A Grating Interferometric Acoustic Sensor Based on a Flexible Polymer Diaphragm.

This study presents a grating interferometric acoustic sensor based on a flexible polymer diaphragm. A flexible-diaphragm acoustic sensor based on grating interferometry (GI) is proposed through design, fabrication and experimental demonstration. A gold-coated polyethylene terephthalate diaphragm was used for the sensor prototype. The vibration of the diaphragm induces a change in GI cavity length, which is converted into an electrical signal by the photodetector. The experimental results show that the sensor prototype has a flat frequency response in the voice frequency band and the minimum detectable sound pressure can reach 164.8 µPa/√Hz. The sensor prototype has potential applications in speech acquisition and the measurement of water content in oil. This study provides a reference for the design of optical interferometric acoustic sensor with high performance.

Temperature-robust optical microphone with a compact grating interferometric module.

The high demand for advanced acoustic sensors has prompted optical microphones to become a current research hotspot; this is especially the case in light of the performance of existing electroacoustic microphones having reached the ceiling. In this work, a thermally stable optical microphone has been developed for sensitive detection of low-frequency acoustic signals. The microphone was prepared using a prestressed nickel diaphragm and a compact grating interferometric module. The adjacent surfaces of the diaphragm and grating form a short Fabry-Perot cavity, which makes the microphone robust to ambient temperature fluctuation due to the reduced thermal drift of its operating point relative to the quadrature point of the interferometer. The cavity length-operating wavelength relationship of the microphone operating at the quadrature point was obtained. The performance of the prepared microphone was tested using various methods. Experimental results show that the microphone enables stable operation at the quadrature point over a wide range of temperatures from 0°C to 60°C with low signal distortion and high sensitivity. The response of the prepared optical microphone to low-frequency drone noise was measured and compared with that obtained with a commercial electret condenser microphone.

Prototype Optical Bionic Microphone with a Dual-Channel Mach-Zehnder Interferometric Transducer.

A prototype optical bionic microphone with a dual-channel Mach-Zehnder interferometric (MZI) transducer was designed and prepared for the first time using a silicon diaphragm made by microelectromechanical system (MEMS) technology. The MEMS diaphragm mimicked the structure of the fly Ormia Ochracea's coupling eardrum, consisting of two square wings connected through a neck that is anchored via the two torsional beams to the silicon pedestal. The vibrational displacement of each wing at its distal edge relative to the silicon pedestal is detected with one channel of the dual-channel MZI transducer. The diaphragm at rest is coplanar with the silicon pedestal, resulting in an initial phase difference of zero for each channel of the dual-channel MZI transducer and consequently offering the microphone strong temperature robustness. The two channels of the prototype microphone show good consistency in their responses to incident sound signals; they have the rocking and bending resonance frequencies of 482 Hz and 1911 Hz, and their pressure sensitivities at a lower frequency exhibit an "8"-shaped directional dependence. The comparison indicates that the dual-channel MZI transducer-based bionic microphone proposed in this work is advantageous over the Fabry-Perot interferometric transducer-based counterparts extensively reported.

Flexible hyperspectral surface plasmon resonance microscopy.

Optical techniques for visualization and quantification of chemical and biological analytes are always highly desirable. Here we show a hyperspectral surface plasmon resonance microscopy (HSPRM) system that uses a hyperspectral microscope to analyze the selected area of SPR image produced by a prism-based spectral SPR sensor. The HSPRM system enables monochromatic and polychromatic SPR imaging and single-pixel spectral SPR sensing, as well as two-dimensional quantification of thin films with the measured resonance-wavelength images. We performed pixel-by-pixel calibration of the incident angle to remove pixel-to-pixel differences in SPR sensitivity, and demonstrated the HSPRM's capabilities by using it to quantify monolayer graphene thickness distribution, inhomogeneous protein adsorption and single-cell adhesion. The HSPRM system has a wide spectral range from 400 nm to 1000 nm, an optional field of view from 0.884 mm2 to 0.003 mm2 and a high lateral resolution of 1.2 µm, demonstrating an innovative breakthrough in SPR sensor technology.

Fabrication of Glass Diaphragm Based Fiber-Optic Microphone for Sensitive Detection of Airborne and Waterborne Sounds.

A glass-diaphragm microphone was developed based on fiber-optic Fabry-Perot (FP) interferometry. The glass diaphragm was shaped into a wheel-like structure on a 150-µm-thick glass sheet by laser cutting, which consists of a glass disc connected to an outer glass ring by four identical glass beams. Such a structural diaphragm offers the microphone an open air chamber that reduces air damping and increases sensitivity and results in a cardioid direction pattern for the microphone response. The prepared microphone operates at 1550 nm wavelength, showing high stability in a range of temperature from 10 to 40 °C. The microphone has a resonance peak at 1152 Hz with a quality factor of 21, and its 3-dB cut-off frequency is 32 Hz. At normal incidence of 500 Hz sound, the pressure sensitivity of the microphone is 755 mV/Pa and the corresponding minimum detectable pressure is 251 µPa/Hz1/2. In addition to the above characteristics of the microphone in air, a preliminary investigation reveals that the microphone can also work stably under water for a long time due to the combination of the open-chamber and fiber-optic structures, and it has a large signal-to-noise ratio in response to waterborne sounds. The microphone prepared in this work is simple, inexpensive, and electromagnetically robust, showing great potential for low-frequency acoustic detection in air and under water.

Spatially Resolved Spectroscopic Characterization of Nanostructured Films by Hyperspectral Dark-Field Microscopy.

Nanostructured films have been widely used for preparing various advanced thin-film devices because of their unique electrical, optical, and plasmonic characteristics associated with the nano-size effect. In situ, nondestructive and high-resolution characterization of nanostructured films is essential for optimizing thin-film device performance. In this work, such thin-film characterization was achieved using a hyperspectral dark-field microscope (HSDFM) that was constructed in our laboratory by integrating a hyperspectral imager with a commercial microscope. The HSDFM allows for high-resolution (Δλ = 0.4 nm) spectral analysis of nanostructured samples in the visible-near-infrared region with a spatial resolution as high as 45 nm × 45 nm (corresponding to a single pixel). Four typical samples were investigated with the HSDFM, including the gold nanoplate array, the self-assembled gold nanoparticle (GNP) sub-monolayer, the sol-gel nanoporous titanium dioxide (TiO2) film, and the layer-stacked molybdenum disulfide (MoS2) sheet. According to the experimental results, the plasmon resonance scattering bands for nanoplate clusters are identical with those for individual gold nanoplates, indicating that the gap between adjacent nanoplates is too large to allow plasmonic coupling between them. A different case was observed with the self-assembled GNP sub-monolayer in which the aggregated clusters with the internal plasmonic interaction show a considerable red-shift of the plasmon resonance band relative to the isolated single GNP. In addition, the protein adsorption on the nanoporous TiO2 film was observed to be inhomogeneous on the microscale, and the stepped boundaries of the MoS2 sheet were clearly observed. A quasi-linear dependence of the single-pixel light intensity on the step height was obtained by combining the HSDFM with atomic force microscopy. The minimum thickness detectable by the present HSDFM is 6.5 nm, corresponding to the 10-layer MoS2 film. The work demonstrated the outstanding applicability of the HSDFM for nanostructured film characterization.

Gold-silver alloy film based surface plasmon resonance sensor for biomarker detection.

In this study, we developed a gold­silver alloy film based surface plasmon resonance (AuAg-SPR) sensor with wavelength interrogation to detect cancer antigen 125 (CA125) using a sandwich immunoassay. We first theoretically simulated the sensitivity of conventional gold film based SPR (Au-SPR) sensor and AuAg-SPR sensor, and conducted a series of experiments to investigate the sensitive characteristics of AuAg-SPR sensor, including the angle and refractive index (RI) sensitivity. We then conducted CA125 detection experiments on these two types of sensors. The results demonstrated that the limit of detection (LOD) of CA125 on the AuAg-SPR sensor was 0.1 U/mL (0.8 ng/mL) based on its direct reaction with an immobilised antibody, which was two orders of magnitude lower than that of the Au-SPR sensor (10 U/mL). The total changes in the resonance wavelength (∆λR) of the former were 1.7-fold those of the latter. The volume fractions of the adsorbates (fad) and effective RIs (nadlayer) in each adlayer were then calculated and the effect of the antibody size on the detection results was analysed. The AuAg-SPR sensors had a higher sensitivity than the conventional Au-SPR sensors for detecting CA125 due to their electric field characteristics. Therefore, these will have better application prospects.

A Potassium Ion-Exchanged Glass Optical Waveguide Sensor Locally Coated with a Crystal Violet-SiO2 Gel Film for Real-Time Detection of Organophosphorus Pesticides Simulant.

An optical waveguide (OWG) sensor was developed for real-time detection of diethyl chlorophosphate (DCP) vapor, which is a typical simulant for organophosphorus pesticides and chemical weapon agents. Silica gel, crystal violet (CV), and potassium ion-exchange (PIE) OWG were used to fabricate the sensor's device. In the real-time detection of the DCP vapor, the volume fraction of DCP vapor was recorded to be as low as 1.68 × 10-9. Moreover, the detection mechanism of CV-SiO2 gel film coated the PIE OWG sensor for DCP, which was evaluated by absorption spectra. These results demonstrated that the change of output light intensity of the OWG sensor significantly increased with the augment of the DCP concentration. Repeatability as well as selectivity of the sensors were tested using 0.042 × 10-6 and 26.32 × 10-6 volume fraction of the DCP vapor. No clear interference with the DCP detection was observed in the presence of other common solvents (e.g., acetone, methanol, dichloromethane, dimethylsulfoxide, and tetrahydrofuran), benzene series (e.g., benzene, toluene, chlorobenzene, and aniline), phosphorus-containing reagents (e.g., dimethyl methylphosphonate and trimethyl phosphate), acid, and basic gas (e.g., acetic acid and 25% ammonium hydroxide), which demonstrates that the OWG sensor could provide real-time, fast, and accurate measurement results for the detection of DCP.

AuAg alloy film-based colorful SPR imaging sensor for highly sensitive immunodetection of benzo[a]pyrene in water.

A colorful surface plasmon resonance imaging (SPRi) sensor with the hue-based enhanced sensitivity has been developed by using sputtered AuAg alloy thin film as the sensing layer. The condition for optimizing the hue-based sensitivity of the SPRi sensor was achieved, that is, the initial resonance wavelength is in the range from 595 to 610 nm. Under this condition, the hue-based refractive index sensitivity of the SPRi sensor was measured as high as Δhue/Δnc=29879/RIU. This sensitivity is 8 times higher than that obtained with a gold-layer SPRi sensor (Δhue/Δnc=3658/RIU) and 7.7 times as high in magnitude as the spectral sensitivity of the same sensor (ΔλR/Δnc=3897.6 nm/RIU). After functionalization of the AuAg alloy film with the monoclonal antibody, the sensor was used for quantitative immunodetection of highly carcinogenic benzo[a]pyrene (BaP) in water. According to the experimental results, the average hue of the SPR color image (300 pixels×300 pixels) experiences an initial rapid increase and then stabilizes 15 min after exposure of the functionalized AuAg alloy film to an aqueous BaP solution sample. The variation of average hue obtained at the equilibrium of surface immunoreaction is a linear function of BaP concentration with the slope being Δhue/Δc=132.2/(µg·L-1). A cooled CCD camera is able to distinguish a change of Δhue=1, offering the colorful SPRi sensor the BaP detection limit of 0.01 µg·L-1. The comparative measurements of the sensor's responses indicate that the hue variation obtained with 0.1 µg·L-1 of BaP is equal to that obtained either with 5 µg·L-1 of benzanthracene or with 5 µg·L-1 of benzofluoranthene, revealing the sensor's excellent specificity to BaP. The work demonstrated that the AuAg alloy film-based colorful SPRi sensor can be used not only for visualized analysis of molecular interaction at the surface but also for quantitative trace detection of small-molecule analytes.

An Optical MEMS Acoustic Sensor Based on Grating Interferometer.

Acoustic detection is of great significance because of its wide applications. This paper reports a Micro-Electro-Mechanical System (MEMS) acoustic sensor based on grating interferometer. In the MEMS structure, a diaphragm and a micro-grating made up the interference cavity. A short-cavity structure was designed and fabricated to reduce the impact of temperature on the cavity length in order to improve its stability against environment temperature variations. Besides this, through holes were designed in the substrate of the grating to reduce the air damping of the short-cavity structure. A silicon diaphragm with a 16.919 µm deep cavity and 2.4 µm period grating were fabricated by an improved MEMS process. The fabricated sensor chip was packaged on a conditioning circuit with a laser diode and a photodetector for acoustic detection. The output voltage signal in response to an acoustic wave is of high quality. The sensitivity of the acoustic sensor is up to -15.14 dB re 1 V/Pa @ 1 kHz. The output signal of the high-stability acoustic sensor almost unchanged as the environment temperature ranged from 5 °C to 55 °C.

Self-referenced antiresonant reflecting guidance mechanism for directional bending sensing with low temperature and strain crosstalk.

A sensitive one-dimensional vector bending fiber-optic sensor based on self-referenced antiresonant reflecting guidance mechanism has been proposed and experimentally demonstrated. Two symmetric air holes in the hollow-core photonic crystal fiber (HCPCF) were infiltrated with refractive index matching liquids with different refractive indices, which formed a self-referenced anti-resonant reflecting optical waveguide. The bending of the HCPCF induces a wavelength shift of lossy dip in the transmission spectrum. Specially, the one-dimensional bending orientation can be detected through the wavelength interval between two lossy dips due to the asymmetric refractive index change of the silica cladding for two resonators. The bending sensitivities are 4.86 and -4.84 nm/m-1 for the curvatures of the 0° and 180° bending orientations in a bending range from 0 to 0.88 m-1, respectively. Moreover, the temperature and strain crosstalk of the proposed sensor can be eliminated through the compensated self-referenced anti-resonant reflecting optical waveguide. The proposed fiber sensor can be used for the monitoring of the structural health of infrastructures.

Real-time fiber-optic anemometer based on a laser-heated few-layer graphene in an aligned graded-index fiber.

A real-time all-fiber anemometer based on laser-heated few-layer graphene in aligned graded-index fibers has been proposed and experimentally demonstrated. The proposed fiber-optic anemometer was composed of a pair of all-fiber collimators by using aligned graded-index fibers that was coated with the few-layer graphene. The few-layer graphene was heated through a heating light from a 532-nm laser, which changed the optical transmittance of signal light with the wavelength of 1550 nm. The wind speed can be measured through the transmission power of the signal light based on the wind cooling effects on the heated few-layer graphene, acting as a "hot-wire" anemometer. The experimental results show that the maximum sensitivity of the anemometer reaches -22.03 µW/(m/s), and a fast response time of as 0.064 s can be achieved. The proposed fiber sensor can be used for the real-time measurement of wind speed in the fields of environmental monitoring, oil exploration, oceanography research, etc.

Nanoporous Gold Films Prepared by a Combination of Sputtering and Dealloying for Trace Detection of Benzo[a]pyrene Based on Surface Plasmon Resonance Spectroscopy.

A wavelength-interrogated surface plasmon resonance (SPR) sensor based on a nanoporous gold (NPG) film has been fabricated for the sensitive detection of trace quantities of benzo[a]pyrene (BaP) in water. The large-area uniform NPG film was prepared by a two-step process that includes sputtering deposition of a 60-nm-thick AuAg alloy film on a glass substrate and chemical dealloying of the alloy film in nitric acid. For SPR sensor applications, the NPG film plays the dual roles of analyte enrichment and supporting surface plasmon waves, which leads to sensitivity enhancement. In this work, the as-prepared NPG film was first modified with 1-dodecanethiol molecules to make the film hydrophobic so as to improve BaP enrichment from water via hydrophobic interactions. The SPR sensor with the hydrophobic NPG film enables one to detect BaP at concentrations as low as 1 nmol·L-1. In response to this concentration of BaP the sensor produced a resonance-wavelength shift of ΔλR = 2.22 nm. After the NPG film was functionalized with mouse monoclonal IgG1 that is the antibody against BaP, the sensor's sensitivity was further improved and the BaP detection limit decreased further down to 5 pmol·L-1 (the corresponding ΔλR = 1.77 nm). In contrast, the conventional SPR sensor with an antibody-functionalized dense gold film can give a response of merely ΔλR = 0.9 nm for 100 pmol·L-1 BaP.

Algorithmic Enhancement of Spectral Resolution of a LiNbO3 Waveguide-Based Miniature Fourier Transform Spectrometer.

In a recent report we demonstrated a miniature static Fourier transform spectrometer (FTS) that was implemented with a LiNbO3 (LN) waveguide electro-optic modulator (EOM) combined with the dispersion relation between its half-wave voltage and wavelength. The FTS was verified to be able to measure laser wavelength and for low-resolution spectroscopy. In this report, we successfully applied the resolution enhancement algorithm to the FTS, resulting in at least a three-fold increase in its spectral resolution without causing obvious distortion of the measured spectra. The algorithm method used is based on an autoregressive (AR) model, singular value decomposition (SVD), and forward-backward linear prediction (FBLP). The combination of these methods allows the FTS to remain a small size but to possess good spectral resolution, effectively mitigating the conflict between the small size and high resolution of the device. This study opens the way to development of high-resolution miniature FTS.

Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler.

An optical fiber optofluidic biosensor for the detection of DNA hybridization and methylation has been proposed and experimentally demonstrated. An in-line fiber Michelson interferometer was formed in the photonic crystal fiber. A micrhole in the collapsed region, which combined the tunable mode coupler and optofluidic channel, was fabricated by using femtosecond laser micromachining. The mode field diameter of the guided light is changed with the refractive index in the optofluidic channel, which results in the tunable coupling ratio. Label-free detections of the DNA hybridization and methylation have been experimentally demonstrated. The probe single stranded DNA (ssDNA) was bound with the surface of the optofluidic channel through the Poly-l-lysine layer, and the hybridization between a short 22-mer probe ssDNA and a complementary target ssDNA was carried out and detected by interrogating the fringe visibility of the reflection spectrum. Then, the DNA methylation was also detected through the binding between the methylated DNA and the 5-methylcytosine (5-mC) monoclonal antibody. The experiments results demonstrate that the limit of detection of 5nM is achieved, establishing the tunable mode coupler as a sensitive and versatile biosensor. The sensitive optical fiber optofluidic biosensor possesses high specificity and low temperature cross-sensitivity.

A prototype stationary Fourier transform spectrometer for near-infrared absorption spectroscopy.

A prototype stationary Fourier transform spectrometer (FTS) was constructed with a fiber-coupled lithium niobate (LiNbO3) waveguide Mach-Zehnder interferometer (MZI) for the purpose of rapid on-site spectroscopy of biological and chemical measurands. The MZI contains push-pull electrodes for electro-optic modulation, and its interferogram as a plot of intensity against voltage was obtained by scanning the modulating voltage from -60 to +60 V in 50 ms. The power spectrum of input signal was retrieved by Fourier transform processing of the interferogram combined with the wavelength dispersion of half-wave voltage determined for the MZI used. The prototype FTS operates in the single-mode wavelength range from 1200 to 1700 nm and allows for reproducible spectroscopy. A linear concentration dependence of the absorbance at λmax = 1451 nm for water in ethanolic solution was obtained using the prototype FTS. The near-infrared spectroscopy of solid samples was also implemented, and the different spectra obtained with different materials evidenced the chemical recognition capability of the prototype FTS. To make this prototype FTS practically applicable, work on improving its spectral resolution by increasing the maximum optical path length difference is in progress.

In situ study of self-assembled nanocomposite films by spectral SPR sensor.

Spectral surface plasmon resonance (SPR) sensor with a time-resolved charge-coupled device (CCD) detector is a powerful analytical tool for label-free detection of biomolecular interaction at the liquid/solid interface and for in situ study of molecular adsorption behavior. In this work, the layer-by-layer self-assembly processes for three nanocomposite films were monitored in real time using a broadband spectral SPR sensor with a large dynamic range. Kinetics studies suggest that cytochrome c (Cyt c) and deoxy ribonucleic acid (DNA) adsorptions obey the Langmuir-isotherm theory, while gold nanoparticle (GNP) adsorption follows the Diffusion-controlled model. Using poly(sodium 4-styrenesulfonate) (PSS) and poly(dimethyldiallylammonium chloride) (PDDA) as the positively charged agents, three kinds of multilayer films such as the PSS/Cyt c, GNP/Cyt c and PDDA/DNA binary nanocomposites were fabricated on the SPR chips by the electrostatic attraction based on self-assemble. The SPR response in terms of ΔλR was measured to linear increase with increasing the number of layers for a six-bilayer PSS/Cyt c nanocomposite film, indicating that every PSS/Cyt c layer has equal mass coverage. In contrast, the nonlinear dependences of ΔλR on the number of bilayers were observed for the GNP/Cyt c and PDDA/DNA nanocomposite multilayer films.

Waveguide-coupled directional Raman radiation for surface analysis.

Kretschmann-type waveguide structures, including Plasmon Waveguide (PW) and Resonant Mirror (RM), have been applied in interfacial Raman spectroscopy due to the following unique features: (1) unlike the classic surface enhanced Raman scattering (SERS) substrates made of either gold or silver, both PW and RM can be prepared using a large variety of inexpensive materials; (2) the field enhancement factors using these structures can be theoretically predicted and experimentally controlled, which enables us to manipulate the surface Raman sensitivity with high repeatability; (3) the use of transverse electric (TE) and transverse magnetic (TM) modes for Raman excitation allows us to evaluate the orientation of target molecules immobilized on the waveguide surface; (4) the unwanted impact of noble metals on the Raman fingerprints of target molecules, which is often observed for conventional SERS substrates, can be avoided upon the use of dielectric waveguides. In this paper, guided-mode-coupled directional Raman emission, which is an additional important feature of the waveguide Raman technique, was theoretically investigated based on the optical reciprocity theorem combined with the Fresnel equations. The simulation results indicate that the directional Raman emission from a dipole located within the field confinement and penetration depth of a guided mode depends on both the orientation of the dipole and its distance from the waveguide surface. Raman light from the TE-oriented dipoles is launched into the prism coupler at the TE-mode resonance angle and that from the non-TE-oriented dipoles propagates at the TM-mode resonance angle. The intensity of the guided-mode-excited Raman signal propagating at the mode resonance angle is proportional to the fourth power of the mode field (E(4)) at the depth of the dipole from the waveguide surface. This means that the guided-mode-excited and guided-mode-coupled directional Raman spectroscopy has a detection depth that is as small as a quarter of the evanescent-field penetration depth, indicating the excellent surface selectivity of this technique. The directional Raman emission also facilitates high-efficiency signal collection compared with conventional SERS. It is worth noting that Raman light from the dipoles confined in the core layer of a single-mode waveguide can be simultaneously coupled into both the guided mode and the substrate mode, especially the surface plasmon resonance (SPR) mode for PW.

Miniature Fourier transform spectrometer based on wavelength dependence of half-wave voltage of a LiNbO3 waveguide interferometer.

A simple and reliable spectrum-retrieval method was proposed for the development of miniature stationary Fourier transform (FT) spectrometers based on a LiNbO3 (LN) waveguide Mach-Zehnder interferometer (MZI) modulator. The method takes into account the wavelength dependence of the optical pathlength difference (OPD) and allows us to use a nonlinear voltage ramp to modulate the OPD. The method is based on the dispersion of the half-wave voltage, which was measured to be a monotonous polynomial function of the wavelength for the LN waveguide MZI used. With the measured dispersion of the half-wave voltage, the OPD, as a linear function of the modulating voltage, can be accurately determined at each wavelength in the near-infrared region in which the MZI used is a single-mode device. A prototype FT spectrometer was prepared using a LN waveguide MZI modulator based on the above method. The experimental results demonstrated that the spectrometer can be used for accurate determination of the laser wavelength and for liquid absorptiometry.

Wavelength-interrogated surface plasmon resonance sensor with mesoporous-silica-film-enhanced sensitivity to small molecules.

Sol-gel copolymer-templated mesoporous silica films with a thickness of 70 nm and interpore spacing of 4.34 nm were fabricated on gold layer covered glass substrates for application as a wavelength-interrogated surface plasmon resonance (SPR) sensor. The resonance wavelength (λ(R)) of the sensor with a solution sample was determined by absorptiometry at a given incident angle. A comparison between the experimental data obtained with the coated and uncoated SPR chips demonstrated that the mesoporous silica film effectively enhanced sensor response to individual adsorption of cysteamine molecules and lead(II) ions. An approximate proportional relationship between the resonance-wavelength shift of the sensor and the volume fraction of analyte molecules adsorbed in the mesoporous silica film was obtained by numerical simulation. Porosities of 0.865 and 0.785 for the two silica films used as well as the volume fractions of 0.048 and 0.116 for adsorbed lysozyme and cysteamine molecules were determined by fitting the simulation results to the experimental data. The adsorbed amount of cysteamine (∼0.5 nm) is equivalent to more than 16 full monolayers on the geometric surface of the mesoporous silica film used. In contrast, an equivalence of less than 2 full monolayers for adsorbed lysozyme molecules (3 nm × 3 nm × 4.5 nm) suggests that the mesoporous silica film has good size-selective adsorption capability due to its uniform pore size distribution. Cysteamine modification of the mesoporous silica film renders the SPR sensor able to detect lead(II) ions at concentrations as low as 1 nM.