ABSTRACT
This paper is aimed to study the effects of nitrogen form on the growth and quality of Chrysanthemums morifolium at the same nitrogen level. In order to provide references for nutrition regulation of Ch. morifolium in field production, pot experiments were carried out in the greenhouse at experimental station of Nanjing Agricultural University. Five proportions of ammonium and nitrate nitrogen were set up and a randomized block design was applied four times repeatedly. The results showed that the growth and quality of Ch. morifolium were significantly influenced by the nitrogen form. The content of chlorophyll and photosynthesis rate were the highest at the NH4(+) -N /NO3(-) -N ratio of 25:75; The activities of NR in different parts of Ch. -morifolium reached the highest at the NH4(+) - N/NO3(-) -N ratio of 0: 100. The contents of nitrate nitrogen in the root and leaves reached the highest at the NH4(+) -N/NO3(-) -N ratio of 50:50. The activities of GS, GOGAT and the content of amylum increased with the ratio of NO3(-) -N decreasing and reached it's maximum at the NH4 + -N/NO3 - -N ratio of 100: 0. The content of ammonium nitrogen were the highest at the NH4 + -N /NO3 --N ratio of 75: 25, while the content of soluble sugar reached the highest at the NH4(+)-N/NO3(-) -N ratio of 25: 75. The content of flavones, chlorogenic acid and 3,5-O-dicoffeoylqunic acid were 57.2 mg x g(-1), 0.673% and 1.838% respectively, reaching the maximum at the NH4(+) -N /NO3(-) -N ratio of 25:75; The content of luteoloside increased with the ratio of NO3(-) -N increasing and reached it's maximum at the NH4(+) -N/NO3(-) -N ratio of 0: 100. The yield of Ch. morifolium reached it's maximum at the NH4(+) -N /NO3(-) -N ratio of 25:75. Nitrogen form has some remarkable influence on the nitrogen metabolism, photosynthesis and growth, Nitrogen form conducive to the growth and quality of Ch. morifolium at the NH4(+) -N /NO3(-) -N ratio of 25: 75.
Subject(s)
Ammonium Compounds , Metabolism , Pharmacology , Chlorophyll , Metabolism , Chrysanthemum , Metabolism , Flowers , Metabolism , Glutamate Synthase , Metabolism , Glutamate Synthase (NADH) , Metabolism , Glutamate-Ammonia Ligase , Nitrates , Metabolism , Pharmacology , Nitrogen , Metabolism , Pharmacology , Photosynthesis , Plant Leaves , Metabolism , Plant Proteins , Metabolism , Plant Roots , Metabolism , Plant Stems , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To find out the bioactive principles of Alpinia tonkinensis, by investigating its chemical constituents.</p><p><b>METHOD</b>It was extracted with MeOH, distributed by different solvents, and isolated via column chromatography on silica gel.</p><p><b>RESULT</b>Six compounds were elucidated through spectral analysis, which were as follows: 4',7-dimethylkaempferol(I), 5-hydroxy-3',4',7-trimethoxyflavanone(II), kumatakenin(III), 4',5,7-trimethoxyflavonol(IV), ombuine(V), and kaempferol(VI).</p><p><b>CONCLUSION</b>Six flavonoids were isolated from this plant for the first time, and so were four compounds from genus Alpinia.</p>
