ABSTRACT
Circular RNAs (circRNAs) have been identified as important regulators in cancer progression. Nevertheless, little is known about the biological function of circ_0000376 in the progression of osteosarcoma (OS). Cell viability, colony formation ability, apoptosis, and motility were analyzed by Cell Counting Kit-8 assay, colony formation assay, flow cytometry, and transwell assays. Cellular glycolytic metabolism was analyzed using commercial kits. RT-qPCR and Western blot assay were performed to analyze RNA and protein expression in OS tissues and cells. Starbase software was used to establish circRNA-microRNA (miRNA)-messenger RNA linkage, and intermolecular interaction was verified by dual-luciferase reporter assay. Xenograft tumor assay was conducted to analyze the effects of Tanshinone I (Tan I) and circ_0000376 on xenograft tumor growth in vivo. Tan I treatment suppressed the viability, migration, invasion, and glycolysis and triggered the apoptosis of OS cells. Tan I treatment markedly down-regulated circ_0000376 expression in OS cells. The addition of circ_0000376 plasmid largely rescued the malignant behaviors of OS cells upon Tan I exposure. Circ_0000376 interacted with miR-432-5p in OS cells. Circ_0000376 overexpression-mediated protective effects in Tan I-induced OS cells were partly attenuated by the accumulation of miR-432-5p. miR-432-5p bound to the 3' untranslated region (3'UTR) of B-cell leukemia/lymphoma 2 (BCL2) in OS cells. miR-432-5p interference-induced effects in Tan I-treated OS cells were partly overturned by the silence of BCL2. Circ_0000376 can act as miR-432-5p sponge to up-regulate BCL2 expression in OS cells. Circ_0000376 silencing contributed to the anti-tumor effect of Tan I on the growth of xenograft tumors in vivo. Tan I exerted an anti-tumor role in OS progression by targeting circ_0000376/miR-432-5p/BCL2 axis.
Subject(s)
Abietanes/pharmacology , Bone Neoplasms/metabolism , MicroRNAs/metabolism , Osteosarcoma/metabolism , Proto-Oncogene Proteins c-bcl-2/metabolism , RNA, Circular/metabolism , RNA, Neoplasm/metabolism , Signal Transduction , Animals , Bone Neoplasms/drug therapy , Bone Neoplasms/genetics , Bone Neoplasms/pathology , Cell Line, Tumor , Humans , Mice , Mice, Inbred BALB C , Mice, Nude , MicroRNAs/genetics , Osteosarcoma/drug therapy , Osteosarcoma/genetics , Osteosarcoma/pathology , Proto-Oncogene Proteins c-bcl-2/genetics , RNA, Circular/genetics , RNA, Neoplasm/genetics , Xenograft Model Antitumor AssaysABSTRACT
Objective:To develop a patient-reported outcomes (PRO) scale for post-stroke aphasia based on the Traditional Chinese Medicine (TCM) holism. Methods:Referring to the TCM holism, the theoretical model was established following the standard process for the development of the PRO scale. An item pool was established and optimized with case review, patient interview, expert questionnaire survey and consensus conference. The PRO scale was established finally. Results:A pool of post-stroke aphasia items based on the holistic view of TCM was constructed in the physical, psychological and social model. The Expert Comment Form for the PRO Scale for Aphasia after Stroke was formed after sorting out the item pool. After expert surveys and meeting discussion, 40 items were finally selected to form the first version of Patient-reported Outcomes Scale for Post-stroke Aphasia-TCM. Conclusion:The first version of Patient-reported Outcomes Scale for Post-stroke Aphasia-TCM has been developed based on the TCM holism and the concept of PRO, which can be evaluated clinically.
ABSTRACT
Objective:To clarify the characteristics of electroencephalogram (EEG) power spectrum network of right hemisphere in patients with post-stroke aphasia (PSA) after left brain damaged. Methods:From December, 2018 to June, 2019, twelve PSA patients with left hemisphere injury were recruited and twelve healthy adults were matched as control group. China Rehabilitation Research Center aphasia examination (CRRCAE) was used to evaluated the linguistic function, and the EEG was collected. The functional connection characteristics of Alpha and Theta power spectrum were compared, and the correlation with language items was analyzed. Results:In PSA patients, the functional connection enhanced in Alpha and Theta frequency bands of central area, frontal, parietal and frontal-parietal joint areas, and decreased in Theta frequency bands of temporoparietal, parietal-occipital area, frontal, fronta-parietal and frontal central areas. The enhancement of alpha frequency functional connection from the right parietal occipital region to the central region was significantly correlated to the reduction of the expression ability (P < 0.05), while the weakening of theta frequency functional connection between the right parietal occipital region and the forehead and the center of the forehead was significantly correlated to the ability of speaking, reading, copying, dictation and calculation (r = -0.676~-0.717, P < 0.05). Conclusion:EEG power spectrum network can reflect the reorganization of right brain function network, and the change of right frontal parietal central network function connection may be closely related to PSA language injury and recovery.
ABSTRACT
OBJECTIVE@#To compare and analyse the efficiency of the low-temperature plasma radiofrequency and the traditional method in tonsillectomy.@*METHOD@#Sixty-four patients with chronic tonsillitis were randomly divided into group A (39 cases) and group B (25 cases). Patients in the group A were treated with traditional method tonsillectomy while patients in the group B were treated with low-temperature plasma radio frequency tonsillectomy. Analysed differences in operation time, blood loss, and postoperative pain as well as the incidence of postoperative between two groups.@*RESULT@#There were significant differences in operative time, blood loss, and postoperative pain (P 0.05).@*CONCLUSION@#Low-temperature plasma radiofrequency tonsillectomy is a safer method and has many superiorities including shorter operative time, less blood loss, quicker recovery of postoperative pain compared with traditional tonsillectomy.
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Cold Temperature , Hypothermia, Induced , Tonsillectomy , Methods , Tonsillitis , General SurgeryABSTRACT
OBJECTIVE@#To compare the three different approaches in the curettage of adenoid hypertrophy: the traditional curettage of adenoids through mouth (group A), the adenoidectomy through nose under nasal endoscope (group B), the adenoidectomy through both mouth and nose under nasal endoscope (group C).@*METHOD@#Clinical data of adenoid hypertrophy patients who had accepted the surgery therapy, were retrospectively analyzed; Symptoms of night snore, nasal obstruction, rhinorrhea, acouesthesia and the adenoid residue among the patients who had got three different surgery, were compared among them.@*RESULT@#Adenoid residue were found in six cases of group A. No adenoid residue were found in group B and C . Recovery of nasal obstruction and night snore in group C were much better than that in group A and B (P<0.01, <0.05).@*CONCLUSION@#The ideal way to cure adenoidal hypertrophy is the adenoidectomy done through mouth and nose under nasal endoscope.
