ABSTRACT
Objective To observe the clinical efficacy of moxa stick moxibustion in treating diabetic gastroparesis (DGP) due to deficient cold of spleen-stomach, and to discuss the action mechanism.Method Seventy DGP patients were randomized into a treatment group and a control group, 35 cases each. The treatment group was intervened by moxa stick moxibustion, while the control group was given oral administration of Domperidone tablets, 7 d as a treatment course, totally for 2 courses. The DGP clinical symptoms evaluation scale, gastric emptying time and biochemical indexes (contents of glycosylated hemoglobin and serum motilin) were compared before and after the treatment, and the clinical efficacies were compared between the two groups.Result After the treatment, the DGP clinical symptoms evaluation scales, gastric emptying time, and biochemical indexes were changed significantly in both groups (P<0.05). The DGP clinical symptoms evaluation scales, gastric emptying time, and biochemical indexes in the treatment group were significantly different from those in the control group after the treatment (P<0.05).Conclusion Moxa stick moxibustion can improve the quality of life of DGP patients due to deficient cold of spleen-stomach, which is possibly related to the improvement of the glycosylated hemoglobin, serum motilin and gastric emptying time.
ABSTRACT
Objective To explore the effect of different concentrations of ALLN on proliferation and apoptosis of C2C12 myoblasts.Methods After intervention with Ca2+ and ALLN,methyl thiazolyl tetrazolium and flow cytometry were used to determine the effect of Ca2+ and ALLN on the proliferation and apoptosis of C2C12 cells,respectively.The morphological changes of C2C12 myoblasts were observed using Giemsa staining.Results The absorbance of Ca2 + group was significantly lower than that of the control group (P ＜0.05).After 6,12,24,36 hours of intervention,the absorbance in ALLN groups 1 to 7 (cultured in serum-free media containing 16 mmol/L Ca2+ and ALLN at final concentrations of 3.125,6.25,12.5,25,50,100,200 μmol/L) were all significantly higher than that in the 16 mmol/L Ca2+ group (after 6 hours:0.449±0.024,0.472±0.022,0.513 ±0.008,0.540±0.014,0.588±0.016,0.607±0.030,0.700±0.020 vs.0.355 ±0.012,all P =0.000; after 12 hours:0.407 ±0.007,0.414 ±0.006,0.434 ±0.004,0.441 ±0.003,0.460 ±0.010,0.484 ± 0.006,0.525 ± 0.006 vs.0.368 ± 0.027,all P =0.000; after 24 hours:0.436±0.005,0.431 ±0.015,0.441 ±0.006,0.459 ±0.013,0.527 ±0.009,0.581 ±0.005,0.599 ±0.011 vs.0.386 ± 0.007,all P =0.000 ; after 36 hours:0.464 ± 0.022,0.460 ± 0.018,0.461 ± 0.007,0.434 ± 0.020,0.454 ± 0.028,0.479 ± 0.006,0.524 ± 0.011 vs.0.379 ± 0.011,all P =0.000),while no significant differences were observed after 48-72 hours of intervention.After treatment for 36 hours,the apoptosis rate in ALLN 10,50,100,and 200 μmol/L groups were (6.00 ± 1.20) ％,(5.02 ± 1.13) ％,(4.89±1.11)％,and (2.71 ± 1.15)％,all significantly lower than that in the Ca2+ group [(13.70 ±2.30)％] (all P =0.000).Giemsa staining showed apoptotic morphological changes in the Ca2+ group,which were obviously alleviated in the ALLN group.Conclusions Ca2+ at a concentration of 16mmol/L can induce apoptosis of C2C12 cells.In contrast,ALLN can inhibit cell apoptosis and promote proliferation in a time-and dose-dependent manner.
