ABSTRACT
miRNA was 20～25 nt endogenous non-coding RNA. miRNAs are encoded small RNAs that hybridize with messenger RNAs, resulting in degradation or translational inhibition of targeted transcripts. In order to investigate the function of miR-138 on mouse mammary epithelial cells, technique for gene silencing-miRNA inhibitor (anti-miRNA) was applied to make miR-138 silence, qRT-PCR was showed valid for inhibitor miR-138. And Western blot, CASY(r)-technology was put in use to study some change of mouse mammary epithelial cells after miR138 inhibitor. It was shown that miR-138 suppresses the exepress of PRL-R(P
ABSTRACT
Objective To systematically study the development and morphological changes of Taenia solium cysticerci in the period from the appearance to maturation. Methods Pigs were orally infected with T.solium eggs. Autopsies were made periodically to get tissue specimens and examine the distribution of cysticerci. Cysticerci were excised, pressed between two microscopic slides for microscopical observation. Sections were also made for histological study. Results Cysticerci distributed mainly in muscles, secondly in brain, liver, lung and kidney. The size of cysticerci was highly variable at different parts or even in the same part of the same host. The early stage cysticercus was observed on the 19th day without suckers and hooklets on the scolex region, and was only found in skeletal muscles. Histological examination revealed that early development of the scolex became obvious. No cysticerci were found in other tissues. At 30 d, the cysticerci possessed hooklets and brood suckers. With a longer duration of infection, the diameter of suckers and length of hooklets both increased. Larger suckers and more folds on scolex appeared 60 d later. Morphologically, cysticerci after 60 d were similar to those found at 60 d. Degenerated cysticerci were found at every stage. Conclusion Cysticerci appear in about 19 days after the pig was infected with eggs of T.solium and the duration needed for maturation is about 60 d.
ABSTRACT
Objective To analyze the sibling relationship and genetic diversity of four certified Radix Gentianae (RG) species by RAPD and ISSR methods. It provided molecular biological proof for species identification and breeding of certified RG. Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data. Results Ten RAPD primers and four ISSR primers were selected respectively from 100 RAPD primers and 32 ISSR primers. The statistic data were analyzed to construct cluster analysis dendrogram by genetic distance UPGMA method. Conclusion The PCR reaction systems of this experiment gets ideal RAPD and ISSR results that suit to the analysis of certified RG species in genetic diversity and sibling relationship.
