ABSTRACT
BACKGROUND: Wilms tumor (WT) is the most common pediatric embryonal tumor. Improving patient outcomes requires advances in understanding and targeting the multiple genes and cellular control pathways, but its pathogenesis is currently not well-researched. We aimed to identify the potential molecular biological mechanism of WT and develop new prognostic markers and molecular targets by comparing gene expression profiles of Wilms tumors and fetal normal kidneys. METHODS: Differential gene expression analysis was performed on Wilms tumor transcriptomic data from the GEO and TARGET databases. For biological functional analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were utilized. Out of 24 hub genes identified, nine were found to be prognostic-related through univariate Cox regression analysis. These nine genes underwent LASSO regression analysis to enhance the predictive capability of the model. The key hub genes were validated in the GSE73209 datasets, and cell function experiments were conducted to identify the genes' functions in WiT-49 cells. RESULTS: The enrichment analysis revealed that DEGs were significantly involved in the regulation of angiogenesis and regulation of cell differentiation. 24 DEGs were identified through PPI networks and the MCODE algorithm, and 9 of 24 genes were related to WT patients' prognosis. EMCN and CCNA1 were identified as key hub genes, and related to the progression of WT. Functionally, over-expression of EMCN and CCNA1 knockdown inhibited cell viability, proliferation, migration, and invasion of Wilms tumor cells. CONCLUSIONS: EMCN and CCNA1 were identified as key prognostic markers in Wilms tumor, suggesting their potential as therapeutic targets. Differential gene expression and enrichment analyses indicate significant roles in angiogenesis and cell differentiation.
Subject(s)
Biomarkers, Tumor , Computational Biology , Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Kidney Neoplasms , Wilms Tumor , Wilms Tumor/genetics , Wilms Tumor/pathology , Humans , Computational Biology/methods , Kidney Neoplasms/genetics , Kidney Neoplasms/pathology , Biomarkers, Tumor/genetics , Prognosis , Gene Regulatory Networks , Transcriptome , Cell Proliferation/genetics , Protein Interaction Maps/genetics , Gene Ontology , Cell Line, TumorABSTRACT
To improve corrosion performance of coatings on AZ31B magnesium alloy, the nano-CeO² additives were included in Na²SiO³ based electrolyte during process of pulse micro arc oxidation (MAO). The MAO-CeO² coating was successfully prepared to characterize its structure, micro morphology and composition. The XRD results indicated that MAO-CeO² coatings were consisted of Mg²SiO4, MgSiO³, MgF² and CeO². The intensity of CeO² peaks increases with increasing nano-CeO² particles in electrolyte. The number of cracks on MAO-CeO² coatings exhibited a V-shaped trend with increase in nano-CeO² content. Meanwhile, the influence of nano-CeO² on corrosion behavior of MAO-CeO² coatings is investigated by salt spray test and electrochemical measurement. The corrosion current density of coatings presented same trend and corrosion potential is further studied. The MAO-CeO² coatings formed in electrolyte with 10 g/L nano-CeO² showed best corrosion performance which has the lowest corrosion current density of 0.58 nA/cm² and the highest corrosion potential of - 1269 mVSCE.
ABSTRACT
A duplex CeO2/MAO coating was deposited on AZ31B magnesium alloy through microarc oxidation and reactive radio frequency magnetron sputtering. The microstructure and chemical composition of the proposed coating were investigated through scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The CeO2/MAO coating was approximately 580 nm in thickness and showed fewer micropores and defects than those the MAO coating presented. The properties of corrosion resistance were assessed using the polarization test, electrochemical impedance spectroscopy, and the neutral salt spray test. The CeO2/MAO duplex coating had the lowest corrosion current density of 3.25×10-9 A/cm² and the highest polar resistance, approximately four orders of magnitude higher than that of the uncoated sample. Electrochemical measurements suggested that CeO2 coating can serve as a strong physical barrier between the corrosion medium and the Mg alloy.
