ABSTRACT
Objective: To investigate miR-18a-5p and ataxia telangiectasia muted (ATM) expression in esophageal squamous cell carcinoma (ESCC) and their correlations with clinicopathological features. Methods: The subjects of this study were 62 ESCC patients (research group, RG) and 57 healthy controls (control group, CG) presented to our hospital between July 2019 and April 2020. Peripheral blood (PB) miR-18a-5p and ATM levels in these participants were quantified via qRT-PCR, and the correlations of the two genes with ESCC patients' clinicopathological characteristics were investigated. In addition, a two-year follow-up was performed on ESCC patients to understand their survival, so as to further determine the prognostic utility of miR-18a-5p and ATM in ESCC. Factors influencing patient outcomes were identified by COX analysis. Results: PB miR-18a-5p expression was higher in RG compared with CG, while ATM was lower, suggesting an inverse connection between the two genes in ESCC (P < 0.001). miR-18a-5p and ATM levels were determined to be strongly linked to TNM stage, differentiation degree, and lymph node metastasis in ESCC patients (P < 0.001 and P = 0.007). The patients who succumbed to the disease exhibited higher miR-18a-5p and lower ATM than the survival (P < 0.05). ROC analysis suggested favorable evaluation effects of miR-18a-5p and ATM on the occurrence and prognostic death of ESCC (P < 0.001). Further, these two genes were identified by the COX analysis to be factors independently affecting the prognosis of ESCC. Conclusion: miR-18a-5p is highly expressed in ESCC, and ATM is underexpressed, both of which are closely linked to the pathological process of ESCC and have a good evaluation effect on the occurrence and prognosis of ESCC, which may become a breakthrough in future diagnosis and treatment of ESCC.
Subject(s)
Ataxia Telangiectasia , Esophageal Neoplasms , Esophageal Squamous Cell Carcinoma , MicroRNAs , Humans , Esophageal Squamous Cell Carcinoma/genetics , Esophageal Neoplasms/genetics , Ataxia Telangiectasia/genetics , MicroRNAs/genetics , Cell Line, Tumor , Gene Expression Regulation, Neoplastic , Cell Proliferation , Ataxia Telangiectasia Mutated Proteins/genetics , Ataxia Telangiectasia Mutated Proteins/metabolismABSTRACT
Aberrant expression of microRNA-497 (miR-497) is associated with tumor progression, but the molecular mechanisms in tumorigenesis remain largely unknown. Here, we report that miR-497 expression is downregulated in esophageal squamous cell carcinoma (ESCC) clinical samples. Consistently, upregulation of miR-497 inhibits ESCC cell malignant properties and tumor growth in vivo. Importantly, we uncovered that miR-497 upregulation suppressed ESCC cell growth and tumor growth by inhibiting Smurf2. Mechanistically, we showed that Smurf2 was a target of miR-497, and mediated YY1 expression to elevate HIF2α expression, thereby enhancing the malignancy of ESCC cells. Together, our study uncovered the role of the miR-497-mediated Smurf2/YY1/HIF2α axis in tumor growth and metastasis, which might provide potential therapeutic targets for human ESCC.
Subject(s)
Esophageal Neoplasms , Esophageal Squamous Cell Carcinoma , MicroRNAs , Humans , Esophageal Squamous Cell Carcinoma/genetics , Esophageal Squamous Cell Carcinoma/metabolism , Esophageal Neoplasms/genetics , Esophageal Neoplasms/metabolism , MicroRNAs/genetics , Cell Line, Tumor , Up-Regulation , Cell Proliferation , Gene Expression Regulation, Neoplastic , Cell Movement , Neoplasm Invasiveness/genetics , Ubiquitin-Protein Ligases/genetics , Ubiquitin-Protein Ligases/metabolism , YY1 Transcription Factor/genetics , YY1 Transcription Factor/metabolismABSTRACT
OBJECTIVE: To investigate the clinical value of molecular biology (xpert MTB/RIF) combined with a liquid mycobacterium culture tube (MGIT) in the clinical examination of Mycobacterium tuberculosis (MTB). METHODS: A total of 782 patients with suspected pulmonary tuberculosis who came to our hospital from February 2018 to February 2020 were selected as the research subjects. Sputum samples of all patients were taken in the morning, and BACTEC MGIT culture and xpert MTB/RIF, Lowenstein-Jenden (L-J) culture and acid fast staining microscopy were used respectively to detect the sputum samples. The positive samples were cultured, and the bacteria identification and liquid drug sensitivity test were carried out. We also analyzed the value of xpert MTB/RIF combined with MGIT culture method in the clinical testing of MTB. RESULTS: (1) Among the 782 suspected patients, 405 cases were diagnosed with pulmonary tuberculosis and 377 cases were non tuberculosis patients. The sensitivity of smear microscopy was 17.28% (70/405), the specificity was 98.94% (373/377), and the average time was 3.05 h. The sensitivity of L-J culture test was 20.49% (83/405), the specificity was 97.61% (368/377), and the average time was 28.58 h. The sensitivity of MGIT culture test was 38.02% (154/405), the specificity was 96.82% (365/377), and the average time was 11.23 h. The sensitivity of xpert MTB/RIF test was 36.54% (148/405), the specificity was 99.46% (375/377), and the average time was 2.03 h. The sensitivity of MTB/RIF + MGIT test was 42.47% (172/405), the specificity was 96.02% (362/377), and the average time was 11.29 h. The sensitivity of MTB/RIF combined with MGIT culture was significantly higher than that of smear microscopy and L-J culture (χ2=61.31, 45.33, P<0.001). (2) The average culture time of 13 smear negative xpert MTB/RIF negative specimens was 17.02 days. The average culture time of 82 smear negative xpert MTB/RIF positive samples was 14.12 days. The average culture time of 77 smear positive xpert MTB/RIF positive samples was 7.45 days. (3) The sensitivity of Xpert MTB/RIF test for rifampicin resistance is 100% (11/11), and the specificity is 91.14% (144/158). CONCLUSION: The method of molecular biology combined with MGIT has a high sensitivity and specificity for clinical diagnosis of MTB.
