ABSTRACT
BACKGROUND: Dysregulation of long non-coding RNAs (lncRNAs) has been identified in ovarian cancer. However, the expression and biological functions of LINC00852 in ovarian cancer are not understood. METHODS: The expressions of LINC00852, miR-140-3p and AGTR1 mRNA in ovarian cancer tissues and cells were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. Gain- and loss-of-function assays were performed to explore the biological functions of LINC00852 and miR-140-3p in the progression of ovarian cancer in vitro. The bindings between LINC00852 and miR-140-3p were confirmed by luciferase reporter gene assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay. RESULTS: We found that LINC00852 expression was significantly up-regulated in ovarian cancer tissues and cells, whereas miR-140-3p expression was significantly down-regulated in ovarian cancer tissues. Functionally, LINC00852 knockdown inhibited the viability, proliferation and invasion of ovarian cancer cells, and promoted the apoptosis of ovarian cancer cells. Further investigation showed that LINC00852 interacted with miR-140-3p, and miR-140-3p overexpression suppressed the viability, proliferation and invasion of ovarian cancer cells. In addition, miR-140-3p interacted with AGTR1 and negatively regulated its level in ovarian cancer cells. Mechanistically, we found that LINC00852 acted as a ceRNA of miR-140-3p to promote AGTR1 expression and activate MEK/ERK/STAT3 pathway. Finally, LINC00852 knockdown inhibited the growth and invasion ovarian cancer in vivo. CONCLUSION: LINC00852/miR-140-3p/AGTR1 is an important pathway to promote the proliferation and invasion of ovarian cancer.
Subject(s)
Biomarkers, Tumor/metabolism , Gene Expression Regulation, Neoplastic , MicroRNAs/genetics , Ovarian Neoplasms/pathology , RNA, Long Noncoding/genetics , Receptor, Angiotensin, Type 1/metabolism , Animals , Apoptosis , Biomarkers, Tumor/genetics , Cell Proliferation , Female , Humans , Mice , Mice, Inbred BALB C , Mice, Nude , Ovarian Neoplasms/genetics , Ovarian Neoplasms/metabolism , Prognosis , Receptor, Angiotensin, Type 1/genetics , Tumor Cells, Cultured , Xenograft Model Antitumor AssaysABSTRACT
In this study, we determine the electron tomography (ET) resolution for microns-thick specimens by experiment in the ultra-high voltage electron microscope. A tilt series of projection images of a tilted 8µm thick epoxy-resin film are first acquired. Tomographic reconstructions are then calculated and the resolution is evaluated with the Fourier shell correlation method. The ET resolution of 32nm is achieved under the condition of 2MV accelerating voltage. We also demonstrate that some high tilt angle projections may be little useful for improving the final ET resolution because of the corresponding poor image qualities. These results are helpful to understand the possibility and limitation of ET applications in microns-thick specimens.
ABSTRACT
Several strains of phosphate-solubilizing bacteria were isolated and screened from the crop rhizosphere of calcareous soil in Shanxi Province of China. After repeated isolation and purification, the strain W25 with strong phosphate-solubilizing activity was obtained, and identified as Rahnella sp., based on the morphological, physiological and biochemical properties and the analysis of 16S rRNA gene sequence. Further studies on the W25 showed that the maximum phosphate-solubilizing capability of the W25 on tricalium phosphate, aluminum phosphate and ferric phosphate reached 385.5, 110.4 and 216.6 mg x L(-1), respectively. In the liquid culture with aluminum phosphate and ferric phosphate, the solubilized phosphorous by the W25 was significantly negatively correlated with the liquid pH, with the correlation coefficient being 0.56 and 0.81, respectively. Among the carbon and nitrogen sources, glucose and ammonium nitrate were the optimum for the solubilization of tricalium phosphate by W25. The utilization of carbon source was in the order of glucose > lactose > sucrose > mannitose > starch, and that of nitrogen source was in the order of ammonium nitrate > ammonium chloride > ammonium sulfate > potassium nitrate > sodium nitrate. Different nitrogen sources had greater effects on the production of organic acids by W25. Formic acid and acetic acid would be produced when the nitrogen source was NH4+, oxalic acid and succinic acid would be produced when the nitrogen source was NO3(-), and citric acid would be extra produced when the ammonium nitrate was used as the nitrogen source.
