ABSTRACT
Background@#The emergence of the severe acute respiratory syndrome coronavirus 2 omicron variant has been triggering the new wave of coronavirus disease 2019 (COVID-19) globally. However, the risk factors and outcomes for radiological abnormalities in the early convalescent stage (1 month after diagnosis) of omicron infected patients are still unknown. @*Methods@#Patients were retrospectively enrolled if they were admitted to the hospital due to COVID-19. The chest computed tomography (CT) images and clinical data obtained at baseline (at the time of the first CT image that showed abnormalities after diagnosis) and 1 month after diagnosis were longitudinally analyzed. Uni-/multi-variable logistic regression tests were performed to explore independent risk factors for radiological abnormalities at baseline and residual pulmonary abnormalities after 1 month. @*Results@#We assessed 316 COVID-19 patients, including 47% with radiological abnormalities at baseline and 23% with residual pulmonary abnormalities at 1-month follow-up. In a multivariate regression analysis, age ≥ 50 years, body mass index ≥ 23.87, days after vaccination ≥ 81 days, lymphocyte count ≤ 1.21 × 10 -9 /L, interleukin-6 (IL-6) ≥ 10.05 pg/mL and IgG ≤ 14.140 S/CO were independent risk factors for CT abnormalities at baseline. The age ≥ 47 years, presence of interlobular septal thickening and IL-6 ≥ 5.85 pg/mL were the independent risk factors for residual pulmonary abnormalities at 1-month follow-up. For residual abnormalities group, the patients with less consolidations and more parenchymal bands at baseline could progress on CT score after 1 month. There were no significant changes in the number of involved lung lobes and total CT score during the early convalescent stage. @*Conclusion@#The higher IL-6 level was a common independent risk factor for CT abnormalities at baseline and residual pulmonary abnormalities at 1-month follow-up. There were no obvious radiographic changes during the early convalescent stage in patients with residual pulmonary abnormalities.
ABSTRACT
SARS-CoV-2 infection induces severe disease in a subpopulation of patients, but the underlying mechanisms remain unclear. We demonstrate robust IgM autoantibodies that recognize angiotensin converting enzyme-2 (ACE2) in 18/66 (27%) patients with severe COVID-19, which are rare (2/52; 3.8%) in hospitalized patients who are not ventilated. The antibodies do not undergo class-switching to IgG, suggesting a T-independent antibody response. Purified IgM from anti-ACE2 patients activates complement. Pathological analysis of lung obtained at autopsy shows endothelial cell staining for IgM in blood vessels in some patients. We propose that vascular endothelial ACE2 expression focuses the pathogenic effects of these autoantibodies on blood vessels, and contributes to the angiocentric pathology observed in some severe COVID-19 patients. These findings may have predictive and therapeutic implications. One-sentence summaryACE2 autoantibodies in severe COVID-19 have features of a T-independent immune response, and may mediate vascular damage.
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Researches found that compared with continuous aerobic exercise training (CAET) ,high intensity interval training (HIIT) of short interval possessed more benefits for patients with coronary heart disease (CHD) and low aerobic adaptability ,especially in initial and symptom‐improving stage .Medium and／or long interval HIIT may be beneficial for CHD patients with high aerobic fitness ,which can be used in symptom‐improving and maintenance stage .The present article put forward HIIT (stage II to III ) personalized model for CHD patients and explored more optimal cardiac rehabilitation program according to patients＇clinical state .
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Objective: To explore CT characteristics of mycobacterium intracellulare pulmonary diseases compared with mycobacterium kansasii, mycobacterium abscess/chelonei pulmonary diseases. Methods: CT images of 35 patients with mycobacterium intracellulare pulmonary diseases, 18 with mycobacterium kansasii pulmonary disease and 22 with mycobacterium abscess/chelonei pulmonary disease confirmed by clinical data and laboratory tests were retrospectively analyzed, and imaging findings were evaluated and compared. Results: Mycobacterium intracellulare pulmonary disease involved both lungs (33/35, 94.29%), multiple lobes (18/35, 51.43%) or all lobes (16/35, 45.71%). CT characteristics included cord shadow (34/35, 97.14%), air space consolidation (33/35, 94.29%), centrilobular nodules or tree in bud (32/35, 91.43%), thickened pleura (32/35, 91.43%), ground glass opacity (31/35, 88.57%), traction bronchiectasis (30/35, 85.71%), non-traction bronchiectasis (25/35, 71.43%), nodes (24/35, 68.57%), calcification (24/35, 68.57%) and cavity (23/35, 65.71%). Ground glass opacity was more frequently observed in mycobacterium intracellulare than mycobacterium kansasii (P=0.001) and mycobacterium abscess/chelonei pulmonary disease (P<0.001). Walls of the cavity were thicker in mycobacterium intracellulare than mycobacterium kansasii (P=0.019) and mycobacterium abscess/chelonei pulmonary disease (P=0.024). Calcifications were more frequently observed in mycobacterium intracellulare than in mycobacterium kansasii (P=0.014) and mycobacterium abscess/chelonei pulmonary disease (P=0.007). Conclusion: Compared with mycobacterium kansasii and abscess/chelonei pulmonary disease, CT findings of mycobacterium intracellulare have certain characteristics, which may be helpful to differential diagnosis.
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Objective To study the effect of bel-2 siRNA on apoptosis of HL-60 cells.Methods bcl-2 siRNA was synthesized in vitro transcription with silencer siRNA construction kit.The synthesized siRNA was transfected into HL-60 cells with Amine siPORT transfection.We used MTT flow cytometer and hoechst 33258 flourescence stainning t0 evaluate cell proliferation and apoptosis. Results.Bcl-2 siRNA could partially inhibit the growth of HL-60 cells.After incubated with bcl-2 siRNAl for 48 hours,HL-60 cells exhibited morphologic characteristic of apoptosis including chromatin condensation,crescents formation and nuclear fragmentation.Conclusion Effective bcl-2 siRNA can induce apoptosis and inhibit cell proliferation.
