ABSTRACT
Polyamines play an important role in stress response. In the pathway of polyamines synthesis, S-adenosylmethionine decarboxylase (SAMDC) is one of the key enzymes. In this study, a full length cDNA of SAMDC (AhSAMDC) was isolated from peanut (Arachis hypogaea L.). Phylogenetic analysis revealed high sequence similarity between AhSAMDC and SAMDC from other plants. In peanut seedlings exposed to sodium chloride (NaCl), the transcript level of AhSAMDC in roots was the highest at 24 h that decreased sharply at 72 and 96 h after 150 mM NaCl treatment. However, the expression of AhSAMDC in peanut leaves was significantly inhibited, and the transcript levels in leaves were not different compared with control These results implied the tissue-specific and time-specific expression of AhSAMDC. The physiological effects and functional mechanism of AhSAMDC were further evaluated by overexpressing AhSAMDC in tobaccos. The transgenic tobacco lines exhibited higher germination rate and longer root length under salt stress. Reduced membrane damage, higher antioxidant enzyme activity, and higher proline content were also observed in the transgenic tobacco seedlings. What's more, AhSAMDC also led to higher contents of spermidine and spermine, which can help to scavenge reactive oxygen species. Together, this study suggests that AhSAMDC enhances plant resistance to salt stress by improving polyamine content and alleviating membrane damage.
Subject(s)
Adenosylmethionine Decarboxylase , Arachis , Nicotiana , Plants, Genetically Modified , Salt Stress , Adenosylmethionine Decarboxylase/genetics , Adenosylmethionine Decarboxylase/metabolism , Arachis/enzymology , Arachis/genetics , Gene Expression Regulation, Plant , Phylogeny , Plants, Genetically Modified/drug effects , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , Salt Stress/genetics , Sodium Chloride/toxicity , Nicotiana/drug effects , Nicotiana/enzymology , Nicotiana/geneticsABSTRACT
Forty-three single fibres were recorded from cat optic tract. They were tested with flashing lines of different lengths and/or spots of different dimensions. The area of centre excitation and of surround inhibition were represented in the length (or dimension)-response functions by the steep rising and falling phases, respectively. For most of the cells, a secondary gentle rise was seen when the stimulus extended beyond the inhibitory surround, indicating an extensive disinhibitory region (DIR) outside the classical receptive field (RF). Disinhibition appeared and developed concurrently with surround inhibition at stimulus intensities between 3 and 30 cd/m2, being represented in the phasic or the tonic component of responses depending on the cell type recorded.
