ABSTRACT
BACKGROUND: Till date only a few studies have reported the efficacy and clinical improvements obtained by extracorporeal shock-wave therapy (ESWT) on frozen shoulder. Limited by small number of studies and insufficient outcomes, it is important and necessary to conduct a new randomized controlled trial. The purpose of the present study is to determine whether ESWT could be more effective than oral steroid in treatment of frozen shoulder. METHODS: This randomized, single-blind, superiority clinical trial was approved by the institutional review board in The Third People's Hospital of Linyi. The inclusion criteria were patients aged >18 years with shoulder pain and restriction in range of motion. A symptom duration >3 months was required, with no radiographic findings on anteroposterior shoulder plain radiographs except for osteoporosis. Group 1 patients were given 30âmg of oral prednisolone daily for 2 weeks as a single morning dose and then 15âmg daily for another 2 weeks. Group 2 patients received 3 sessions of ESWT on the first, 14th, and 28th days. The primary outcome measure was shoulder pain score. The secondary outcomes included Disabilities of the Arm, Shoulder, and Hand score, range of motion, satisfaction rate, and complications. RESULTS: It was hypothesized that there would be a significant difference between ESWT and control groups in improving shoulder pain and functions in frozen shoulder. TRIAL REGISTRATION: This study protocol was registered in Research Registry (researchregistry5736).
Subject(s)
Bursitis/therapy , Extracorporeal Shockwave Therapy , Adult , Aged , Female , Humans , Male , Middle Aged , Pain Measurement , Range of Motion, Articular , Single-Blind Method , Treatment Outcome , Young AdultABSTRACT
Long noncoding RNA (lncRNA) LINC00473 plays a carcinogenic role in a variety of different tumor types. Nevertheless, the mechanisms through which LINC00473 regulates the radiosensitivity of esophageal squamous cell carcinoma (ESCC) cells remains elusive. In the present study, reverse transcriptionquantitative PCR was used to quantify the expression of LINC00473, microRNA (miRNA/miR)4975p and cell division cycle 25A (CDC25A) in ESCC tissues. The association between LINC00473 expression and the clinicopathological characteristics of patients with ESCC was also assessed. Furthermore, Cell Counting kit8 and colony formation assays were carried out to monitor the proliferation of ESCC cells exposed to Xray radiation. A dualluciferase reporter assay was also conducted to analyze the interaction between LINC00473 and miR4975p, as well as the interaction between CDC25A and miR4975p. The findings of the present study demonstrated that in ESCC tissues and cells, the expression levels of LINC00473 and CDC25A were significantly upregulated, while the expression of miR4975p was downregulated. The high expression level of LINC00473 was associated with a higher T stage, lymph node metastasis stage and a lower tumor differentiation grade in patients with ESCC. Following irradiation, transfection with miR4975p mimics reduced the promoting effect of LINC00473 overexpression on ESCC cell proliferation, and partially impeded the resistance of ESCC cells to Xray radiation induced by LINC00473 overexpression. Moreover, transfection with miR4975p inhibitors partially alleviated the inhibitory effects of LINC00473 knockdown on cellular proliferation, and partly reversed the sensitivity of cells to Xray irradiation induced by LINC00473 knockdown. Furthermore, it was confirmed that miR4975p was able to bind LINC00473 and the 3'untranslated region of CDC25A. On the whole, the findings of the present study demonstrate that LINC00473 reduces the radiosensitivity of ESCC cells by modulating the miR4975p/CDC25A axis.
Subject(s)
Esophageal Neoplasms/metabolism , Esophageal Neoplasms/radiotherapy , Esophageal Squamous Cell Carcinoma/metabolism , Esophageal Squamous Cell Carcinoma/radiotherapy , MicroRNAs/metabolism , RNA, Long Noncoding/metabolism , cdc25 Phosphatases/metabolism , 3' Untranslated Regions/genetics , Blotting, Western , Cell Division/genetics , Cell Division/physiology , Cell Line , Computational Biology , Esophageal Neoplasms/genetics , Esophageal Squamous Cell Carcinoma/genetics , Female , Humans , Male , MicroRNAs/genetics , Middle Aged , RNA, Long Noncoding/genetics , Radiation Tolerance/genetics , Radiation Tolerance/physiology , Reverse Transcriptase Polymerase Chain Reaction , cdc25 Phosphatases/geneticsABSTRACT
Parkinson's disease (PD), the second most prevalent age-related neurodegenerative disease, occurs as a result of the loss of dopaminergic neurons in the substantia nigra. Long non-coding RNA-p21 (lnc-p21) has been demonstrated to be upregulated in PD. However, its role in PD is unknown. Here, the results showed that lnc-p21 was highly expressed in human neuroblastoma SH-SY5Y cells treated with MPP+. Knockdown of lnc-p21 attenuated the cytotoxicity and cell apoptosis induced by MPP+ as shown by enhanced cell viability, decreased LDH release and cell apoptosis rate, accompanying with reduction of caspase-3 activity and Bax expression, and enhancement of Bcl-2 expression. Furthermore, knockdown of lnc-p21 mitigated MPP+-induced oxidative stress and neuroinflammation, as evidenced by the decrease in ROS generation, increase in SOD activity and decline in TNF-α, IL-1ß and IL-6 levels. Conversely, overexpression of lnc-p21 resulted in the opposite effect. miR-625 was identified as a target of lnc-p21. lnc-p21 overturned the inhibitory effect of miR-625 on MPP+-induced neuronal injury in SH-SY5Y cells. Additionally, lnc-p21 positively regulated TRPM2 expression by targeting miR-625, and knockdown of TRPM2 inhibited MPP+-induced neuronal injury. Overall, our study identified a new lnc-p21-miR-625-TRPM2 regulatory network that lnc-p21 regulated MPP + -induced neuronal injury by sponging miR-625 and upregulating TRPM2 in SH-SY5Y cells, which provide a better understanding for the pathogenesis of PD.