the lymphocytes of both experimental groups of guinea pigs at the period of a marked increase of the cortizol-resistant population. Progesteron decreased the lympholytic action of cortizol and the metabolism of this hormone by the lymphocytes of intact guinea pigs. The capacity of progesteron to decrease the lympholytic action of cortizol was weakened in guinea pigs with encephalomyelitis and in the animals given the stimulant.
Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Hydrocortisone/pharmacology , Lymph Nodes/immunology , Lymphocytes/immunology , Animals , Guinea Pigs , Hydrocortisone/metabolism , In Vitro Techniques , Lymphocytes/drug effects , Lymphocytes/metabolism , Male , Progesterone/pharmacology , RNA/metabolismABSTRACT
Lymphocytes from the cervical lymph nodes of guinea pigs were incubated in medium 199 in the presence of cortizol in a concentration of 20 and 100 microng% for 24 hours. A determination was made of the lymphocyte survival and cortizol metabolism by them, and also (cytophotometrically) the nucleic acid content. In the intact and the sesitized guinea pigs progesterone in a concentration of 10-5 inhibited both the lytic action of coritizol and its metabolism There was observed a marked reduction of cortizol meta bolism by the lymphocytes from the 6th day after the sensitization, which was restored only by the 90th day. The cortizol-resistant lymphocyte population increased on the 17th--30th day. In a concentration of 100 microng% cortizol reduced the nucleic acid content in the lymphocytes of both the intact guinea pigs and sensitized ones on the 17th--30th day, when the cortizol-resistant population increased.
Subject(s)
Hydrocortisone/pharmacology , Hypersensitivity/immunology , Lymphocytes/immunology , Animals , Blood Proteins/immunology , Drug Interactions , Guinea Pigs , Hydrocortisone/metabolism , Lymph Nodes/immunology , Lymphocytes/drug effects , Lymphocytes/metabolism , Male , Progesterone/pharmacology , Time FactorsABSTRACT
The intensity of cortisol metabolism was studied under conditions of perfusion of the liver in situ by solutions containing cortisol in different concentrations; in this case metabolism coursed chiefly in the direction of cortisone formation. With the increase of cortisol concentration in the inflowing perusate there was also a rise in the intensity of its metabolism in the hepatic tissue. In anaphylactic alteration of hepatic tissue the intensity of cortisol transformation into cortisone decreased, this pointing to disturbances of cortisol oxidation. Experimental allergic encephalomyelitis is also acccompanied by a reduction in the intensity of cortisol metabolism.