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1.
Zhonghua Jie He He Hu Xi Za Zhi ; 47(3): 249-251, 2024 Mar 12.
Article in Chinese | MEDLINE | ID: mdl-38448177

ABSTRACT

Nasopharyngeal tuberculosis refers to the tuberculosis in the nasopharynx, which is mainly treated with systemic chemotherapy with anti-tuberculosis drugs. Here, we reported a case of nasopharyngeal tuberculosis treated by cryosurgery combined with local spraying of isoniazid on the basis of systemic chemotherapy with anti-tuberculosis drugs. By reviewing the case data and relevant literature, we understood the clinical manifestations, diagnosis and differential diagnosis of the disease, improved everyone's understanding of the disease, and proposed a new method of cryosurgery combined with local spraying of isoniazid for the treatment of nasopharyngeal tuberculosis for clinical discussion.


Subject(s)
Cryotherapy , Isoniazid , Humans , Isoniazid/therapeutic use , Antitubercular Agents/therapeutic use , Diagnosis, Differential
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(10): 1022-1026, 2019 Oct 06.
Article in Chinese | MEDLINE | ID: mdl-31607049

ABSTRACT

Objective: To analyze the quadrivalent influenza vaccine intention of 718 health care workers (HCWs) in the Pearl River Delta region from 2015 to 2017. Method: In May 2018, 718 HCWs from the department related to the diagnosis and treatment of influenza in 17 hospitals (6 tertiary hospitals, 5 secondary hospitals and 6 primary hospitals) from Guangzhou, Jiangmen, Zhuhai and Dongguan were selected by using stratified sampling method. Questionnaire survey and face-to-face interview were used to collect the information of influenza vaccination, the intention of the quadrivalent influenza vaccine, the acceptance of free and required vaccination policies, and recommendations for increasing influenza vaccination intentions from 2015 to 2017. The multivariate logistic regression was used to analyze factors associated with the vaccination intention. Results: A total of 718 HCWs were surveyed and 147 of them were interviewed face to face. Among them, the vaccination rate of primary hospitals [17.39%(40/230)] was higher than that of other hospitals (χ(2)=15.80, P<0.05). If the vaccine could be free, 84.82% (609/718) of HCWs would like to be vaccinated. The multivariate logistic regression showed that the factors, HCWs who were aged ≥50 years (OR=3.44, 95%CI:1.43-8.28), worked in department of prevention and health care (OR=2.35, 95%CI:1.16-4.75), learned about the quadrivalent influenza vaccine (OR=2.94, 95%CI:2.08-4.18), knowed that HCWs are priority (OR=2.33, 95%CI:1.56-3.48), and had a history of trivalent influenza vaccination from 2015 to 2017 (OR=4.70, 95%CI:3.08-7.15), were associated with the vaccination intention. Conclusion: HCWs in the Pearl River Delta region had weak inclination of getting quadrivalent influenza vaccine. HCWs who were age (≥50 years old), worked in department of prevention and health care, learned about the quadrivalent influenza vaccine, knowed that HCWs are priority, and had a history of trivalent influenza vaccination from 2015 to 2017 were factors positively associated with the vaccination intention.


Subject(s)
Health Personnel , Influenza Vaccines , Influenza, Human , Attitude of Health Personnel , China/epidemiology , Cross-Sectional Studies , Humans , Intention , Middle Aged , Patient Acceptance of Health Care , Surveys and Questionnaires , Vaccination
4.
J Int Med Res ; 39(5): 2039-44, 2011.
Article in English | MEDLINE | ID: mdl-22118010

ABSTRACT

Repetitive transcranial magnetic stimulation (rTMS) has been used as an investigative and therapeutic tool in neuropsychiatry and is advocated as a therapy for the treatment of psychiatric disorders, particularly adult depression. The therapeutic efficacy of current antidepressants applied to children or adolescents is unsatisfactory; thus innovative treatments such as rTMS are in demand. Large-scale clinical studies are required to determine the safety of rTMS for this age group. The present case report describes a 15-year-old female with depression who had seizure and hypomania during the first session of rTMS concomitant to sertraline 100 mg/day orally. The patient continued on this regimen of sertraline therapy but rTMS was not repeated. Subsequent electroencephalogram (EEG) examination detected no abnormalities and no long-term complications were observed. We suggest that rTMS should be used cautiously to treat adolescents with depressive disorders, particularly when used concomitant to antidepressant treatment.


Subject(s)
Depressive Disorder/diagnosis , Seizures/etiology , Transcranial Magnetic Stimulation/adverse effects , Adolescent , Antidepressive Agents/therapeutic use , Combined Modality Therapy , Depressive Disorder/therapy , Female , Humans , Sertraline/therapeutic use
5.
J Cancer Res Clin Oncol ; 128(11): 617-20, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12458342

ABSTRACT

PURPOSE: The roles of terminal sialyl and fucosyl residues in cell surface glycans in the metastatic potential of H7721 cells, a human hepatocarcinoma cell line, were studied. METHODS: Neuraminidase and alpha-L-fucosidase were used to remove the sialyl and fucosyl residues, respectively. Cell adhesion to fibronectin (Fn), laminin (Ln), and human umbilical vein epithelial cell (HUVEC), as well as chemotactic cell migration and invasion, were selected as the parameters of metastatic potential ex vivo. RESULTS: Sialyl residue is not essential for cell adhesion to Fn, but is important in cell adhesion to Ln and invasion, and is crucial in cell adhesion to HUVEC and migration. In contrast, fucosyl residue contributes more than sialyl residue to cell adhesion to Fn and Ln, but less to adhesion to HUVEC, and is not essential in chemotactic cell migration and invasion. Cell adhesion to HUVEC, migration, and invasion were inhibited by the monoclonal antibody of sialyl Lewis X, but not by the antibody of non-sialyl Lewis X. CONCLUSION: Terminal sialyl residues on cell surface glycans are more important than fucosyl residues in mediating cell adhesion to HUVEC and cell migration/invasion, but the reverse is true in cell adhesion to Fn and Ln.


Subject(s)
Antigens, Tumor-Associated, Carbohydrate/physiology , Carcinoma, Hepatocellular/metabolism , Fucose/physiology , Liver Neoplasms/metabolism , Sialic Acids/physiology , Carcinoma, Hepatocellular/pathology , Cell Adhesion , Cell Movement , Chemotaxis , Epithelial Cells/metabolism , Fibronectins/metabolism , Humans , Laminin/metabolism , Lewis Blood Group Antigens , Liver Neoplasms/pathology , Membrane Glycoproteins/metabolism , Neoplasm Metastasis , Oligosaccharides/immunology , Sialyl Lewis X Antigen , Tumor Cells, Cultured , Umbilical Veins
6.
Eur J Biochem ; 268(12): 3501-12, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11422380

ABSTRACT

The pCMV4 plasmid containing the cancer-promoting gene, c-erbB2/neu, was cotransfected into the human hepatocarcinoma cell line 7721 with the pcDNA3 vector, which contains the 'neo' selectable marker. Several clones showing stable expression of c-erbB2/neu were established and characterized by determination of c-erbB2/neu mRNA and its encoded protein p185. Expression of Lewis antigens and alpha1,3-fucosyltransferases and the biological behavior of 7721 cells after c-erbB2/neu transfection were studied using mock cells transfected with the vectors pCMV4 and pcDNA3 as controls. SLe(x) expression on the surface of mock cells was high, whereas expression of SDLe(x), Lex and SLe(a) was absent or negligible. This is compatible with the abundant expression of alpha1,3-fucosyltransferase VII, very low expression of alphafucosyltransferase III/VI, and almost absent expression of alpha1,3-fucosyltransferase IV in the mock cells. After transfection of c-erbB2/neu, expression of SLe(x) and alpha1,3-fucosyltransferase VII were simultaneously elevated, but that of alphafucosyltransferase III/VI was not altered. The expression of both SLe(x) and alpha1,3-fucosyltransferase VII correlated positively with the expression of c-erbB2/neu in different clones, being highest in clone 13, medium in clone 6, and lowest in clone 7. In addition, the adhesion of 7721 cells to human umbilical vein endothelial cells (HUVECs) or P-selectin, as well as cell migration and invasion, were increased in c-erbB2/neu-transfected cells. These increases also correlated positively with the expression intensities of c-erbB2/neu, SLe(x) and alpha1,3-fucosyltransferase VII in the different clones, whereas cell adhesion to fibronectin correlated negatively with these variables. mAbs to SLe(x) (KM93) and SDLe(x) (FH6) significantly and slightly, respectively, abolished cell adhesion to HUVECs or P-selectin and cell migration and invasion. mAbs to SDLe(x) and SLe(a) did not suppress cell adhesion to HUVECs nor inhibit cell migration and invasion. Transfection of alpha1,3-fucosyltransferase VII cDNA into 7721 cells showed similar results to transfection of c-erbB2/neu, and the increased adhesion to HUVECs, cell migration, and invasion were also inhibited significantly by KM93 and slightly by FH6. These results indicate that expression of alpha1,3-fucosyltransferase VII and its specific product, SLe(x), and their capacity for cell adhesion, migration and invasion are closely related. Therefore, the c-erbB2/neu gene is proposed to be a metastasis-promoting gene, and its effects are at least partially mediated by the increased expression of alpha1,3-fucosyltransferase VII and SLe(x).


Subject(s)
Carcinoma, Hepatocellular/genetics , Fucosyltransferases/genetics , Genes, erbB-2 , Liver Neoplasms/genetics , Neoplasm Metastasis/genetics , Oligosaccharides/genetics , Transfection , Up-Regulation/genetics , Antibodies/immunology , Carcinoma, Hepatocellular/enzymology , Carcinoma, Hepatocellular/immunology , Carcinoma, Hepatocellular/pathology , Cell Adhesion/genetics , Cells, Cultured , DNA, Complementary , Endothelium, Vascular/cytology , Endothelium, Vascular/metabolism , Fibronectins/metabolism , Humans , Liver Neoplasms/enzymology , Liver Neoplasms/immunology , Liver Neoplasms/pathology , Oligosaccharides/immunology , P-Selectin/metabolism , Phenotype , RNA, Messenger/genetics , Sialyl Lewis X Antigen , Tumor Cells, Cultured
7.
Br J Cancer ; 84(11): 1556-63, 2001 Jun 01.
Article in English | MEDLINE | ID: mdl-11384108

ABSTRACT

The expressions of Lewis (Le) antigens, alpha-1,3/1,4 fucosyltransferases (alpha-1,3/1,4 FuTs), and metastatic potential after the treatment of 2 differentiation inducers, all- trans retinoic acid (ATRA), 8-bromo-cyclic 3',5'adenosine monophosphate (8-Br-cAMP); and 2 proliferation inducers, epidermal growth factor (EGF) and phobol-12-myristate-13-acetate (PMA), on 7721 human hepatocarcinoma cell line were studied. Cell adhesion to human umbilical vein endothelial cells (HUVEC), cell migration through transwell and invasion through matrigel were selected as the indexes of metastatic potential-related phenotypes. Using fluorescence-labelled antibodies and flow-cytometric analysis, it was found that 7721 cells mainly expressed sialyl Lewis X (SLe(x)) and a less amount of sialyl dimeric Lewis X (SDLe(x)) antigens on the cell surface. Their expressions were down-regulated by ATRA, and up-regulated by EGF. SLe(x)antigen was also decreased and increased by the treatment of 8-Br-cAMP and PMA respectively. With Northern blot to detect the mRNAs of alpha-1,3/1,4 FuTs, the main enzymatic basis for the change in SLe(x)expression was found to be the alteration of the expression of alpha-1,3 FuT-VII. It was evidenced by the observations that alpha-1,3 FuT-VII was the main alpha-1,3/1,4 FuT in 7721 cells, while alpha-1,3/1,4 FuT-III and alpha-1,3 FuT-VI were expressed rather low. The changes in the expressions of SLe(x)antigen and alpha-1,3 FuT-VII resulted in the altered cell adhesion to tumour necrosis factor-alpha stimulated HUVEC, since only the monoclonal antibody of the SLe(x), but not other monoclonal antibodies blocked the adhesion of 7721 cells to HUVEC. The migration and invasion of 7721 cells were also reduced by the treatment of ATRA or 8-Br-cAMP, and elevated by EGF or PMA. The above findings indicate that the metastatic potential of 7721 cells is suppressed by differentiation-inducers and promoted by proliferation-inducers.


Subject(s)
Carcinoma, Hepatocellular/pathology , Cell Differentiation , Cell Division , Fucosyltransferases/biosynthesis , Lewis Blood Group Antigens/biosynthesis , Lewis Blood Group Antigens/immunology , Lewis X Antigen/biosynthesis , Liver Neoplasms/pathology , Neoplasm Metastasis , 8-Bromo Cyclic Adenosine Monophosphate/pharmacology , Antineoplastic Agents/pharmacology , Carcinoma, Hepatocellular/immunology , Cell Adhesion , Cell Movement , Epidermal Growth Factor/pharmacology , Fucosyltransferases/metabolism , Humans , Liver Neoplasms/immunology , Phenotype , Tetradecanoylphorbol Acetate/pharmacology , Tretinoin/pharmacology , Tumor Cells, Cultured
8.
J Cancer Res Clin Oncol ; 126(2): 85-90, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10664247

ABSTRACT

The effects of all-trans retinoic acid (ATRA) and epidermal growth factor (EGF) on the expression of nm23-H1, a metastasis suppressor gene, were studied in a human 7721 hepatocarcinoma cell line. It was discovered that the expression of nm23-H1 mRNA was up-regulated by ATRA. This was compatible with the observation that the metastasis-associated phenotypes, such as chemotaxic cell migration and invasion, were both reduced in the ATRA-treated and nm23-H1-cDNA-transferred 7721 cells. However, ability of cells to adhere to fibronectin and laminin was not altered identically in the ATRA-treated and nm23-H1-cDNA-transfected 7721 cells. In contrast, the expression of nm23-H1 mRNA in 7721 cells was down-regulated both by the treatment with EGF and by the transfection of c-erbB-2/neu cDNA, which codes a protein homologous to the EGF receptor. EGF is a compound with biological effects opposite to those of ATRA, and c-erbB-2/neu is known to be a metastasis-promoting gene. These results reveal that the metastasis-preventing effect of ATRA may partly result from the up-regulation of nm23-H1, and the metastasis-promoting effects of EGF and c-erbB-2/neu were probably mediated in part by the down-regulation of nm23-H1.


Subject(s)
Antineoplastic Agents/pharmacology , Carcinoma, Hepatocellular/pathology , Epidermal Growth Factor/pharmacology , Gene Expression Regulation, Neoplastic , Genes, Tumor Suppressor/genetics , Liver Neoplasms/pathology , Monomeric GTP-Binding Proteins/genetics , Nucleoside-Diphosphate Kinase , Transcription Factors/genetics , Tretinoin/pharmacology , Carcinoma, Hepatocellular/genetics , Humans , Liver Neoplasms/genetics , NM23 Nucleoside Diphosphate Kinases , Neoplasm Metastasis/physiopathology , Neoplasm Metastasis/prevention & control , Promoter Regions, Genetic , RNA, Messenger/biosynthesis , Tumor Cells, Cultured/drug effects
9.
Yao Xue Xue Bao ; 32(8): 612-6, 1997 Aug.
Article in Chinese | MEDLINE | ID: mdl-11596312

ABSTRACT

Microcrystalline cellulose triacetate (CTA) was prepared by heterogeneous acetylation of microcrystalline cellulose and used as liquid chromatographic chiral stationary phase and chiral thin layer plate to separate pharmaceutical enantiomers. The racemic Troegor's base, mathaqualone, chlormezanone and chloroquine were separated on the CTA chiral column and on the CTA chiral plate. 95% ethanol and the mixtures of 95% ethanol and water with different ratio and different pH values were used as mobile phase. The influences of eluent composition, pH and temperature on the chiral separation were discussed. The experimental results indicate that the CTA chiral plate was more sensitive to eluent composition and pH than chiral column possibly because the chiral adsorbent was not swollen on the plate.


Subject(s)
Cellulose/analogs & derivatives , Chromatography, High Pressure Liquid , Stereoisomerism
10.
Infect Immun ; 62(6): 2432-9, 1994 Jun.
Article in English | MEDLINE | ID: mdl-8188368

ABSTRACT

Porins from different neisserial strains and species have been shown to have differences in both primary amino acid sequence and biophysical characteristics as observed by functional assays. A closer examination of how the changes in the primary amino acid sequence of Neisseria porin molecules correlate with these observed biophysical changes has been impeded by the inability to easily manipulate the cloned porin genes by modern molecular techniques and then obtain enough of the expressed modified porin protein to purify and use in these biophysical functional assays. In this report, we describe a method by which the genes encoding three different porin proteins, lacking their neisserial promoter and signal sequences, were cloned into an expression plasmid and transformed into Escherichia coli. Upon induction, large amounts of the porin proteins were produced. The expressed porin proteins were then manipulated to regenerate their native trimer structure and purified by standard protein chemistry. Sufficient purified recombinant porin protein was obtained for further antigenic as well as biophysical characterization. This sets the stage for the biophysical characterization of these neisserial porin proteins in more detail.


Subject(s)
Escherichia coli/genetics , Neisseria/chemistry , Porins/biosynthesis , Protein Folding , Recombinant Proteins/biosynthesis , Base Sequence , Cloning, Molecular , Enzyme-Linked Immunosorbent Assay , Molecular Sequence Data , Polymerase Chain Reaction , Porins/chemistry , Porins/genetics
11.
Sci China B ; 32(7): 816-21, 1989 Jul.
Article in English | MEDLINE | ID: mdl-2818818

ABSTRACT

Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouse livers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and high speed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured by radioimmunoassay. The basic method offers opportunities for further increases of yield and for the enrichment of SFA of other vertebrates.


Subject(s)
Scleroderma, Systemic/genetics , Spindle Apparatus , Transfection , Animals , Cell Nucleus/immunology , DNA Probes , Immunoglobulin G , Liver/ultrastructure , Mice
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