Developing climate-resilient rice varieties (BRRI dhan97 and BRRI dhan99) suitable for salt-stress environments in Bangladesh.

Salinity variations are the main reason for rice yield fluctuations in salt-prone regions throughout the dry season (Boro season). Plant breeders must produce new rice varieties that are more productive, salt tolerant, and stable across a variety of settings to ensure Bangladesh's food sustainability. To assess the yield and stability, we used fifteen rice genotypes containing two tolerant checks BRRI dhan67, Binadhan-10 and the popular Boro rice variety BRRI dhan28 in different salinity "hotspot" in three successive years followed by additive main effects and multiplicative interaction (AMMI) model utilizing a randomized complete block (RCB) design with two replications. Parents selection was done based on estimated breeding values (EBVs). Eight parents with high EBVs (IR83484-3-B-7-1-1-1, IR87870-6-1-1-1-1-B, BR8992-B-18-2-26, HHZ5-DT20-DT2-DT1, HHZ12-SAL2-Y3-Y2, BR8980-B-1-3-5, BRRI dhan67, and Binadhan-10) might be used to develop new segregating breeding materials. Based on farmer preferences and grain acceptability, three genotypes (IR83484-3-B-7-1-1-1, HHZ5-DT20-DT2-DT1, and HHZ12-SAL2-Y3-Y2) were the winning and best ones. The above three genotypes in the proposed variety trial showed significantly higher yields than the respective check varieties, high salinity tolerance ability, and good grain quality parameters. Among them, HHZ5-DT20-DT2-DT1 and IR83484-3-B-7-1-1-1 harbored eight and four QTL/genes that regulate the valuable traits revealed through 20 SNP genotyping. Finally, two genotypes IR83484-3-B-7-1-1-1 and HHZ5-DT20-DT2-DT1 were released as high salinity-tolerant rice varieties BRRI dhan97 and BRRI dhan99, respectively in Bangladesh for commercial cultivation for sustaining food security and sustainability.

Genetic diversity of Lathyrus sp collected from different geographical regions.

BACKGROUND: The demand for grass peas (Lathyrus sativus L.) had increased as high nutritional safe food, but most of the accessions of South Asia and Africa had low grain harvest. Therefore, this study had been undertaken to collect grass pea germplasm for boosting yields and quality improvement. METHODS AND RESULTS: In this study, 400 accessions of grass pea from different geographical regions had characterized by using 56 Simple Sequences Repeat (SSRs) markers. In total 253 alleles were detected, the maximum and minimum polymorphic information content (PIC) indices were 0.70 and 0.34 found in markers G17922 and G18078, correspondingly. The germplasm was split into two main and one sub-group by cluster assay, by SSR assay, and three populations by model-based population structure analysis (Pop1, Pop2 and admixed). Neighbors joining tree assay showed the tested germplasm highly diverse in structure. Three-dimensional principal components analysis (PCA) and two dimensional principles coordinate analysis (PCoA) exhibited two main and one admixed group (P1, P2 and P1P2). In addition, FST population value of pairwise mean and analysis of molecular variance (AMOVA) showed high population structure across all pairs of populations on an average 0.1710 advocating all population structure categories varied significantly. The average predictable heterozygosity distant was 0.4472-0.4542 in same cluster for the individuals. CONCLUSION: Discovery from this study revealed SSR markers based polymorphic bands showed in the diversified grasspea germplasm which might be utilized as genetic resource of a breeding scheme and prospective uses for mapping analyses of recombinant inbred lines (RIL).