Lighting the way to sustainable development: Physiological response and light control strategy in microalgae-based wastewater treatment under illumination.

The Sustainable Development Goals link pollutant control with carbon dioxide reduction. Toward the goal of pollutant and carbon reduction, microalgae-based wastewater treatment (MBWT), which can simultaneously remove pollutants and convert carbon dioxide into biomass with value-added metabolites, has attracted considerable attention. The photosynthetic organism microalgae and the photobioreactor are the functional body and the operational carrier of the MBWT system, respectively; thus, light conditions profoundly influence its performance. Therefore, this review takes the general rules of how light influences the performance of MBWT systems as a starting point to elaborate the light-influenced mechanisms in microalgae and the light control strategies for photobioreactors from the inside out. Wavelength, light intensity and photoperiod solely or interactively affect biomass accumulation, pollutant removal, and value-added metabolite production in MBWT. Physiological processes, including photosynthesis, photooxidative damage, light-regulated gene expression, and nutrient uptake, essentially explain the performance influence of MBWT and are instructive for specific microalgal strain improvement strategies. In addition, light causes unique reactions in MBWT systems as it interacts with components such as photooxidative damage enhancers present in types of wastewater. In order to provide guidance for photobioreactor design and light control in a large-scale MBWT system, wavelength transformation, light transmission, light source distribution, and light-dark cycle should be considered in addition to adjusting the light source characteristics. Finally, based on current research vacancies and challenges, future research orientation should focus on the improvement of microalgae and photobioreactor, as well as the integration of both.

Unusual uranium biomineralization induced by green algae: Behavior investigation and mechanism probe.

As a biosorbent, algae are frequently used for the biotreatment or bioremediation of water contaminated by heavy metal or radionuclides. However, it is unclear that whether or not the biomineralization of these metal or radionuclides can be induced by algae in the process of bioremediation and what the mechanism is. In this work, Ankistrodsemus sp. has been used to treat the uranium-contaminated water, and more than 98% of uranium in the solution can be removed by the alga, when the initial uranium concentration ranges from 10 to 80 mg/L. Especially, an unusual phenomenon of algae-induced uranium biomineralization has been found in the process of uranium bioremediation and its mineralization mechanism has been explored by multiple approaches. It is worth noticing that the biomineralization of uranium induced by Ankistrodsemus sp. is significantly affected by contact time and pH. Uranium is captured rapidly on the cell surface via complexation with the carboxylate radical, amino and amide groups of the microalgae cells, which provides nucleation sites for the precipitation of insoluble minerals. Uranium stimulates Ankistrodsemus sp. to metabolize potassium ions (K+), which may endow algae with the ability to biomineralize uranium into the rose-like compreignacite (K2[(UO2)6O4(OH)6]•8H2O). As the time increased, the amorphous gradually converted into compreignacite crystals and a large number of crystals would expand over both inside and outside the cells. To the best of our knowledge, this is the first investigated microalgae with a time-dependent uranium biomineralization ability and superior tolerance to uranium. This work validates that Ankistrodsemus sp. is a promising alga for the treatment of uranium-contaminated wastewater.

Flow cytometry and 5-ethynyl-2'-deoxyuridine (EdU) labeling to detect the cell cycle dynamics of Phaeodactylum tricornutum under light.

Cell cycle studies in plants and algae are highly dependent on reliable methods for detecting cellular DNA replication. With its short growth cycle and ease of genetic transformation, Phaeodactylum tricornutum is an important model organism for the study of pennate diatoms. Here we explored two different methods to detect the cell cycle of P. tricornutum, one using SYBR-green I to via flow cytometry, and the other using EdU labeling to observe cell cycle changes under fluorescence microscopy. Both EdU labeling fluorescence microscopy and SYBR-green I staining flow cytometry accurately indicated that the cells of P. tricornutum enter the G2/M phase after 12 h of light exposure. The results indicate that SYBR Green I was an adequate detection method for nuclear DNA quantitation in cells of P. tricornutum using a flow cytometer and without RNase A treatment. In addition, EdU can be applied to P. tricornutum to reliably detect cell proliferation. Besides, Mg-ProtoIX was able to reverse the cell cycle division inhibition of P. tricornutum and allow the nuclear DNA replication to proceed normally. Taken together, the photoperiodic division time point was clearly identified, which sheds light on the regulation of cell division mechanism in P. tricornutum.

A novel freeze-dried natural microalga powder for highly efficient removal of uranium from wastewater.

It is of great significance to develop convenient methods and low-cost materials to remove uranium from wastewater. Ankistrodesmus sp., an easy growing green alga, was employed for highly efficient removal of uranium from aqueous solution. The biosorption results under different experimental condition indicate that the alga possess outstanding uranium adsorption ability (qmax = 601.2 mg g-1). Moreover, Ankistrodesmus sp. could be effectively regenerated with hydrochloric acid solution (0.1 M) and used again for uranium adsorption. Even in simulated mine water with various coexisting ions, Ankistrodesmus sp. also exhibits high removal efficiency (95.6%) towards uranium. Furthermore, the adsorption behavior of uranium by alga could be described in the Freundlich isotherms model and the adsorption process was consistent with the pseudo-second-order kinetics model. The characteristic of Fourier transform infrared spectrum, scanning electron microscopy, transmission electron microscope and X-ray photoelectron spectroscopy reveal that -NH2, -COOH, -CONH2 and C-O groups have participated in biosorption process. Therefore, complexation, electrostatic adsorption and ions exchange are the dominated action of uranium biosorption in the algae. All findings in this work suggest that Ankistrodesmus sp. can be a promising candidate for the effective and practical application in field of disposed uranium contamination.

The direct pyrolysis and catalytic pyrolysis of Nannochloropsis sp. residue for renewable bio-oils.

Nannochloropsis sp. (a kind of green microalga) residue was pyrolyzed without catalyst or with different amount of HZSM-5 catalyst in a fixed bed reactor in nitrogen flow. The effects of pyrolysis parameters such as temperature and catalyst-to-material ratio on product yields were studied. The bio-oils obtained were analyzed by elemental, GC-MS and FTIR analysis. The results indicated that the bio-oils from catalytic pyrolysis of Nannochloropsis sp. residue (BOCP) had lower oxygen content (19.5 wt.%) and higher heating-value (32.7 MJ kg(-1)) than those obtained from direct pyrolysis (BODP) which had an oxygen content of 30.1 wt.% and heating-value of 24.6 MJ kg(-1). The BODP mainly consisted of long carbon chain compounds with various terminal groups (LCTG), while the BOCP mainly consisted of aromatic hydrocarbons. These properties of bio-oils demonstrated that the Nannochloropsis sp. residue can be used as a renewable energy resource and chemical feedstock.

Fatty acid desaturases from the microalga Thalassiosira pseudonana.

Analysis of a draft nuclear genome sequence of the diatom Thalassiosira pseudonana revealed the presence of 11 open reading frames showing significant similarity to functionally characterized fatty acid front-end desaturases. The corresponding genes occupy discrete chromosomal locations as determined by comparison with the recently published genome sequence. Phylogenetic analysis showed that two of the T. pseudonana desaturase (Tpdes) sequences grouped with proteobacterial desaturases that lack a fused cytochrome b5 domain. Among the nine remaining gene sequences, temporal expression analysis revealed that seven were expressed in T. pseudonana cells. One of these, TpdesN, was previously characterized as encoding a Delta11-desaturase active on palmitic acid. From the six remaining putative desaturase genes, we report here that three, TpdesI, TpdesO and TpdesK, respectively encode Delta6-, Delta5- and Delta4-desaturases involved in production of the health beneficial polyunsaturated fatty acid DHA (docosahexaenoic acid). Furthermore, we show that one of the remaining genes, TpdesB, encodes a Delta8-sphingolipid desaturase with strong preference for dihydroxylated substrates.

Identification of a long-chain polyunsaturated fatty acid acyl-coenzyme A synthetase from the diatom Thalassiosira pseudonana.

The draft genome of the diatom Thalassiosira pseudonana was searched for DNA sequences showing homology with long-chain acyl-coenzyme A synthetases (LACSs), since the corresponding enzyme may play a key role in the accumulation of health-beneficial polyunsaturated fatty acids (PUFAs) in triacylglycerol. Among the candidate genes identified, an open reading frame named TplacsA was found to be full length and constitutively expressed during cell cultivation. The predicted amino acid sequence of the corresponding protein, TpLACSA, exhibited typical features of acyl-coenzyme A (acyl-CoA) synthetases involved in the activation of long-chain fatty acids. Feeding experiments carried out in yeast (Saccharomyces cerevisiae) transformed with the algal gene showed that TpLACSA was able to activate a number of PUFAs, including eicosapentaenoic acid and docosahexaenoic acid (DHA). Determination of acyl-CoA synthetase activities by direct measurement of acyl-CoAs produced in the presence of different PUFA substrates showed that TpLACSA was most active toward DHA. Heterologous expression also revealed that TplacsA transformants were able to incorporate more DHA in triacylglycerols than the control yeast.

Identification of a fatty acid Delta11-desaturase from the microalga Thalassiosira pseudonana.

A set of genomic DNA sequences putatively encoding front-end desaturases were identified by in silico analysis of the draft genome of the marine microalga Thalassiosira pseudonana. Among these candidate genes, an open reading frame named TpdesN was found to be full-length, intronless, and constitutively expressed during cell cultivation. The predicted amino acid sequence of the corresponding protein, TpDESN, exhibited typical features of desaturases involved in the production of polyunsaturated fatty acids (PUFAs) in algae, i.e. a cytochrome b5-like domain at the N-terminus and three conserved histidine-rich motifs in the desaturase domain. Expression of TpDESN in Saccharomyces cerevisiae revealed that this enzyme was not involved in PUFA synthesis, but specifically desaturated palmitic acid 16:0 to 16:1Delta11. To our knowledge, until this report, Delta11-desaturase activity had only been detected in insect cells.