Cadmium contamination decreased bacterial network complexity and stability in coastal reclamation areas.

Cadmium(Cd) contamination can exert significantly adverse effects on soil microbiota in reclaimed areas, however, its effects on bacterial network structure are still limitedly understood. Here we collected soil samples from typical reclaimed wetlands (RW) and ditch wetlands (DW) in coastal reclamation areas and examined the effects of Cd contamination on the bacterial network complexity and stability. The results showed that the bacterial networks were destabilized by the Cd contamination, while bacteria in DW soils showed robust invulnerability characterized by higher node constancy and compositional stability compared with RW soils. Soil bacteria resisted Cd stress by forming a network with intensive connections in the module but sparser connections among the modules. Especially, network modularity was higher in DW soils than in RW soils, but made it more vulnerable to nodes removal. In addition, Cd contamination promoted bacterial positive cohesion but decreased negative cohesion in RW soils. Flavobacteriaceae, Xanthomonadaceae, and Alcaligenaceae were identified as core phylotypes, which played pivotal roles in regulating interspecies interactions due to higher contributions to cohesion and significant correlations with soil nutrients. The findings of this work indicate the changes of bacterial network structure and the indispensable role of core phylotypes in regulating interactions and maintaining network sustainability under Cd contamination.

Generation of a 25-line flattened optical frequency comb and its coherent beating properties.

A 25-line flattened optical frequency comb (OFC) is generated by cascading Mach-Zehnder modulators, and its coherent beating properties are investigated. The effect of bias voltages and the power of radio frequency signals on the flatness of the OFC, and the phase relationships between each comb line are theoretically deduced. In the experiment, a 25-line OFC with a flatness of less than 1 dB and an optical sideband suppression ratio of 12 dB is successfully demonstrated, and the agreement between simulation and experimental results supports the validity of the model for analysis of the flatness. The measured amplitude of harmonic signals by coherent beating of the generated 25-line OFC matches well with the theoretical prediction. A phase noise of -105.31dBc/Hz at 10 kHz frequency offset for the 12th harmonic signal almost without phase noise degradation proves the high coherence of the generated OFC lines.