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1.
Water Sci Technol ; 81(4): 744-752, 2020 Feb.
Article in English | MEDLINE | ID: mdl-32460277

ABSTRACT

Biofouling is unwanted accumulation of microbial population on the membrane surface which limits the use of membrane bioreactor (MBR) in the market. Disruption of the biofilm formation by Quorum Quenching (QQ) by using cell entrapping beads (CEBs) is an approach with great potential to control membrane biofouling as the beads used provide not only mitigating effect on biofilm formation, by interfering Quorum Sensing, but also physical forces to detach the biofilm from the membrane surface. This research aimed to develop QQ-CEB with locally available chemicals in Pakistan and its application to evaluate the QQ effect together with physical and chemical cleaning. Various CEBs were made of different mixtures of sodium alginate and polyvinyl alcohol (PVA) and their quality was tested considering physical and biological aspects. Rhodococcus sp. BH4 and Pseudomonas putida were entrapped in the CEBs and then introduced in MBR as one of biofouling control methods along with standard backwash and chemical backwash. The CEBs made of specific concentration of PVA were proven to be more durable and helpful in mitigating biofouling as compared to that of sodium alginate. An MBR operated with PVA-alginate QQ CEBs together with chemical backwash showed the best performance without deterioration of effluent quality.


Subject(s)
Biofouling , Quorum Sensing , Bioreactors , Membranes , Membranes, Artificial
2.
J Helminthol ; 93(5): 533-538, 2019 Sep.
Article in English | MEDLINE | ID: mdl-30149818

ABSTRACT

In this study, we evaluated the in vivo comparative efficacy of ivermectin and Nigella sativa extract against helminths in Aseel chickens, and the effects of helminths on blood parameters before and after treatment in Aseel chickens. Forty naturally infected adult Aseel chickens were randomly divided into four groups (n = 10 each): group A (ivermectin at 300 µg/kg); group B (N. sativa extract at 200 mg/kg); group C (ivermectin at 300 µg/kg + N. sativa extract at 200 mg/kg); group D was kept as a positive control to monitor time-related changes. On day 28 post treatment, the mean percentages of faecal egg-count reduction (FECR %) in groups A, B and C were recorded as 93.58, 88.09 and 100.00%, respectively. Further data analysis showed significantly higher efficacy in group C (100 ± 0.00%) than in groups A and B (P < 0.001). Highly significant (P < 0.001) improvements in mean percentage values of packed cell volume (PCV %) were recorded in groups A and C on days 14 and 28 post treatment. Meanwhile, the improvements in mean values of haemoglobin (Hb) concentration in groups A, B and C were highly significant (P < 0.001) when compared to that of group D on day 28 post treatment. The synergistic combination of ivermectin and N. sativa extract possessed greater efficacy than either ivermectin or N. sativa extract used alone. Furthermore, both PCV % and Hb concentration values gradually increased in the treated groups compared to the control group, in which PCV % and Hb concentration gradually decreased throughout the trial.


Subject(s)
Helminthiasis, Animal/drug therapy , Ivermectin/therapeutic use , Nigella sativa/chemistry , Plant Extracts/therapeutic use , Animals , Chickens/parasitology , Drug Synergism , Feces/parasitology , Helminths/drug effects , Parasite Egg Count , Random Allocation
3.
Anaesthesia ; 73(5): 579-586, 2018 May.
Article in English | MEDLINE | ID: mdl-29349776

ABSTRACT

The Difficult Airway Society 2015 guidelines recommend and describe in detail a surgical cricothyroidotomy technique for the can't intubate, can't oxygenate (CICO) scenario, but this can be technically challenging for anaesthetists with no surgical training. Following a structured training session, 104 anaesthetists took part individually in a simulated can't intubate, can't oxygenate event using simulation and airway models to evaluate how well they could perform these front-of-neck access techniques. Main outcomes measures were: ability to correctly perform the technical steps; procedural time; and success rate. Outcomes were compared between palpable and impalpable cricothyroid membrane scenarios. Anaesthetists' technical abilities were good, as assessed by a video analysis checklist score. Mean (SD) procedural time was 44 (16) s and 65 (17) s for the palpable and impalpable cricothyroid membrane models, respectively (p ≤ 0.001). First-pass tracheal tube placement was obtained in 103 out of the 104 palpable cricothyroidotomies and in 101 out of the 104 impalpable cricothyroidotomies (p = 0.31). We conclude that anaesthetists can be trained to perform surgical front-of-neck access to an acceptable level of competence and speed when assessed using a simulator.


Subject(s)
Emergency Medical Services , Laryngeal Muscles/surgery , Neck/surgery , Palpation , Adult , Airway Management , Anesthesiology/education , Clinical Competence , Female , Humans , Internship and Residency , Intubation, Intratracheal , Laryngeal Muscles/anatomy & histology , Male , Manikins , Neck/anatomy & histology , Obesity/complications , Thyroidectomy
4.
Environ Monit Assess ; 141(1-3): 323-8, 2008 Jun.
Article in English | MEDLINE | ID: mdl-17849229

ABSTRACT

In this investigation properties of organic semiconductor copper phthalocyanine (CuPc) capacitive humidity and illumination sensors were studied. Organic thin film was deposited by vacuum evaporation on a glass substrate with silver surface-type electrodes to form the Ag/CuPc/Ag sensor. The capacitance of the samples was evaluated at room temperature in the relative humidity range of 35-92%. It was observed that capacitance of the Ag/CuPc/Ag sensor increases with increase in humidity. The ratio of the relative capacitance to relative humidity was about 200. It is assumed that in general the capacitive response of the sensor is associated with polarization due to absorption of water molecules and transfer of charges (electrons and holes). It was observed that under filament lamp illumination of up to 1,000 lx the capacitance of the Ag/CuPc/Ag photo capacitive detectors increased continuously by 20% as compared to dark condition. It is assumed that photo capacitive response of the sensor is associated with polarization due to transfer of photo-generated electrons and holes. An equivalent circuit of the Ag/CuPc/Ag capacitive humidity and illumination sensor was developed. Humidity and illumination dependent capacitance properties of this sensor make it attractive for use in humidity and illumination multi-meters. The sensor may be used in instruments for environmental monitoring of humidity and illumination.


Subject(s)
Environmental Monitoring/instrumentation , Humidity , Indoles/chemistry , Organometallic Compounds/chemistry , Semiconductors
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