The Construction of a Diversified and Open Teacher Education System Management System That Integrates Public Mental Health.

The teacher education management system is crucial to the operation of the teacher education system (TES). It is the core institutional system of the TES, which stipulates the management authority and functional scope of the relevant departments of the TES. After the new pluralistic and open TES is formed, the original management system must be adjusted accordingly. In this context, this article integrates public mental health (PMH) into the diversified and open management system of TES and then uses a neural network to evaluate and complete the following tasks: (1) this article introduces the development status of diversified and open TES management systems at home and abroad and the importance of public psychology courses to teacher education. (2) The framework of TES integrating PMH is proposed, and then the principles of BPNN and gray wolf optimization (GWO) algorithm are introduced, and the IGWO-BPNN model is constructed accordingly. (3) The convergence and fitness of the IGWO-BP model and the GWO-BP model are compared through experiments, which proves the superiority of the performance of the IGWO-BP model. The optimal model is constructed by selecting parameters, and then the output of the model is compared with the expert evaluation results, and the error is small. It is proved that the model has superior performance in evaluating the professional quality of teacher education. (4) This article selects three professional quality evaluation indicators of teacher education to compare the changes before and after the integration of PMH. The results show that the proposed diversified and open management system of TES integrating PMH can effectively improve the professional quality of teachers in all aspects.

Elucidation of the mechanism of miR-122-5p in mediating FOXO3 injury and apoptosis of mouse cochlear hair cells induced by hydrogen peroxide.

Unveiling the mechanism of miR-122-5p in the mediation of forkhead box O3 (FOXO3) in regards to cochlear hair cell damage provides an effective solution for the treatment of ear hearing disorders. An oxidative stress model using a mouse cochlear hair cell line (HEI-OC1) was established via hydrogen peroxide (H2O2). Then HEI-OC1 cells were transfected with miR-122-5p mimic, miR-122-5p inhibitor, and lentiviral vector FOXO3-WT/MUT. Cell viability and apoptosis rate were determined by MTT assay and flow cytometry. Reactive oxygen species (ROS) were observed by confocal laser scanning microscopy. Bcl-2, Bax, capase-3 and c-caspase-9 levels were quantified by western blot analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was used to detect superoxide dismutase (SOD) and malondialdehyde (MDA) levels, and flow cytometry was performed to measure the mitochondrial membrane potential levels. In the HEI-OC1 oxidative stress model after transfection, the miR-122-5p level was decreased, whereas the FOXO3 level was increased, Moreover, the increased FOXO3 level diminished the cell viability, but promoted cell apoptosis. Apart from this, the Bcl-2 level was downregulated, while levels of Bax, c-caspase-3, c-caspase-9, ROS and MDA were upregulated. Meanwhile, the mitochondrial membrane potential level was also elevated. Overexpression of miR-122-5p was able to partially offset the effects of FOXO3 in the H2O2-treated HEI-OC1 cells. Collectively, miR-122-5p restrained the decrease in HEI-OC1 cell viability and apoptosis induced by treatment with H2O2.

[The effect of sodium salicylate on the expression of GABAa receptor subunits in cochlear spiral ganglion neurons].

OBJECTIVE: To investigate mRNA expression of GABAa receptor(GABAaR) subunits in the rat cochlear spiral ganglion neurons (SGN) and explore the effect of sodium salicylate (SS) on the expression of GABAaR subunits. METHOD: The realtime fluorescent quantitative PCR (FQ-PCR) was used to detect mRNA expression of twelve GABAaR subunits in the newborn rat SGN and then investigate mRNA expression of GABAaR subunits after treatment with 5 mmol/L SS for 15 min, 30 min, 1 h, 3 h and 6 h in the primary culture SGN. RESULT: (1) GABAaR subunits of α1-6, ß1-3, and γ1-3 were detected in the SGN, and the expression of GABAaR subunits was lower than those in the cerebral cortex. In the subunit α family of GABAaR, the expression rank was α2>α3/α5>α4>a1>α6, and the expression of α3 and α5 had no difference (P>0. 05). In the subunit ß family, the expression rank was ß3>ß2>ß1. In the subunit γ family, the expression rank was γ1>γ2>γ3. (2) The expression of all subunits of GABAa receptor was obviously fluctuated excepting subunit α5 after treatment with SS. At 15 min post-SS, α1, α2 , ß1 and γ1-3 were upregulated, and α3 was downregulated; At 30 min post-SS, α3, ß1 and ß3 were upregulated, and γ1 was downregulated; At 1 h post-SS, ß2 was upregulated and γ3 was downregulated; At 3 h post-SS, ß1 and ß2 were upregulated, and α3 and γ2 were downregulated; At 6 h post-SS, αl, α3 ,ß2, ß3 and γ1 were upregulated, and α2, α4 and ß1 were downregulated. CONCLUSION: The mRNA of GABAaR was expressed in the rat SGN, and the expression of GABAaR subunits was lower in SGN than the cerebral cortex. SS could alter the GABAaR expression quantity in rat SGN; Most of the subunits expression were elevated obviously in the early post SS (15 min), followed by a slight fluctuation.

[siRNA-mediated CDK6 knockdown suppresses nasopharyngeal carcinoma cell growth and cell cycle transition in vitro].

OBJECTIVE: To assess the effect of small interfering RNA (siRNA)-mediated suppression of CDK6 expression on the proliferation and cell cycles of nasopharyngeal carcinoma (NPC) cells in vitro. METHODS: QRT-PCR was used to examine the differential expression of CDK6 in 30 NPC tissues and 18 normal nasopharyngeal tissues. A siRNA targeting CDK6 was transfected in NPC CNE2 cells, and MTT assay and flow cytometry were used to analyze the changes in cell proliferation and cell cycle distribution. Western blotting was used to examine the expressions of the cell cycle-related factors. RESULTS: Compared with normal nasopharyngeal tissues, NPC tissues showed an increased expression of CDK6 mRNA. Knocking down CDK6 expression obviously inhibited tumor cell growth and cell cycle transition from G1 to S phase and caused reduced expressions of CDK4, CCND1, and E2F1 and enhanced expression of the tumor suppressor p21. CONCLUSION: NPC tissues overexpress CDK6. Knocking down CDK6 expression inhibits the growth and cell cycle transition of NPC cells in vitro by inhibiting the expressions of CDK4, CCND1, and E2F1 and upregulating tumor suppressor p21 expression.

[Expression of CDK6 in nasopharyngeal carcinoma and its clinical significance].

OBJECTIVE: To explore the expression of CDK6 in nasopharyngeal carcinoma (NPC) and explore its clinical significance. METHODS: Immunohistochemistry was used to examine the differential expression of CDK6 protein in 101 NPC and 30 nasopharyngeal tissues, and the correlation of CDK6 expression with the clinical characteristics was analyzed in NPC cases. RESULTS: Immunohistochemistry demonstrated that CDK6 protein was a co-expressed factor in the cytoplasm and cell nuclei. CDK6 was expressed predominantly in the cytoplasm in nasopharyngeal tissues, but in NPC tissues, CDK6 was co-expressed predominantly in the cytoplasm and nuclei. Compared to the nasopharyngeal tissues, NPC tissues showed significantly up-regulated CDK6 expression (P=0.009) in positive correlation with tumor size (P=0.020) and clinical stages (P=0.0039). CONCLUSIONS: Increased CDK6 protein expression is an unfavorable factor that promotes the development and progression of NPC.

[A case report of nasal myiasis].

A 66-year-old male patient presented with rhinocnesmus and mild epistaxis. More than 100 maggots were found in the right nasal cavity by nasal endoscopy. The affected nasal mucosa was erythematous, edematous, ulcerated, and mild bleeding on probing was present. No nasal septal perforation or destruction of nose was noted. Middle and inferior meatus, nasopharyngeal mucosa, orbit, facial skin, oral cavity, gingiva and external auditory canal were normal. The maggot was identified as larvae of Chrysomyia megacephala.