The combined contamination of nano-polystyrene and nanoAg: Uptake, translocation and ecotoxicity effects on willow saplings.

Nanoplastics (NPLs) and nanoAg (AgNPs) are emerging contaminants commonly detected in aquatic and terrestrial environments due to their widespread use in various domains. However, their uptake, translocation, and toxic effects on plants in cooccurrence environments remain largely unexplored. Therefore, a hydroponic experiment was conducted using 100 nm NPLs (1 mg/L and 10 mg/L), AgNPs (100 µg/L and 1000 µg/L) and saplings of willow (Salix matsudana 'J172') to investigate absorption, translocation and the physio-biochemical responses of the plants. The results indicated that NPLs and AgNPs were agglomerated with each other in solutions. NPLs not only penetrated the roots of the saplings but also translocated to the branches and leaves through xylem ducts. However, AgNPs was only detected in the roots, suggesting that the internalization of nanoparticles in plants depends on the properties and types of particles themselves. The combined exposure to NPLs and AgNPs selectively affected the absorption and distribution of K, Ca, Mg and Fe, resulting in inhibited saplings growth and photosynthesis. Furthermore, the presence of NPLs and AgNPs induced oxidative damage and stimulated the antioxidant stress system in the plants. This study provides novel insights into the internalization and ecotoxicological mechanisms of NPLs and AgNPs in woody vascular plants.

Metal tolerance capacity and antioxidant responses of new Salix spp. clones in a combined Cd-Pb polluted system.

To investigate the physiochemical characteristics of two new clones, Salix matsudana 'J172' (A7) and Salix matsudana 'Yankang1' (A64) in combined Cd-Pb contaminated systems, a hydroponic experiment was designed. The plant biomass, photosynthesis, antioxidant responses and the accumulation of metals in different plant parts (leaf, stem, and root) were measured after 35-day treatments with Cd (15, 30 µM) and Pb (250, 500 µM). The results showed that exposure to Cd-Pb decreased the biomass but increased the net photosynthetic rate for both A7 and A64, demonstrating that photosynthesis may be one of the metabolic processes used to resist Cd-Pb stress. Compared with control, roots exposed to Cd-Pb had higher activity of superoxide dismutase and more malondialdehyde concentrations, which indicated the roots of both clones were apt to be damaged. The concentrations of soluble protein were obviously higher in the roots of A64 than A7, indicating the roles of the antioxidative substance were different between two willow clones. Soluble protein also had significant relationship with translocation factors from accumulation in roots of A64, which illustrated it played important roles in the tolerance of A64 roots to heavy metals. The roots could accumulate more Pb rather than transport to the shoots compared with Cd. The tolerance index was more than 85% on average for both clones under all the treatments, indicating their tolerance capacities to the combined stress of Cd and Pb are strong under the tested metal levels. Both clones are the good candidates for phytoremediation of Cd and Pb by the root filtration in the combined contamination environment.

Coupling antecedent rainfall for improving the performance of rainfall thresholds for suspended sediment simulation of semiarid catchments.

Suspended sediment transport is one of the essential processes in the geochemical cycle. This study investigated the role of rainfall thresholds in suspended sediment modeling in semiarid catchments. The results showed that rainfall-sediment in the study catchment (HMTC) could be grouped into two patterns on the basis of rainfall threshold 10 mm. The sediment modeling based on LSTM model with the rainfall threshold (C-LSTM scheme) and without threshold (LSTM scheme) were evaluated and compared. The results showed that the C-LSTM scheme had much better performances than LSTM scheme, especially for the low sediment conditions. It was observed that in the study catchment, the mean NSE was marginally improved from 0.925 to 0.934 for calibration and 0.911 to 0.924 for validation for medium and high sediment (Pattern 1); while for low sediment (Pattern 2), the mean NSE was significantly improved from -0.375 to 0.738 for calibration and 0.171 to 0.797 for validation. Results of this study indicated rainfall thresholds were very effective in improving suspended sediment simulation. It was suggested that the incorporation of more information such as rainfall intensity, land use, and land cover may lead to further improvement of sediment prediction in the future.

Ectomycorrhizal fungi enhance the tolerance of phytotoxicity and cadmium accumulation in oak (Quercus acutissima Carruth.) seedlings: modulation of growth properties and the antioxidant defense responses.

Ectomycorrhizal fungi (EMF), which form symbiotic ectomycorrhiza with tree roots, mediate heavy metal tolerance of host plants. To investigate the roles of EMF in the growth, modulation of oxidative stress, and cadmium (Cd) accumulation and translocation in Quercus acutissima seedlings, ectomycorrhizal seedlings inoculated with Suillus luteus were treated with different Cd concentrations (0.1, and 5 mg kg-1) for 14 days. EMF accelerated seedling growth and Cd accumulation in roots under the highest Cd concentration of 5 mg kg-1. Catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) activities increased in the leaves of ectomycorrhizal seedlings under the highest Cd concentration. Superoxide dismutase (SOD) trended to increase under both Cd concentrations. Although reduced glutathione (GSH) increased after inoculation of EMF under both Cd concentrations, the release of malondialdehyde increased in the leaves and roots under the highest Cd concentration, indicating that the defense role of EMF in Q. acutissima depends on the Cd concentration. These results indicate that EMF mitigate Cd stress by promoting plant growth and nutrient uptake while modulating the antioxidant system to reduce oxidative stress.

Effects of Vegetation Restoration on Soil Enzyme Activity in Copper and Coal Mining Areas.

Mining areas are suffering from serious environmental hazards, such as soil erosion, water pollution as well as land degradation. In this study, two types of mining areas in Anhui Province, China-one a copper mining area and the other a coal mining area-were selected to compare the soil properties under different vegetation restoration conditions, which can be generally classified into reclaimed and non-reclaimed areas. Soil catalase and urease activities and soil chemical properties were chosen to be the main indicators of soil quality. Principal component analysis was used to evaluate the overall soil fertility in the copper and coal mining areas. Results showed that in the copper mining area soil catalase activity was between 12.36 and 19.17 µg g-1 h-1 and urease activity was between 0.03 and 12.05 µg g-1 h-1. And in coal mining area, soil catalase activity was between 3.52 and 9.72 µg g-1 h-1 and urease activity was between 2.71 and 10.81 µg g-1 h-1. Moreover, soil catalase and urease activities in degraded areas were lower than those in reclaimed areas. Soil catalase activity and soil urease activity were significantly correlated with total potassium and total nitrogen, respectively. Soil quality in land types with vegetation restoration was higher than in non-reclaimed areas and old subsidence areas, while soil quality in the copper mining area was generally higher than in the coal mining area. Thus, the optimum measure in this region to ameliorate these degraded soils is vegetation restoration, which helps not only to improve the environment, but also to enhance soil quality in these degraded lands.

Circular RNA hsa_circ_0032463 Acts as the Tumor Promoter in Osteosarcoma by Regulating the MicroRNA 498/LEF1 Axis.

Several studies have examined the relationship between osteosarcoma (OS) and microRNAs (miRNAs). However, only a few researchers have investigated the underlying mechanism of circular RNAs (circRNAs) in OS development. Our paper aimed to assess how hsa_circ_0032463 (abbreviated "circ_0032463" here) initiates and regulates OS progression. We detected circ_0032463 expression in OS tissues and cell lines by using reverse transcription-quantitative PCR (RT-qPCR) analysis and then investigated the interaction between circ_0032463, miRNA 489 (miR-498), and LEF1 using RNA pulldown, RNA immunoprecipitation (RIP), and luciferase assays. The effect of the circ_0032463/miR-498/LEF1 axis on the migration, proliferation, and apoptosis levels of OS cells was explored using CCK-8, bromodeoxyuridine (BrdU), wound healing, and fluorescein isothiocyanate (FITC) assays. Our findings revealed that circ_0032463 expression was upregulated in OS tissues and cell lines. We also found that circ_0032463 interacted with miR-498, thereby reducing the expression of miR-498 in OS cells. Experimental results indicated that miR-498 could directly target LEF1 in OS cells and that circ_0032463 could abrogate the tumor-inhibitory effect of miR-498 by upregulating LEF1 in OS. More specifically, by binding to miR-498 and inhibiting LEF1 expression, circ_0032463 promoted the migration and proliferation abilities of OS cells and suppressed the apoptosis ability of OS cells. Overall, this research suggested that circ_0032463 could promote OS development by regulating the miR-498/LEF1 axis.

Hsa_circ_0032463 acts as the tumor promoter in osteosarcoma by regulating the miR­330­3p/PNN axis.

Osteosarcoma (OS), also known as bone cancer, is a threat to the lives of millions of adolescents worldwide. Although dedicated efforts have been invested in reducing the mortality rate of this bone cancer, the research community is yet to find the exact causes of OS. Thus, the present research aimed to study the association between circular RNA circ_0032463 and OS progression. The impact of circ_0032463 on cells with OS was first evaluated using reverse transcription­quantitative PCR. This evaluation was followed by the assessment of cell proliferation, viability, apoptosis, invasion and adhesion using BrdU, Cell Counting Kit­8, flow cytometry, Transwell and cell adhesion assays, respectively. RNA pull­down, RNA immunoprecipitation chip and dual­luciferase reporter systems were utilized to investigate the relationship between circ_0032463, microRNA (miR)­330­3p and Pinin desmosome associated protein (PNN) in OS. The findings indicated that circ_0032463 and PNN were highly expressed in OS tissues and OS cell lines, and that they facilitated cell proliferation, viability, invasion and adhesion, but attenuated cell apoptosis in OS cells. The low expression of miR­330­3p suppressed OS development. It was also noted that circ_0032463 inhibited miR­330­3p to upregulate PNN expression. In conclusion, this study confirmed that by regulating the miR­330­3p/PNN axis, circular RNA circ_0032463 could function as a tumor enhancer in cells with OS.

Effect of different vegetation on copper accumulation of copper-mine abandoned land in tongling, China.

Mining activity and abandoned mine land are one of the major sources of heavy metal pollution. Thus, ecological rehabilitation of abandoned mine lands is crucial to control heavy metal pollution. This research aims to explore the influencing factors and effects of different vegetation on copper (Cu) accumulation and soil amelioration. In this study, the abandoned land of Tongguanshan Cu mine in Tongling city, Anhui province, China, was chosen as the test area, and nine sampling points were established. Samples of soil and plants were collected from each plot, and the impacts of Cu pollution on soil enzymes and other features were analyzed, as well as the correlation between Cu accumulation of different plants and soil properties. The results showed that Cu content of soil in the Tongguanshan area varied greatly with the depth of the soil profile. Moreover, Cu in the soil can inhibit soil enzyme activities; and the correlation coefficients of total soil Cu with urease and catalase were -0.83 and -0.73, respectively. Clearly, the accumulation of Cu in plants was positively correlated with Cu content in soil. It was found that Pueraria lobata had the best remediation effect on soil Cu pollution in a short period of time. Hence the preliminary tests clearly indicate that phytoremediation in abandoned mine lands can not only reduce heavy metal pollution, but also enhance soil nutrition and enzyme activity, helping to ameliorate degraded land and promote regional socioeconomic sustainable development.

Growth, accumulation, and antioxidative responses of two Salix genotypes exposed to cadmium and lead in hydroponic culture.

Cd and Pb are a toxic environmental pollutant, and their elevated concentrations in the waters and soils could exert detriment effects on human health by food chain. In order to evaluate the capacity to heavy metal accumulation and the physiochemical responses of two Salix genotypes, a 35-day hydroponic seedling experiment was implemented with Salix matsudana Koidz. 'Shidi1' (A42) and Salix psammophila C. 'Huangpi1' (A94) under different concentrations of Cd (15 and 30 µM) or Pb (250 and 300 µM). The results showed that the biomass of A94 severely reduced more than that of A42. The accumulation ability of Cd in different plant organs followed the sequence of leaves > roots > stems. Pb primarily accumulated in the roots for both Salix genotypes (54.27 mg g-1 for A42 and 54.52 mg g-1 for A94). Translocation factors based on accumulation (TF') for Cd were more than 8.0, while TF's for Pb were less than 1.0 in both A42 and A94, implying they could be applied in the phytoremediation of Cd-contaminated sites due to their stronger ability to Cd phytoextraction. The stress of Cd or Pb significantly increased malondialdehyde (MDA) contents and increased photosynthetic rates in leaves of two Salix genotypes. Transpiration rates of willow were positively correlated with its Cd translocation. Both catalase (CAT) and peroxidase (POD) activities were suppressed, while the superoxide dismutase (SOD) was boosted with increasing Cd and Pb levels in the leaves and roots of the two willow genotypes, suggesting SOD plays an important role in the removal of ROS. The inconsistency of the changes in enzyme activity suggests that the integrated antioxidative mechanisms regulate the tolerance to Cd and Pb stress.

Response of Soil Microbes to Vegetation Restoration in Coal Mining Subsidence Areas at Huaibei Coal Mine, China.

Vegetation restoration is an available way to ameliorate degraded lands. In order to study the response of soil microbes to vegetation restoration in coal mining subsidence areas, the composition and distribution of soil microbes were discussed through three plots: unsubsided area (CA), new subsided area (NSA), and old subsided area (OSA) with different vegetation restoration time in Huabei coal mine. Meanwhile, changes in soil catalase and urease activity were explored and the correlation between soil bacteria, fungi, and environmental factors was analysed. The results demonstrated that Nitrospira was the dominant bacteria in all areas sampled. Microorganisms in the 0-20 cm and 40-60 cm soil layers of OSA had the highest Simpson index, whereas the index in NSA was lowest (at all soil depths). The catalase activity in NSA was significantly higher than that in CA, and there was no significant difference in catalase activity with soil depth, while the urease activity declined gradually with increasing soil depth. The urease activity in the 20-60 cm soil layer of NSA and OSA was significantly higher than that of CA. Furthermore, the distribution of bacteria was mainly affected by soil organic matter, available potassium, available phosphorus, and alkali-hydrolyzable nitrogen, whereas pH and catalase activity mainly affected fungal distribution. These results implied that soil catalase activity in NSA and urease activity in the 20-40 cm soil layer of NSA and OSA were significantly enhanced after vegetation restoration, and that long-term plant restoration could improve soil fertility and soil microbial community diversity in coal mining areas.

Genome-wide analysis of SSR and ILP markers in trees: diversity profiling, alternate distribution, and applications in duplication.

Molecular markers are efficient tools for breeding and genetic studies. However, despite their ecological and economic importance, their development and application have long been hampered. In this study, we identified 524,170 simple sequence repeat (SSR), 267,636 intron length polymorphism (ILP), and 11,872 potential intron polymorphism (PIP) markers from 16 tree species based on recently available genome sequences. Larger motifs, including hexamers and heptamers, accounted for most of the seven different types of SSR loci. Within these loci, A/T bases comprised a significantly larger proportion of sequence than G/C. SSR and ILP markers exhibited an alternative distribution pattern. Most SSRs were monomorphic markers, and the proportions of polymorphic markers were positively correlated with genome size. By verifying with all 16 tree species, 54 SSR, 418 ILP, and four PIP universal markers were obtained, and their efficiency was examined by PCR. A combination of five SSR and six ILP markers were used for the phylogenetic analysis of 30 willow samples, revealing a positive correlation between genetic diversity and geographic distance. We also found that SSRs can be used as tools for duplication analysis. Our findings provide important foundations for the development of breeding and genetic studies in tree species.

Contrasting runoff trends between dry and wet parts of eastern Tibetan Plateau.

As the "Asian Water Tower", the Tibetan Plateau (TP) provides water resources for more than 1.4 billion people, but suffers from climatic and environmental changes, followed by the changes in water balance components. We used state-of-the-art satellite-based products to estimate spatial and temporal variations and trends in annual precipitation, evapotranspiration and total water storage change across eastern TP, which were then used to reconstruct an annual runoff variability series for 2003-2014. The basin-scale reconstructed streamflow variability matched well with gauge observations for five large rivers. Annual runoff increased strongly in dry part because of increases in precipitation, but decreased in wet part because of decreases in precipitation, aggravated by noticeable increases in evapotranspiration in the north of wet part. Although precipitation primarily governed temporal-spatial pattern of runoff, total water storage change contributed greatly to runoff variation in regions with wide-spread permanent snow/ice or permafrost. Our study indicates that the contrasting runoff trends between the dry and wet parts of eastern TP requires a change in water security strategy, and attention should be paid to the negative water resources impacts detected for southwestern part which has undergone vast glacier retreat and decreasing precipitation.

Curcumin ameliorates the permeability of the blood-brain barrier during hypoxia by upregulating heme oxygenase-1 expression in brain microvascular endothelial cells.

Curcumin (Cur) is a major active component of the food flavor turmeric isolated from the powdered dry rhizome of Curcuma longa Linn., which has been used in traditional Chinese medicine to ameliorate intracerebral ischemic damage and reduce brain edema. However, the effects of Cur on the disruption of the blood-brain barrier (BBB) induced by brain ischemia are still unclear. The effects of Cur on the disruption of BBB and changes of tight junction (TJ) proteins induced by oxygen glucose deprivation (OGD) were studied in BBB in vitro. The transendothelial electrical resistance and the flux of horseradish peroxidase in BBB in vitro were measured. The expression and localization of the TJ proteins occludin and zonula occludens-1 (ZO-1) were evaluated by Western blots and immunofluorescence microscopy. The protein levels of heme oxygenase-1 (HO-1) were also analyzed via Western blots. Cur attenuated OGD-induced disruption of paracellular permeability and increased the expression of HO-1 protein in rat brain microvascular endothelial cells (RBMECs). After administration of OGD, the expression of occludin and ZO-1 proteins was restored by Cur, and this effect was blocked by a HO-1 inhibitor, zinc protoporphyrin (ZnPP). Cur protects RBMECs against OGD-induced disruption of TJ and barrier dysfunction via the HO-1 pathway. We propose that Cur is capable of improving the barrier function of BBB under ischemic conditions and this beneficial effect might be reversed by a HO-1 inhibitor, ZnPP.