ABSTRACT
Chemical constituents from the acetone extract of twigs of Manglietia hookeri were isolated and purified by various column chromatographic methods over silica gel and sephadex LH-20, and preparative TLC. The structures of these compounds were identified on the basis of physicochemical properties and spectral analysis, including NMR and MS spectra. Six eudesmane sesquiterpenes were obtained and their structures were identified as trans-eudesmane-4, 11-diol(1), ß-eudesmol(2), (-) -10-epi-5ß-hydroxy-ß-eudesmol (3), epi-carrisone (4), 6-hydroxy-eudesm-4(14) -ene(5) and gynurenol(6). All the compounds were isolated from this plant for the first time. Furthermore, the 13C-NMR data of compound 3 were reported for the first time.
Subject(s)
Drugs, Chinese Herbal/chemistry , Magnolia/chemistry , Sesquiterpenes, Eudesmane/chemistry , Drugs, Chinese Herbal/isolation & purification , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Stems/chemistry , Sesquiterpenes, Eudesmane/isolation & purification , Spectrometry, Mass, Electrospray IonizationABSTRACT
PURPOSE: The association between apolipoprotein E (ApoE) gene polymorphism and the risk of recurrent pregnancy loss (RPL) remains controversial. The present meta-analysis was performed to derive a precise estimate of the relationship. METHOD(S): We searched the PubMed, Embase, and Web of Science data- bases for studies related to the association between the ApoE genotype and the risk of RPL. We estimated the summary odds ratio (OR) with 95% confidence interval (CI) to assess the association. RESULT(S): Seven studies, including 2,090 RPL cases and 742 control samples, were identified. The results showed a significant association between ApoE E4 mutation and RPL risk (for E4 allele: OR =1.98, 95 %CI =1.14-3.43, P=0.499; for E2E3 vs. E3E3: OR =1.33, 95%CI =1.12-1.42, P=0.008; for E2E4 vs. E3E3: OR =1.26, 95 % CI =1.07-1.49, P=0.005). CONCLUSION(S): The meta-analysis suggests an association between ApoE E4 mutation and increased risk of RPL.
Subject(s)
Abortion, Habitual/genetics , Apolipoproteins E/genetics , Genetic Association Studies , Polymorphism, Single Nucleotide/genetics , Abortion, Habitual/pathology , Alleles , Female , Genetic Predisposition to Disease , Genotype , Humans , Pregnancy , Risk FactorsABSTRACT
OBJECTIVE: To explore the differences of estrogen receptor α (ERα) gene polymorphism in patients with missed abortion and normal pregnancy and examine the relationship between ERα gene polymorphism and missed abortion. METHODS: A total of 100 cases of missed abortion patients and 102 cases of normal pregnant women were selected as the experimental and control groups. And 2ml blood samples and chorionic villus specimens were collected. The method of polymerase chain reaction restriction fragment length polymorphism was employed for ERα gene PvuII and XbaI polymorphism. And the data was analyzed to explore the relationship between ERα gene polymorphism and missed abortion. RESULTS: There was statistic significance in the frequency of ERα gene PvuII enzyme cleavage allele P and p from blood and villi samples between two groups, blood (χ(2) = 5.542, P < 0.05) OR: 1.742, villi (χ(2) = 7.559, P < 0.01), OR: 1.948. Statistic significances existed in the difference of frequency for ERα gene XbαI enzyme cleavage allele X and x from blood and villi samples between two groups, blood (χ(2) = 15.205, P < 0.01), OR:2.519; villi (χ(2) = 13.750, P < 0.01), OR: 2.499. There was a positive correlation of the frequency in ERα gene PvuII and XbαI genotype from blood and villi samples in the experimental group. CONCLUSIONS: It suggests that ERα gene polymorphism is correlated with the pathogenesis of missed abortion. Alleles P and X may be susceptibility genes.
Subject(s)
Abortion, Missed/genetics , Blood/metabolism , Chorionic Villi/metabolism , Estrogen Receptor alpha/genetics , Abortion, Missed/blood , Adult , Case-Control Studies , Female , Gene Frequency , Genotype , Humans , Polymorphism, Genetic , Pregnancy , Young AdultABSTRACT
In order to test whether 18S rDNA can influence positively xylanase gene effective expression in the yeast of Candida utilis, a targeting vector pGLR9K-XA was constructed by adding an interested gene xynA from Streptomyces olivaceoviridis into the vector pGLR9K which is constructed by ourselves. pGLR9K contains the 18S rDNA, GAP promoter and CYH resistance gene sequence, all of which is from C. utilis. Then the vector pGLR9K-XA was transformed into C. utilis. To test the vector and transformed system, PCR, Southern blot and DNS methods were used. The results showed that xylanase gene can be detected in the chromosome DNA of recombinant C. utilis and the enzyme activity of xylanase is up to 60 IU ml(-1) in the study. It is suggested that this system can be used to express exogenous genes in C. utilis as a bioreactors. This is the first report that xylanase gene was expressed in C. utilis.
