Elemental stoichiometry (C, N, P) of soil in the Yellow River Delta nature reserve: Understanding N and P status of soil in the coastal estuary.

The Yellow River Delta Nature Reserve (YNR), which includes two separated regions: part of the old Yellow River Delta (OYD) and part of the current Yellow River Delta (CYD), was established to protect coastal wetlands in the coastal estuary. A total of 120 plots were sampled in the YNR in April 2016, and the spatial patterns of soil C, N and P contents and their stoichiometric ratios (C:N (RCN), C:P (RCP) and N:P (RNP)) were studied and interpolated using the Ordinary Kriging method. Results indicated that the soil elemental contents and stoichiometric ratios showed high spatial heterogeneity and large variations. The mean C:N:P ratio (RCNP) was ~ 64.7:2.3:1 in OYD, and ~ 64.5:2.0:1 in CYD, respectively, and a well-constrained RCP ratio ~ 65:1 was found in the 0-50 cm soil depth within the YNR. N showed greater variation than C and P. Furthermore, N contents in the 0-5 cm soil layer of OYD were significantly higher than that of CYD (F = 4.79, p = 0.03); RCN in 0-5 cm, 5-10 cm layers of OYD was significantly lower than those in the same layers of CYD (F = 4.75, p = 0.03; F = 5.18, p = 0.02, respectively). RNP in 0-5 cm soil layer of OYD was notably higher than that of CYD (F = 4.88, p = 0.03). These results were due to the combined actions of sedimentation, reclamation and fertilization. Finally, we concluded that a longer reclamation and fertilization history led to decreased RCN in coastal estuary soils, confirmed that the soil of the YNR exhibits N limitation, and suggested that the soil RCN and RNP could be good indicators of the anthropogenic improvement status during soil development in this coastal estuary.

Environmental threats induced heavy ecological burdens on the coastal zone of the Bohai Sea, China.

The environment of the Bohai Sea is under enormous pressure because of rapid economic and urban development associated with increased population inhabiting the coastal zone. Environmental threats to the coastal ecosystem were analyzed using 2006-2017 statistical/monitoring data from the State Oceanic Administration, China. The results showed that harmful algal blooms occurred a total of 104 times during the period of 2006-2017, for a cumulative area of more than 21,275 km2. The main environmental threats came from offshore oil and gas production in the form of hydrocarbon pollution during extraction, as well as from urban wastewater and sewage. Oil pollution, mainly generated from spills, offshore oil platforms and large number of vessels/ports, was found to cause very severe negative impacts on the environment. Another threat is from excessive groundwater exploitation which has resulted in seawater intrusion and soil salinization occurrence in more than 90% of coastal areas around the Bohai Sea. The maximum distance of intrusion by seawater and soil salinization was more than 40 and 32 km inland, respectively. Contamination by terrestrial pollutants was identified as another threat affecting the environment quality of the Bohai Sea. Approximately 840,000 t of pollutants were carried into the sea by major rivers annually for 2010-2017. The standard discharge rate of terrestrial-source sewage outlets did not exceed 50%; however, only 13.12% of sea areas adjacent to sewage outlets (rivers) met the environmental quality requirements for functional marine areas. The results also showed the frequency of storm surges in the Bohai Sea which was 8.83 times per year and the resulting annual direct economic losses reached (RMB) 1.77 billion for 2006-2017. The results highlight the urgent need to implement an ecological management strategy to reduce the heavy ecological burdens in the coastal zone of the Bohai Sea.

Impacts of inland pollution input on coastal water quality of the Bohai Sea.

Inland pollutants input is a key impact factor for the coastal water quality of the Bohai Sea. The coastal and inland water pollutant inputs were analyzed by using monitoring data of recent years from the State Oceanic Administration. The results showed that more than 56% of the Bohai Sea area was unclean seawater in 2012, although the water quality improved gradually after that time. In 2017, about one-third of the Bohai Sea area still had unclean seawater. Inorganic nitrogen, reactive phosphate, and petroleum hydrocarbons are the main pollutants in the seawater. A total of approximately 840,000 t of pollutants was transported to the sea each year by major rivers during 2010-2017. Significant correlations (p < 0.05) were found between the third-grade level seawater area and the pollutants of CODcr, petroleum, NO3--N, NH4+-N, NO2--N, Cu, and Pb and between the inferior fourth-grade level seawater area and the pollutants of petroleum, NO2--N, Pb, and NO3--N. The standard discharge rate of terrestrial-source sewage outlets was no more than 50%. The low standard discharge rate of the major terrestrial-source sewage pollutants of CODcr, NH4+-N, TP, BOD5, and SS caused more than 80% of the monitored sea areas adjacent to the selected key sewage outlets to not meet the water quality requirements of the marine functional area. The results suggest that implementing a coastal water management plan is necessary to reduce the heavy ecological burdens on the coastal zone of the Bohai Sea.

Transport characteristics of salt ions in soil columns planted with Tamarix chinensis under different groundwater levels.

The groundwater level is the main factor affecting the distribution of soil salinity and vegetation in the Yellow River Delta (YRD), China, but the response relationship between the spatial distribution of soil salt ions and the groundwater level in the soil-Tamarix chinensis system remains unclear. In order to investigate the patterns of soil salt ions responding to groundwater levels, in the 'groundwater-soil-T. chinensis' system. Soil columns planted with T. chinensis, a constructive species in the YRD, were taken as the study object, and six groundwater levels (0.3, 0.6, 0.9, 1.2, 1.5 and 1.8 m) were simulated under saline mineralization. The results demonstrated the following: As affected by groundwater, Na+ and Cl- were the main ions in the T. chinensis-planted soil column, with a trend of decreasing first and then increasing by the increase of soil depth. However, the contents of K+ and NO3- gradually decreased and CO32-+HCO3- gradually increased. As affected by groundwater evaporation, all the salt ions except CO32-+HCO3- exhibited different degrees of surface aggregation in the 0-20 cm layer. However, due to the impact of root uptake, the contents of the salt ions rapidly decreased in the root distribution layer (20-50 cm soil layer), which rendered a turning-point layer that was significantly lower than the surface soil layer; such decreases in ion contents showed a relatively large rate of variation. In the whole T. chinensis-planted soil column, with increasing groundwater level, the contents of Na+, Cl-, Ca2+, Mg2+, and NO3- all tended to first decrease, then increase and decrease again, but the content of CO32-+HCO3- first decreased and then increased. Therefore, the 0.9 m groundwater level was the turning point at which the main salt ions underwent significant changes. The contents of Na+, Cl-, Ca2+ and Mg2+ in the T. chinensis planted soil column exhibited moderate variability (14.46%111.36%) at most groundwater level except less than 0.9 m. Therefore, planting T. chinensis could effectively reduce the accumulation of salt ions in the 20-50 cm soil layer with a concentrated root distribution, suggesting that the planting depth of T. chinensis should be greater than 20 cm under saline mineralization. This study can provide references for the control of soil secondary salinization and the management of T. chinensis seedling cultivation under saline mineralization.

Isolation, identification of a laccase-producing fungal strain and enzymatic properties of the laccase.

A new type of thermostable laccase was isolated from Paraphoma sp. GZS18, and its partial enzymatic properties were determined. A strain GZS18 of laccase with high yield was screened from forest soil and identified as Paraphoma sp. GZS18 through morphological characteristics and ITS sequence analysis. The laccase of Paraphoma sp. GZS18 (Lac-P) was obtained through cation-anion exchange chromatography, gel filtration chromatography, and other purification processes. The testing result shows that Lac-P is a single protein of 75 kDa, and the 11 amino acid sequences in the N-terminal are AXaVSVASREMT (Xa was the non-standard protein). The optimum temperature and optimum pH of lac-P activity are substrate-independent. The temperature is in the range of 50-70 °C, and pH has high catalytic efficiency in the acidic range. Lac-P has good stability in the temperature and pH. The half time at 70-60 °C is 1.5 and 4 h, respectively. At pH 6-9 and room temperature, there is more than 80% activity 24 h later. Lac-P is tolerant of most metal ions and low concentrations of inhibitors but is inhibited by Hg2+, Fe2+ and NaN3. The laccase from Paraphoma sp. GZS18 at high temperature and pH 6-9, with strong stability, has better industrial application characteristics.

Influences of micro-geomorphology on the stoichiometry of C, N and P in Chenier Island soils and plants in the Yellow River Delta, China.

Studies have indicated that consistent or well-constrained (relatively low variability) carbon:nitrogen:phosphorus (C:N:P) ratios exist in large-scale ecosystems, including both marine and terrestrial ecosystems. Little is known about the C, N and P stoichiometric ratios that exist in the soils and plants of Chenier Island in the Yellow River Delta (YRD). We examined the distribution patterns and relationships of C, N and P stoichiometry in the soils and plants of Chenier Island, as well as the potential influences of the island's micro-geomorphology. Based on a study of four soil profile categories and Phragmites australis and Suaeda heteroptera plant tissues, our results showed that micro-geomorphology could leave a distinct imprint on the soil and plant elemental stoichiometry of Chenier Island; significant variation in the atomic C:N:P ratios (RCNP) existed in soils and plants, indicating that the RCNP values in both the soil and plants are not well constrained at the Chenier Island scale. RCN and RCP in Chenier Island soils were high, whereas the RNP values were comparatively low, indicating that the ecosystems of Chenier Island are nutrient-limited by N and P. However, the RNP values in P. australis and S. heteroptera plant tissues were high, suggesting that the plants of Chenier Island are nutrient-limited by P. Finally, we suggest that soil and plant N:P ratios may be good indicators of the soil and plant nutrient status during soil development and plant growth, which could be a useful reference for restoring the degraded soils of Chenier Island.

Ecological effects of roads on the plant diversity of coastal wetland in the Yellow River Delta.

The 26 sample sites in 7 study plots adjacent to asphalt road and earth road in coastal wetland in the Yellow River Delta were selected to quantify plant diversity using quadrat sampling method in plant bloom phase of July and August 2012. The indice of ß T and Jaccard's coefficient were applied to evaluate the species diversity. The results showed that the plant diversities and alien plants were high in the range of 0-20 m to the road verge. There were more exotics and halophytes in plots of asphalt roadside than that of earth roadside. However, proportion of halophytes in habitats of asphalt roadsides was lower than that of earth roadside. By comparing ß-diversity, there were more common species in the asphalt roadsides than that in the earth roadsides. The similarity of plant communities in studied plots of asphalt roadsides and earth roadsides increased with increasing the distance to road verge. The effect range of roads for plant diversity in study region was about 20 m to road verge. Our results indicate that the construction and maintenance of roads in wetland could increase the plant species diversities of communities and risk of alien species invasion.

N2O emissions from an apple orchard in the coastal area of Bohai Bay, China.

Using static chambers and gas chromatography, nitrous oxide (N2O) fluxes from an apple orchard soil in the Bohai Bay region of China were measured from February 2010 to February 2011. In this study, two nitrogen (N) fertilizer treatments were designed--without (CK) or with (SN) synthetic N fertilizers (800 kg N ha(-1)). The annual cumulative N2O emissions from CK and SN were 34.6 ± 3.0 (mean ± standard error) and 44.3 ± 6.0 kg N2O-N ha(-1), respectively. Such high emissions resulted from the intensive N fertilization in the experimental and previous years. The direct emission factor (EFd) of N2O induced by the applied synthetic N fertilizers was 1.2%. The EFd is within the range of previous studies carried out in other croplands, which suggests that it is reasonable to estimate regional N2O emissions from apple orchards using the EFd obtained in other croplands. In addition, significant positive correlations existed between N2O fluxes and soil temperatures or soil dissolved organic carbon contents.

A meta-analysis of the bacterial and archaeal diversity observed in wetland soils.

This study examined the bacterial and archaeal diversity from a worldwide range of wetlands soils and sediments using a meta-analysis approach. All available 16S rRNA gene sequences recovered from wetlands in public databases were retrieved. In November 2012, a total of 12677 bacterial and 1747 archaeal sequences were collected in GenBank. All the bacterial sequences were assigned into 6383 operational taxonomic units (OTUs 0.03), representing 31 known bacterial phyla, predominant with Proteobacteria (2791 OTUs), Bacteroidetes (868 OTUs), Acidobacteria (731 OTUs), Firmicutes (540 OTUs), and Actinobacteria (418 OTUs). The genus Flavobacterium (11.6% of bacterial sequences) was the dominate bacteria in wetlands, followed by Gp1, Nitrosospira, and Nitrosomonas. Archaeal sequences were assigned to 521 OTUs from phyla Euryarchaeota and Crenarchaeota. The dominating archaeal genera were Fervidicoccus and Methanosaeta. Rarefaction analysis indicated that approximately 40% of bacterial and 83% of archaeal diversity in wetland soils and sediments have been presented. Our results should be significant for well-understanding the microbial diversity involved in worldwide wetlands.

Soil phosphorus forms and profile distributions in the tidal river network region in the Yellow River Delta estuary.

Modified Hedley fraction method was used to study the forms and profile distribution in the tidal river network region subjected to rapid deposition and hydrologic disturbance in the Yellow River Delta (YRD) estuary, eastern China. The results showed that the total P (Pt) ranged from 612.1 to 657.8 mg kg(-1). Dilute HCl extractable inorganic P (Pi) was the predominant form in all profiles, both as absolute values and as a percentage of total extracted Pi. The NaOH extractable organic P (Po) was the predominant form of total extracted Po, while Bicarb-Pi and C.HCl-Po were the lowest fractions of total extracted Pi and Po in all the P forms. The Resin-P concentrations were high in the top soil layer and decreased with depth. The Pearson correlation matrix indicated that Resin-P, Bicarb-Pi, NaOH-Pi, and C.HCl-Pi were strongly positively correlated with salinity, TOC, Ca, Al, and Fe but negatively correlated with pH. The significant correlation of any studied form of organic P (Bicarb-Po, NaOH-Po, and C.HCl-Po) with geochemical properties were not observed in the study. Duncan multiple-range test indicated that the P forms and distribution heterogeneity in the profiles could be attributed to the influences of vegetation cover and hydrologic disturbance.