[Basic Research on Therapy of Leukemia with Nanoparticles Drug Delivery System Modified by Transferrin Receptor Monoclonal Antibody].

OBJECTIVE: To investigate the therapeutic effect of targeted drug-loaded nanoparticles modified by transferrin receptor monoclonal antibody (TfR mAb) on acute leukemia and its potential anti-tumor mechanism. METHODS: Nanoparticles drug delivery system, which was composed of poly (lactic-co-glycolic acid), poly-l-lysine, polyethylene glycol, TfR mAb (TfR mAb-PLGA-PLL-PEG)-daunorubicin (DNR), was first synthesized. After drug intervention, the intracellular accumulation in leukemia HL60 cells was observed under a fluorescent microscope and concentration of DNR was determined by flow cytometry (FCM). Meanwhile, cell apoptosis rate was measured by FCM and the expression levels of apoptosis related protein Cleaved-caspase 3 was determined by Western blot. RESULTS: Under an inverted fluorescent microscope, intracellular accumulation of DNR autofluorescence in HL60 cells was observed in both TfR mAb-PLGA-PLL-PEG-DNR group and DNR group. FCM analysis showed that the intracellular concentration of DNR in TfR mAb-PLGA-PLL-PEG-DNR group was higher than that in DNR group（P<0.05）. The apoptotic rate of HL60 cells in TfR mAb-PLGA-PLL-PEG-DNR group was higher than that of DNR group（P<0.05）. Moreover, the expression levels of apoptosis-related protein Cleaved-caspase 3 in TfR mAb-PLGA-PLL-PEG-DNR group was significantly higher than that in DNR group（P<0.05）. CONCLUSION: TfR mAb-PLGA-PLL-PEG nanoparticle drug delivery system can target chemotherapy drugs to leukemia cells and enhance anticancer ability through apoptotic pathway.

Numerical investigation of the thermal effect on flow and dispersion of rooftop stack emissions with wind tunnel experimental validations.

The thermal effect on the flow and dispersion of pollutants emitted from a rooftop stack is investigated by means of CFD (computational fluid dynamics) models with wind tunnel experimental validations. The leeward wall and its nearby ground are heated simultaneously to mimic solar radiation. Seventeen Ri (Richardson number) cases with four inflow wind speeds (1, 3, 6, and 9 m/s) and five temperature differences (0, 60, 120, 180, and 240 K) between the heated surface and ambient air are considered to represent the interaction between thermal buoyancy force and inertia force. The results reveal that (1) the steady RANS (Reynolds Averaged Navier-Stokes) computations with Boussinesq approximation can generally reproduce the effect of thermal buoyancy on the wake flow and pollutant distribution in wind tunnel experiments; (2) the wake vortex flow is less affected by the thermal buoyancy force at small Ri (e.g., Ri ≤ 0.26) while an upward flow rather than a clockwise vortex structure is developed in the near wake at Ri ≥ 0.58; (3) it is inappropriate to place fresh air intakes on the leeward wall of the emitting building, but natural ventilation through windows on the leeward wall can be implemented at higher Ri (e.g., Ri = 2.33); (4) at the pedestrian respiration height downstream of the building, the distance between the location of maximum pollutant concentration and the leeward wall increases linearly with Ri while the maximum dimensionless concentration decreases exponentially with increasing Ri; (5) the air temperature is rapidly reduced away from the heated wall/ground and a heat accumulation zone is formed at the ground corner next to the leeward wall. This study can be helpful for determining the strategy for natural ventilation through windows and for evaluating the impacts of rooftop stack exhaust on air quality downstream of emitting buildings.

Relationship between emotional intelligence trait anger and aggressive behavior of middle school students with childhood psychological abuse / 中国学校卫生

Objective@#To explore the intrinsic mechanism of emotional intelligence and trait anger in the association between childhood psychological abuse and aggressive behavior among middle school students,and to provide a theoretical basis for intervention of aggressive behaviors.@*Methods@#By using the multi-stage stratified random sampling method, 2 458 middle school students in Guizhou Province were selected, and group tests were carried out using psychological abuse scale, attack behavior scale,emotional intelligence scale and characteristic anger scale.@*Results@#Agressive behavior, trait anger and emotional intelligence differed significantly by gender(t=2.19，5.12，-2.34，P<0.05), and there were significant differences in aggressive behavior, psychological abuse, emotional intelligence and trait anger among middle school students of different student origins by residence(t=-18.77，-6.04，9.10，-9.94，P<0.05). Childhood psychological abuse (r=0.41), temperamental trait anger (r=0.52) and reactive trait anger (r=0.49) were significantly positively correlated with aggressive behavior（P<0.05）. Emotional intelligence was significantly negatively correlated with aggressive behavior (r=-0.33, P<0.05). The test of multiple mediation effects showed that emotional intelligence, temperamental trait anger and reactive trait anger played multiple mediation roles between psychological abuse and aggressive behavior in childhood.@*Conclusion@#Childhood psychological abuse could not only directly affect the aggressive behavior of middle school students, but also further affect the aggressive behavior through emotional intelligence and trait anger. Emotional intelligence and idiosyncratic anger are important internal mechanisms by which childhood psychological abuse affects aggressive behavior.