ABSTRACT
As one of the important members of reactive oxygen species, ONOO- plays a crucial role in signal transduction, immune response, and other physiological activities. Aberrant changes in ONOO- levels in the living organism are usually associated with many diseases. Therefore, it is important to establish a highly selective and sensitive method for the determination of ONOO- in vivo. Herein, we designed a novel ratio near-infrared fluorescent probe for ONOO- by directly conjugating dicyanoisophorone (DCI) to hydroxyphenyl-quinazolinone (HPQ). Surprisingly, HPQD was unaffected by environmental viscosity and responded rapidly to ONOO- within 40 s. The linear range of ONOO- detection was from 0 µM to 35 µM. Impressively, HPQD did not react with reactive oxygen species and was sensitive to exogenous/endogenous ONOO- in live cells. We also investigated the relationship between ONOO- and ferroptosis and achieved in vivo diagnosis and efficacy evaluation of mice model of LPS-induced inflammation, which showed promising prospects of HPQD in ONOO--related studies.
