ABSTRACT
Objective:To investigate the molecular mechanism of Qiyu Sanlong prescription （QYSL） in inhibiting the "addiction" of lung cancer A549 cells to miRNA21. Method:The human lung cancer A549 cells were routinely passaged and divided into the blank group， blank serum group， QYSL-containing serum group， and siRNA group. The prepared QYSL-containing serum was used for intervention， with the optimal concentration and action time determined in previous studies. The protein and mRNA expression levels of miRNA21 and related molecules in its target phosphatase and tensin homolog deleted on chromosome 10 （PTEN）/phosphatidylinositol 3-kinase （PI3K） signaling pathway were detected by real-time polymerase chain reaction （Real-time PCR） and Western blot assay. Result:The comparison with the blank serum group revealed that the mRNA expression levels of miRNA21 in the QYSL-containing serum group and the siRNA group were decreased， while the PTEN mRNA expression in the QYSL-containing serum group was increased， showing significant differences （<italic>P</italic><0.01）. Compared with the blank serum， the QYSL-containing serum and siRNA significantly down-regulated PI3K and mammalian target of rapamycin （mTOR） mRNA expression （<italic>P</italic><0.01）， whereas the QYSL-containing serum did not change the mRNA expression of protein kinase B （Akt）. The protein expression levels of PTEN in the QYSL-containing serum group and the siRNA group were obviously elevated in contrast to that in the blank serum group （<italic>P</italic><0.05）. Meanwhile， the protein expression levels of phosphorylated Akt （p-Akt） and phosphorylated mTOR （p-mTOR） evidently declined in the QYSL-containing serum group （<italic>P</italic><0.05）， but there was no significant reduction in total Akt and mTOR protein expression. The PI3K protein expression was slightly down-regulated， with no statistical significance. Conclusion:QYSL inhibits the transcription of miRNA21， increases the expression of PTEN， and reduces the expression of key molecules in PI3K/Akt/mTOR signaling pathway， thus mildly inhibiting the "addiction" of lung cancer cells to oncogenes and blocking their proliferation.
ABSTRACT
Objective:To evaluate the effect of Mashao Pingchuan decoction on traditional Chinese medicine <bold>（</bold>TCM<bold>）</bold> symptoms， quality of life， peripheral blood eosinophils （Eos） and serum inflammatory factors interleukin-6 （IL-6） and interleukin-8 （IL-8） in patients with cold asthma syndrome of bronchial asthma. Method:A total of 67 patients with cold asthma who attended the Respiratory Clinic of Anhui Provincial Hospital of TCM from January 2018 to December 2019 were selected and randomly divided into a control group and an observation group. The control group was given basic treatments such as budesonide formoterol powder inhalation， and the observation group was given Mashao Pingchuan decoction on the basis of the control group. Both groups intervened for 7 consecutive days. Observe and record the general condition， TCM symptom score， asthma control questionnaire （ACQ）-7 score， Marks-asthma quality of life questionnaire （Marks-AQLQ） score of the two groups of asthma patients， the peripheral blood Eos count was measured by hematology analyzer， and the serum IL-6 and IL-8 levels of the subjects were measured by enzyme-linked immunosorbent assay （ELISA）. Result:The total effective rate of TCM symptoms in the two groups was 100%（30/30）， and the effect in the observation group was more obvious （<italic>Z</italic>=-2.169，<italic>P</italic><0.05<italic>）</italic>. After treatment， the scores of TCM symptoms in the two groups were significantly lower than those before treatment （<italic>P</italic><0.01）. The scores of phlegm in the throat， expectoration， cough， and chills in the observation group were lower than those in the control group （<italic>P</italic><0.05）. After treatment， the ACQ-7 scores and Marks-AQLQ scores of the two groups were significantly lower than before treatment （<italic>P</italic><0.01）. There was no statistically significant difference between the observation group and the ACQ-7 scores and Marks-AQLQ scores. After treatment， the peripheral blood Eos counts and serum IL-6 and IL-8 levels in the two groups were significantly lower than before treatment （<italic>P</italic><0.01）. Peripheral blood Eos count and serum IL-6 and IL-8 contents in observation group were lower than those in control group （<italic>P</italic><0.05）. Conclusion:Mashao Pingchuan decoction combined with budesonide formoterol powder inhalation can effectively improve the clinical effectiveness of asthma （cold asthma）， improve the symptoms of asthma in TCM， ACQ-7 score， Marks-AQLQ score， peripheral blood Eos count， serum inflammatory factor content.
ABSTRACT
Objective:To summarize medication law of prescription and clinical experience on the treatment of chronic cough through data mining technology. Method:The formula information of 650 cases of chronic cough cases diagnosed and treated by professor HAN Ming-xiang was processed with data mining technology, and system cluster analysis and factor analysis were conducted by SPSS Statistics 22.0. Result:High-frequency medicines of professor HAN Ming-xiang for treating chronic cough were Stemonae Radix, Citri Reticulatae Pericarpium, Asteris Radix et Rhizoma and other 37 herbs, their frequency of occurrence accounted for 80.52%. According to the efficacy, the medicines could be classified into 10 categories, such as expectorant cough antiasthmatic drugs, tonifying deficiency drugs, and diaphoretic drugs. Cluster analysis showed 5 combinations of drugs. Factor analysis yielded 13 common factors, such as Stemonae Radix-Asteris Radix et Rhizoma-Farfarae Flos-Cynanchi Stauntonii Rhizoma et Radix, Asari Radix et Rhizoma-Schisandrae Chinensis Fructus-Zingiberis Rhizoma-Fritillariae Thunbergii Bulbus. Conclusion:Combined with clinical experience, it can be concluded that clinical treatment of professor HAN Ming-xiang for chronic cough attaches great importance to the pathogenesis of wind-evil, Yang deficiency and cold phlegm underlying lung, phlegm and blood stasis and Qi stagnation. The treatment mainly starts from the lung and spleen, with the method of warm and moist, or with some traditional Chinese medicines with the function of removing phlegm by warming the lung, dispersing cold by thinning the surface, strengthening the spleen and resolving phlegm, strengthening the lung and fixing the surface, and relieving spasmolysis by wind.
