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1.
J Gen Virol ; 95(Pt 6): 1396-1407, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24676420

ABSTRACT

The TATA-box-binding protein (TBP) plays a key role in initiating eukaryotic transcription and is used by many viruses for viral transcription. We previously reported increased TBP levels during infection with the baculovirus Autographa californica multicapsid nuclear polyhedrovirus (AcMNPV). The TBP antiserum used in that study, however, cross-reacted with a baculoviral protein. Here, we reported that increased amounts of nuclear TBP were detected upon infection of Spodoptera frugiperda and TN-368 cells with a TBP-specific antiserum. TBP levels increased until 72 h post-infection (p.i.), whilst tbp transcripts decreased by 16 h p.i., which suggested a virus-induced influence on the TBP protein levels. To address a potential modification of the TBP degradation pathway during infection, we investigated the possible role of viral ubiquitin. Infection studies with AcMNPV recombinants carrying a mutated viral ubiquitin gene revealed that the TBP increase during infection was not altered. In addition, pulse-chase experiments indicated a high TBP half-life of ~60 h in uninfected cells, suggesting that a virus-induced increase of TBP stability was unlikely. This increase in TBP correlated with a redistribution to nuclear domains resembling sites of viral DNA synthesis. Furthermore, we observed colocalization of TBP with host RNA polymerase (RNAP) II, but only until 8 h p.i., whilst TBP, but not RNAPII, was present in the enlarged replication domains late during infection. Thus, we suggested that AcMNPV adapted a mechanism to accumulate the highly stable cellular TBP at sites of viral DNA replication and transcription.


Subject(s)
Insect Proteins/metabolism , Nucleopolyhedroviruses/physiology , Nucleopolyhedroviruses/pathogenicity , TATA-Box Binding Protein/metabolism , Active Transport, Cell Nucleus , Animals , Cell Line , DNA Replication , Host-Pathogen Interactions , Nucleopolyhedroviruses/genetics , Protein Stability , Proteolysis , Sf9 Cells , Spodoptera , Ubiquitin/metabolism , Virus Replication
2.
J Virol ; 81(11): 6122-7, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17376916

ABSTRACT

Baculovirus DNA binding protein (DBP) binds preferentially single-stranded DNA in vitro and colocalizes with viral DNA replication sites. Here, its putative role as viral replication factor has been addressed by RNA interference. Silencing of DBP in Autographa californica multiple nucleopolyhedrovirus-infected cells increased expression of LEF-3, LEF-4, and P35. In contrast, expression of the structural genes coding for P39 and polyhedrin was suppressed while expression of genes coding for P10 and GP64 was unaffected. In the absence of DBP, viral DNA replication sites were formed, indicating replication of viral DNA. Electron microscopy studies, however, revealed a loss of formation of polyhedra and virus envelopment, suggesting that the primary role of DBP is viral formation rather than viral DNA replication.


Subject(s)
Baculoviridae/metabolism , DNA, Viral/biosynthesis , DNA-Binding Proteins/antagonists & inhibitors , DNA-Binding Proteins/genetics , Gene Silencing , Animals , Cell Line , DNA Replication/physiology , DNA, Viral/antagonists & inhibitors , DNA-Binding Proteins/physiology , Virus Replication/physiology
3.
J Virol ; 80(8): 4168-73, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16571832

ABSTRACT

Baculovirus lef-4 encodes one subunit of the viral RNA polymerase. Here, we demonstrate the essential nature of LEF-4 by RNA interference and bacmid knockout technology. Silencing of LEF-4 in wild-type virus-infected cells suppressed expression of structural genes, while early expression was unaffected, demonstrating its essential role in late gene expression. After transfection of insect cells with lef-4 mutant bacmid, no viral progeny was produced, further defining its central role in infection. Cotransfection with wild-type lef-4 plasmid restored normal replication, but plasmid encoding a guanyltransferase-deficient version failed to rescue. These results emphasize the importance of the mRNA capping function of LEF-4.


Subject(s)
DNA-Directed RNA Polymerases/physiology , Nucleopolyhedroviruses/genetics , Nucleotidyltransferases/physiology , Viral Proteins/physiology , Animals , Gene Expression , RNA Caps/physiology , Spodoptera , Transcription, Genetic , Viral Proteins/genetics
4.
Virus Res ; 115(2): 207-13, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16271277

ABSTRACT

Host RNA polymerase II (RNAP II) is responsible for viral transcription of the herpes simplex virus type 1 (HSV-1) genome and is relocalized to viral DNA replication compartments. Thus, we investigated whether TATA-binding protein (TBP) and TBP-associated factors (TAFs) are recruited to sites of viral transcription and replication and whether TBP/TAF expressions are influenced upon infection. The protein levels of TBP, hsTAF1/TAF(II)250, hsTAF4/TAF(II)135, and hsTAF5/TAF(II)100 were constant during the early phase of infection and started to decrease late during infection. Only for hsTAF7/TAF(II)55 we sometimes observed a decrease already at 4-8h postinfection (p.i.). Concomitantly with the relocalization of RNAP II, TBP and hsTAFs were redistributed to sites of viral DNA replication and transcription. In the absence of viral DNA replication TBP/hsTAFs were present in distinct nuclear dots, however, enlargement of the nuclear structures did not take place. Our results show that HSV-1 infection has no influence on the protein levels of TFIID components and leads to a redistribution of TBP and hsTAFs to prereplicative sites that enlarge to viral DNA replication compartments.


Subject(s)
Cell Nucleus/chemistry , DNA Replication , Herpesvirus 1, Human/physiology , TATA-Binding Protein Associated Factors/analysis , TATA-Box Binding Protein/analysis , Virus Replication , Cells, Cultured , DNA, Viral/metabolism , Fibroblasts , Humans , Immunohistochemistry , Microscopy, Confocal , RNA Polymerase II/analysis , Transcription, Genetic
5.
J Virol ; 76(21): 11123-7, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12368354

ABSTRACT

During the infection cycle of Autographa californica multicapsid nuclear polyhedrosis virus, the TATA-binding protein (TBP) of the insect host cell likely participates in early viral transcription, which is mediated by the host RNA polymerase II. However, the role of TBP in late and very late viral transcription, which is accomplished by an alpha-amanitin-resistant RNA polymerase, is unclear. We observed a dramatic increase of TBP protein during the late phases of infection. TBP mRNA levels, however, were not coordinately increased. Indirect-immunofluorescence studies revealed a nuclear redistribution of TBP during infection. After labeling of viral replication centers with bromodeoxyuridine (BrdU), costaining of TBP and BrdU showed that TBP localized to viral DNA replication centers. These results suggest a putative role of TBP during late viral transcription, which may occur in close proximity to viral DNA replication.


Subject(s)
DNA Replication , DNA, Viral/biosynthesis , Nucleopolyhedroviruses/metabolism , TATA-Box Binding Protein/metabolism , Animals , Cell Line , Cell Nucleus/metabolism , Moths/virology , Nucleopolyhedroviruses/physiology , TATA-Box Binding Protein/genetics , Transcription, Genetic
6.
J Virol ; 76(10): 5198-207, 2002 May.
Article in English | MEDLINE | ID: mdl-11967334

ABSTRACT

The ie2 gene of Autographa californica multicapsid nuclear polyhedrosis virus is 1 of the 10 baculovirus genes that have been identified as factors involved in viral DNA replication. IE2 is detectable in the nucleus as one of the major early-expressed proteins and exhibits a dynamic localization pattern during the infection cycle (D. Murges, I. Quadt, J. Schröer, and D. Knebel-Mörsdorf, Exp. Cell Res. 264:219-232, 2001). Here, we investigated whether IE2 localized to regions of viral DNA replication. After viral DNA was labeled with bromodeoxyuridine (BrdU), confocal imaging indicated that defined IE2 domains colocalized with viral DNA replication centers as soon as viral DNA replication was detectable. In addition, a subpopulation of IE2 structures colocalized with two further virus-encoded replication factors, late expression factor 3 (LEF-3) and the DNA binding protein (DBP). While DBP and LEF-3 structures always colocalized and enlarged simultaneously with viral DNA replication sites, only those IE2 structures that colocalized with replication sites also colocalized with DBP. Replication and transcription of DNA viruses in association with promyelocytic leukemia protein (PML) oncogenic domains have been observed. By confocal imaging we demonstrated that the human PML colocalized with IE2. Triple staining revealed PML/IE2 domains in the vicinity of viral DNA replication centers, while IE2 alone colocalized with early replication sites, demonstrating that PML structures do not form common domains with viral DNA replication centers. Thus, we conclude that IE2 colocalizes alternately with PML and the sites of viral DNA replication. Small ubiquitin-like modifier SUMO-1 has been implicated in the nuclear distribution of PML. Similar to what was found for mammalian cells, small ubiquitin-like modifiers were recruited to PML domains in infected insect cells, which suggests that IE2 and PML colocalize in conserved cellular domains. In summary, our results support a model for IE2 as part of various functional sites in the nucleus that are connected with viral DNA replication.


Subject(s)
Baculoviridae/physiology , Cell Nucleus/metabolism , DNA Replication , DNA, Viral/biosynthesis , Immediate-Early Proteins/metabolism , Animals , Baculoviridae/genetics , Cell Line , Cell Nucleus/virology , DNA-Binding Proteins/metabolism , Genes, Viral , Insecta , Neoplasm Proteins/metabolism , Nuclear Proteins/metabolism , Nucleopolyhedroviruses/metabolism , Transcription Factors/metabolism , Tumor Suppressor Proteins , Viral Proteins/metabolism , Virus Replication
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