Design of GPU Network-on-Chip for Real-Time Video Super-Resolution Reconstruction.

Deep learning has a better output quality compared with traditional algorithms for video super-resolution (SR), but the network model needs large resources and has poor real-time performance. This paper focuses on solving the speed problem of SR; it achieves real-time SR by the collaborative design of a deep learning video SR algorithm and GPU parallel acceleration. An algorithm combining deep learning networks with a lookup table (LUT) is proposed for the video SR, which ensures both the SR effect and ease of GPU parallel acceleration. The computational efficiency of the GPU network-on-chip algorithm is improved to ensure real-time performance by three major GPU optimization strategies: storage access optimization, conditional branching function optimization, and threading optimization. Finally, the network-on-chip was implemented on a RTX 3090 GPU, and the validity of the algorithm was demonstrated through ablation experiments. In addition, SR performance is compared with existing classical algorithms based on standard datasets. The new algorithm was found to be more efficient than the SR-LUT algorithm. The average PSNR was 0.61 dB higher than the SR-LUT-V algorithm and 0.24 dB higher than the SR-LUT-S algorithm. At the same time, the speed of real video SR was tested. For a real video with a resolution of 540×540, the proposed GPU network-on-chip achieved a speed of 42 FPS. The new method is 9.1 times faster than the original SR-LUT-S fast method, which was directly imported into the GPU for processing.

Prenatal triclosan exposure impairs mammalian lung branching morphogenesis through activating Bmp4 signaling.

Triclosan (TCS) is a commonly used antibacterial agent present in personal care and household products. Recently, there have been increasing concerns about the association between children's health and TCS exposure during gestation, but the toxicological effects of TCS exposure on embryonic lung development remain undetermined. In this study, through using an ex vivo lung explant culture system, we found that prenatal exposure to TCS resulted in impaired lung branching morphogenesis and altered proximal-distal airway patterning. These TCS-induced dysplasias are accompanied by significantly reduced proliferation and increased apoptosis within the developing lung, as a consequence of activated Bmp4 signaling. Inhibition of Bmp4 signaling by Noggin partially rescues the lung branching morphogenesis and cellular defects in TCS-exposed lung explants. In addition, we provided in vivo evidence that administration of TCS during gestation leads to compromised branching formation and enlarged airspace in the lung of offspring. Thus, this study provides novel toxicological information on TCS and indicated a strong/possible association between TCS exposure during pregnancy and lung dysplasia in offspring.

Nuclear m6A reader YTHDC1 promotes muscle stem cell activation/proliferation by regulating mRNA splicing and nuclear export.

Skeletal muscle stem cells (also known as satellite cells [SCs]) are essential for muscle regeneration and the regenerative activities of SCs are intrinsically governed by gene regulatory mechanisms, but the post-transcriptional regulation in SCs remains largely unknown. N(6)-methyladenosine (m6A) modification of RNAs is the most pervasive and highly conserved RNA modification in eukaryotic cells; it exerts powerful impact on almost all aspects of mRNA processing that is mainly endowed by its binding with m6A reader proteins. In this study, we investigate the previously uncharacterized regulatory roles of YTHDC1, an m6A reader in mouse SCs. Our results demonstrate that YTHDC1 is an essential regulator of SC activation and proliferation upon acute injury-induced muscle regeneration. The induction of YTHDC1 is indispensable for SC activation and proliferation; thus, inducible YTHDC1 depletion almost abolishes SC regenerative capacity. Mechanistically, transcriptome-wide profiling using LACE-seq in both SCs and mouse C2C12 myoblasts identifies m6A-mediated binding targets of YTHDC1. Next, splicing analysis defines splicing mRNA targets of m6A-YTHDC1. Furthermore, nuclear export analysis also leads to the identification of potential mRNA export targets of m6A-YTHDC1 in SCs and C2C12 myoblasts;interestingly, some mRNAs can be regulated at both splicing and export levels. Lastly, we map YTHDC1 interacting protein partners in myoblasts and unveil a myriad of factors governing mRNA splicing, nuclear export, and transcription, among which hnRNPG appears to be a bona fide interacting partner of YTHDC1. Altogether, our findings uncover YTHDC1 as an essential factor controlling SC regenerative ability through multifaceted gene regulatory mechanisms in mouse myoblast cells.

A one-sided exponentially weighted moving average control chart for time between events.

Exponentially weighted moving average (EWMA) control charts for time-between-events (TBE) are commonly suggested to monitor high-quality processes for the early detection of process deteriorations. In this study, an enhanced one-sided EWMA TBE scheme is developed for rapid detection of increases or decreases in the process mean. The use of the truncation method helps to improve the sensitivity of the proposed scheme in the process mean detection. Moreover, by taking the effects of parameter estimation into account, the proposed scheme with estimated parameters is also investigated. Both the average run length (ARL) and standard deviation of run length (SDRL) performances of the proposed scheme with known and estimated parameters are studied using the Markov chain method, respectively. Furthermore, an optimal design procedure is developed for the recommended one-sided EWMA TBE chart based on ARL. Numerical results show that the proposed optimal one-sided EWMA TBE chart is more sensitive than the existing optimal one-sided exponential EWMA chart in monitoring both upward and downward mean shifts. Meanwhile, it also performs better than the existing comparative scheme in resisting the effect of parameter estimation. Finally, two illustrative examples are considered to show the implementation of the proposed scheme for simulated and real datasets.

seRNA PAM controls skeletal muscle satellite cell proliferation and aging through trans regulation of Timp2 expression synergistically with Ddx5.

Muscle satellite cells (SCs) are responsible for muscle homeostasis and regeneration and lncRNAs play important roles in regulating SC activities. Here, in this study, we identify PAM (Pax7 Associated Muscle lncRNA) that is induced in activated/proliferating SCs upon injury to promote SC proliferation as myoblast cells. PAM is generated from a myoblast-specific super-enhancer (SE); as a seRNA it binds with a number of target genomic loci predominantly in trans. Further studies demonstrate that it interacts with Ddx5 to tether PAM SE to its inter-chromosomal targets Timp2 and Vim to activate the gene expression. Lastly, we show that PAM expression is increased in aging SCs, which leads to enhanced inter-chromosomal interaction and target genes upregulation. Altogether, our findings identify PAM as a previously unknown lncRNA that regulates both SC proliferation and aging through its trans gene regulatory activity.

Ordered-Porous-Array Polymethyl Methacrylate Films for Radiative Cooling.

Passive radiative cooling is a spontaneous pattern of reflecting sunlight and radiating heat into the cold outer space through transparent atmosphere windows. In this work, an ordered-porous-array polymethyl methacrylate (OPA-PMMA) film with the properties of excellent radiative cooling is designed and studied. An ultra-high emissivity of 98.4% in the mid-infrared region (3-25 µm) and a good solar reflectance of 85% in the ultraviolet and near-infrared solar spectra (0.2-2.5 µm) were achieved. The surface temperature of the OPA-PMMA film is 16 °C lower than that of the smooth-surface PMMA films and is 8.6 °C lower than that of the commercial white paint in the outdoor test. The structure of the OPA plays an important role in improving solar reflectivity and emissivity. The films are fabricated using a one-step low-cost process that can be applied for large-scale production. It is vital for promoting radiative cooling as a viable energy technology for buildings, fabric, or equipment that need a cooling environment.

GM130 regulates pulmonary surfactant protein secretion in alveolar type II cells.

Pulmonary surfactant is a lipid-protein complex secreted by alveolar type II epithelial cells and is essential for the maintenance of the delicate structure of mammalian alveoli to promote efficient gas exchange across the air-liquid barrier. The Golgi apparatus plays an important role in pulmonary surfactant modification and secretory trafficking. However, the physiological function of the Golgi apparatus in the transport of pulmonary surfactants is unclear. In the present study, deletion of GM130, which encodes for a matrix protein of the cis-Golgi cisternae, was shown to induce the disruption of the Golgi structure leading to impaired secretion of lung surfactant proteins and lipids. Specifically, the results of in vitro and in vivo analysis indicated that the loss of GM130 resulted in trapping of Sftpa in the endoplasmic reticulum, Sftpb and Sftpc accumulation in the Golgi apparatus, and an increase in the compensatory secretion of Sftpd. Moreover, global and epithelial-specific GM130 knockout in mice resulted in an enlargement of alveolar airspace and an increase in alveolar epithelial autophagy; however, surfactant repletion partially rescued the enlarged airspace defects in GM130-deficient mice. Therefore, our results demonstrate that GM130 and the mammalian Golgi apparatus play a critical role in the control of surfactant protein secretion in pulmonary epithelial cells.

PRMT7 targets of Foxm1 controls alveolar myofibroblast proliferation and differentiation during alveologenesis.

Although aberrant alveolar myofibroblasts (AMYFs) proliferation and differentiation are often associated with abnormal lung development and diseases, such as bronchopulmonary dysplasia (BPD), chronic obstructive pulmonary disease (COPD), and idiopathic pulmonary fibrosis (IPF), epigenetic mechanisms regulating proliferation and differentiation of AMYFs remain poorly understood. Protein arginine methyltransferase 7 (PRMT7) is the only reported type III enzyme responsible for monomethylation of arginine residue on both histone and nonhistone substrates. Here we provide evidence for PRMT7's function in regulating AMYFs proliferation and differentiation during lung alveologenesis. In PRMT7-deficient mice, we found reduced AMYFs proliferation and differentiation, abnormal elastin deposition, and failure of alveolar septum formation. We further shown that oncogene forkhead box M1 (Foxm1) is a direct target of PRMT7 and that PRMT7-catalyzed monomethylation at histone H4 arginine 3 (H4R3me1) directly associate with chromatin of Foxm1 to activate its transcription, and thereby regulate of cell cycle-related genes to inhibit AMYFs proliferation and differentiation. Overexpression of Foxm1 in isolated myofibroblasts (MYFs) significantly rescued PRMT7-deficiency-induced cell proliferation and differentiation defects. Thus, our results reveal a novel epigenetic mechanism through which PRMT7-mediated histone arginine monomethylation activates Foxm1 transcriptional expression to regulate AMYFs proliferation and differentiation during lung alveologenesis and may represent a potential target for intervention in pulmonary diseases.

Large scale RNA-binding proteins/LncRNAs interaction analysis to uncover lncRNA nuclear localization mechanisms.

Long non-coding RNAs (lncRNAs) are key regulators of major biological processes and their functional modes are dictated by their subcellular localization. Relative nuclear enrichment of lncRNAs compared to mRNAs is a prevalent phenomenon but the molecular mechanisms governing their nuclear retention in cells remain largely unknown. Here in this study, we harness the recently released eCLIP data for a large number of RNA-binding proteins (RBPs) in K562 and HepG2 cells and utilize multiple bioinformatics methods to comprehensively survey the roles of RBPs in lncRNA nuclear retention. We identify an array of splicing RBPs that bind to nuclear-enriched lincRNAs (large intergenic non-coding RNAs) thus may act as trans-factors regulating their nuclear retention. Further analyses reveal that these RBPs may bind with distinct core motifs, flanking sequence compositions, or secondary structures to drive lincRNA nuclear retention. Moreover, network analyses uncover potential co-regulatory RBP clusters and the physical interaction between HNRNPU and SAFB2 proteins in K562 cells is further experimentally verified. Altogether, our analyses reveal previously unknown factors and mechanisms that govern lincRNA nuclear localization in cells.

On the conditional performance of the synthetic chart with unknown process parameters using the exceedance probability criterion.

Recent researches on the control charts with unknown process parameters have noticed the large variability in the conditional in-control average run length (ARL) performance of control charts, especially when a small number of Phase I samples is used to estimate the process parameters. Some research works have been conducted on the conditional ARL performance of different types of control charts. In this paper, by simulating the empirical distribution of the conditional ARL and especially using the exceedance probability criterion (EPC), we study the conditional ARL performance of the synthetic [Formula: see text] chart. Our results show that a large amount of Phase I samples is needed to obtain a specified EPC of the synthetic chart. For the available number of Phase I samples, the control limits of the synthetic chart are adjusted using the EPC method to improve its conditional in-control performance. It is shown that, for small mean shift sizes, a tradeoff should be made between the conditional in-control and out-of-control performances. For moderate to large shifts, the conditional performance of the synthetic chart using the adjusted control limits is generally satisfied. By comparing the results with the ones using the bootstrap approach, it can also be concluded that the conditional performances of both approaches are comparable. While the method proposed in this paper requires much less computation work than the bootstrap approach.

Perovskite Sr1-x(Na0.5Bi0.5)xTi0.99Mn0.01O3 Thin Films with Defect Dipoles for High Energy-Storage and Electrocaloric Performance.

Dielectric capacitors have received more and more attention because of fast charge/discharge capability. However, the energy-storage performance still cannot meet the demand. In this work, lead-free perovskite Sr1-x(Na0.5Bi0.5)xTi0.99Mn0.01O3 (x = 0, 0.005, 0.01, and 0.02) thin films prepared by the sol-gel method were carefully studied. Defect dipoles and local lattice distortion were created by doping Mn at the B-site, enabling ferroelectric polarization behavior. To further enhance polarization, co-substitution at the A-site was adopted. Na+ and Bi3+ can make up Na+-Bi3+ ion pairs. Meanwhile, off-center NaSr+ and BiSr3+ ions with a small radius can lead to the distortion of the octahedral [TiO6] in the lattice to induce local polarization regions. Under the combined action of A-site and B-site doping, polarization and breakdown strength were greatly improved. Finally, a high energy density (53 J cm-3) and good thermal stability were achieved. Furthermore, the negative electrocaloric effect was also achieved. The adiabatic temperature change is about -8.5 at 300 K. This work demonstrates that the Sr0.99(Na0.5Bi0.5)0.01(Ti0.99Mn0.01)O3 thin film with excellent energy-storage performance and the negative electrocaloric effect is a promising multifunctional material.

High energy-storage density of lead-free (Sr1-1.5xBix)Ti0.99Mn0.01O3 thin films induced by Bi3+-VSr dipolar defects.

Capacitors with high energy storage density, low cost, ultrafast charge-discharge capability, and environmental friendliness are in high demand for application in new energy vehicles, modern electrical systems, and high-energy laser weapons. Here, lead-free (Sr1-1.5xBix)Ti0.99Mn0.01O3 (x = 0.01, 0.05, 0.1) thin films grown on Pt/Ti/SiO2/Si substrates were obtained by a sol-gel method. All the thin films have a relatively high dielectric breakdown strength (BDS) due to the added 1% Mn and pinched polarization hysteresis loops can be observed in 5 and 10 mol% Bi-doped SrTiO3 thin films. The ferroelectric behaviors of the Bi-doped SrTiO3 thin films come from the rotation of the TiO6-octahedra induced by the formation of Bi3+-VSr dipolar defects. With the increase of doping concentration, the Pmax-Pr values of the Bi-doped SrTiO3 thin films increased dramatically and can reach 34.3 µC cm-2 upon doping with 10 mol% Bi. A high recoverable energy-storage density of 24.4 J cm-3 with excellent temperature stability was obtained for the 10 mol% Bi-doped ST thin film, which shows that the (Sr0.85Bi0.1)Ti0.99Mn0.01O3 thin film is a promising candidate for high-power energy storage applications. This finding demonstrates an improved energy density of SrTiO3-based thin film systems and a reasonable explanation for the source of the ferroelectricity based on first-principles calculations is given.

Measuring the Wave Height Based on Binocular Cameras.

The wave is an important hydrological element in marine research. Accurately describing the characteristics of waves is therefore significant to the study of marine power. The contents of this article are as follows: (1) a wave height measurement system using binocular cameras is proposed, and the small tank experiments are conducted to prove the efficacy of the proposed system; (2) based on the scale invariant feature transition (SIFT) algorithm, sub-pixel Harris corners are calculated in the difference-of-Gaussian (DOG) space to locate key points more accurately; and (3) a bi-directional epipolar constraint is employed to decrease the mismatch rate and computation time.

Thermal Infrared Pedestrian Image Segmentation Using Level Set Method.

The edge-based active contour model has been one of the most influential models in image segmentation, in which the level set method is usually used to minimize the active contour energy function and then find the desired contour. However, for infrared thermal pedestrian images, the traditional level set-based method that utilizes the gradient information as edge indicator function fails to provide the satisfactory boundary of the target. That is due to the poorly defined boundaries and the intensity inhomogeneity. Therefore, we propose a novel level set-based thermal infrared image segmentation method that is able to deal with the above problems. Specifically, we firstly explore the one-bit transform convolution kernel and define a soft mark, from which the target boundary is enhanced. Then we propose a weight function to adaptively adjust the intensity of the infrared image so as to reduce the intensity inhomogeneity. In the level set formulation, those processes can adaptively adjust the edge indicator function, from which the evolving curve will stop at the target boundary. We conduct the experiments on benchmark infrared pedestrian images and compare our introduced method with the state-of-the-art approaches to demonstrate the excellent performance of the proposed method.

Aurora-A promotes the establishment of spindle assembly checkpoint by priming the Haspin-Aurora-B feedback loop in late G2 phase.

Aurora-A kinase functions mainly in centrosome maturation, separation and spindle formation. It has also been found to be amplified or overexpressed in a range of solid tumors, which is linked with tumor progression and poor prognosis. Importantly, Aurora-A inhibitors are being studied in a number of ongoing clinical trials. However, whether and how Aurora-A has a role in the regulation of the mitotic checkpoint is controversial. Additionally, the function of nuclear-accumulated Aurora-A in late G2 phase is not clear. Here we show that knockout, inhibition or blockade of the nuclear entry of Aurora-A severely decreased the centromere localization of Aurora-B and the phosphorylation of histone H3 threonine 3 (H3T3-ph) mediated by the kinase Haspin in late G2 phase. We further reveal that nuclear-accumulated Aurora-A phosphorylates Haspin at multiple sites at its N-terminus and that this promotes H3T3-ph and the rapid recruitment to the centromere of the chromosomal passenger complex. In addition, Aurora-A facilitates the association of Aurora-B with their common substrates: Haspin and Plk1. Notably, these functions of Aurora-A are mostly independent of Plk1. Thus we demonstrate that, in late G2 and prophase, Aurora-A phosphorylates Haspin to trigger the Haspin-H3T3-ph-Aurora-B positive feedback loop that supports the timely establishment of the chromosomal passenger complex and the mitotic checkpoint before spindle assembly.

Defect Engineering of Lead-Free Piezoelectrics with High Piezoelectric Properties and Temperature-Stability.

The high piezoelectricity of ABO3-type lead-free piezoelectric materials can be achieved with the help of either morphotropic phase boundary (MPB) or polymorphic phase transition (PPT). Here, we propose a new defect engineering route to the excellent piezoelectric properties, in which doped smaller acceptor and donor ions substituting bivalent A-sites are utilized to bring local lattice distortion and lower symmetry. A concrete paradigm is presented, (Li-Al) codoped BaTiO3 perovskite, that exhibits a largely thermo-stable piezoelectric constant (>300 pC/N) and huge mechanical quality factor (>2000). A systematic analysis including theoretical analysis and simulation results indicates that the Li(+) and Al(3+) ions are inclined to occupy the neighboring A-sites in the lattice and constitute a defect dipole (ionic pairs). The defect dipoles possess a kind of dipole moment which tends to align directionally after thermo-electric treatment. A mechanism related to the defect symmetry principle, phase transition, and defect migration is proposed to explain the outstanding piezoelectric properties. The present study opens a new development window for excellent piezoelectricity and provides a promising route to the potential utilization of lead-free piezoelectrics in high power applications.