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Biosens Bioelectron ; 26(4): 1631-7, 2010 Dec 15.
Article in English | MEDLINE | ID: mdl-20851593

ABSTRACT

An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.


Subject(s)
Antigens, Human Platelet/genetics , Biosensing Techniques/methods , Blood Platelets/immunology , DNA Barcoding, Taxonomic/methods , Biosensing Techniques/instrumentation , DNA Barcoding, Taxonomic/instrumentation , Fluorescence , Genotype , Humans , Oligonucleotide Array Sequence Analysis/instrumentation , Oligonucleotide Array Sequence Analysis/methods , Oligonucleotide Probes , Polymorphism, Single Nucleotide
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